Barbora Papoušková

2011

• B. Papouskova, P. Bednar, K. Hron, J. Stavek, J. Balik, R. Myjavcova, P. Bartak, E. Tomankova, and K. Lemr, “Advanced liquid chromatography/mass spectrometry profiling of
  anthocyanins in relation to set of red wine varieties certified in Czech
  Republic,” JOURNAL OF CHROMATOGRAPHY A, vol. 1218, iss. 42, pp. 7581-7591, 2011.
  [Bibtex]

  @article ISI:000296037900020,
  Author = Papouskova, Barbora and Bednar, Petr and Hron, Karel and Stavek, Jan and
     Balik, Josef and Myjavcova, Renata and Bartak, Petr and Tomankova, Eva
     and Lemr, Karel,
  Title = Advanced liquid chromatography/mass spectrometry profiling of
     anthocyanins in relation to set of red wine varieties certified in Czech
     Republic,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2011,
  Volume = 1218,
  Number = 42,
  Pages = 7581-7591,
  Month = OCT 21,
  Abstract = Hyphenation of micro-liquid and ultra-performance liquid chromatography
     with a hybrid (QqTOF) tandem mass spectrometry was studied from the
     viewpoint of their applicability for monitoring of anthocyanin dyes in
     various cultivars and different vintages of red wine. After appropriate
     optimization both techniques proved to be suitable for this task. UHPLC
     system provided lower LOD and LOQ values as well as higher productivity
     and precision of retention parameters and peak areas with respect to
     micro-LC method. On the other hand, micro-LC method offers significant
     savings of mobile phase, less contamination of ion source of mass
     spectrometer and makes the nebulization process in electrospray much
     easier. In combination with principal component analysis (PCA), the
     method allows to elucidate long-term relations in a complete set of
     certified red wine cultivars grown in South Moravia (certified in Czech
     Republic until year 2005). Among the studied varieties Rubinet exhibit a
     specific anthocyanin profile. Especially interesting is a high content
     of 3-coumaroylglucoside-5-glucosides of malvidin and peonidin. Those
     dyes can be used as markers of artificial color enhancement. Principal
     component analysis applied to data processed with regard to enzymatic
     activity markedly facilitate classification among varieties. This
     approach allows resolution of teinturiers. variety Blaufrankish and
     partial classification of other varieties. (C) 2011 Elsevier B.V. All
     rights reserved.,
  DOI = 10.1016/j.chroma.2011.07.027,
  ISSN = 0021-9673,
  Unique-ID = ISI:000296037900020,
  

• P. Kosina, J. Vacek, B. Papouskova, M. Stiborova, J. Styskala, P. Cankar, E. Vrublova, J. Vostalova, V. Simanek, and J. Ulrichova, “Identification of benzo[c]phenanthridine metabolites in human
  hepatocytes by liquid chromatography with electrospray ion-trap and
  quadrupole time-of-flight mass spectrometry,” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL
  AND LIFE SCIENCES, vol. 879, iss. 15-16, pp. 1077-1085, 2011.
  [Bibtex]

  @article ISI:000290645400008,
  Author = Kosina, Pavel and Vacek, Jan and Papouskova, Barbora and Stiborova,
     Marie and Styskala, Jakub and Cankar, Petr and Vrublova, Eva and
     Vostalova, Jitka and Simanek, Vilim and Ulrichova, Jitka,
  Title = Identification of benzo[c]phenanthridine metabolites in human
     hepatocytes by liquid chromatography with electrospray ion-trap and
     quadrupole time-of-flight mass spectrometry,
  Journal = JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL
     AND LIFE SCIENCES,
  Year = 2011,
  Volume = 879,
  Number = 15-16,
  Pages = 1077-1085,
  Month = MAY 1,
  Abstract = The metabolism of the benzo[c]phenanthridine alkaloids was studied
     using human hepatocytes which are an excellent model system for
     biotransformation studies. For analysis of the alkaloids and their
     metabolites, an electrospray quadrupole ion-trap mass spectrometry (ESI
     ion-trap MS) connected to a reversed phase chromatographic system based
     on cyanopropyl modified silica was used. The optimized experimental
     protocol allowed simultaneous analysis of the alkaloids and their
     metabolites and enabled study of their uptake into and interconversion
     in human hepatocytes. The results show that formation of the dihydro
     metabolite which may be followed by specific
     O-demethylenation/O-demethylation processes, is probably the main route
     of biotransformation (detoxification) of the benzo[c]phenanthridines
     in human hepatocytes. The structure of the main O-demethyl metabolite
     (2-methoxy-12-methyl-12,13-dihydro[1,3]dioxolo[4',5':4,5]benzo[1,2
     -c]phenanthridin-1-ol; 336.1 m/z,) was proposed by the multi-stage MS
     and quadrupole time-of-flight MS methods using chemically synthesized
     standard. (C) 2011 Elsevier B.V. All rights reserved.,
  DOI = 10.1016/j.jchromb.2011.03.023,
  ISSN = 1570-0232,
  Unique-ID = ISI:000290645400008,
  

2010

• R. Myjavcova, P. Marhol, V. Kren, V. Simanek, J. Ulrichova, I. Palikova, B. Papouskova, K. Lemr, and P. Bednar, “Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using
  chromatographic fractionation followed by microcolumn liquid
  chromatography-mass spectrometry,” JOURNAL OF CHROMATOGRAPHY A, vol. 1217, iss. 51, pp. 7932-7941, 2010.
  [Bibtex]

  @article ISI:000285429700002,
  Author = Myjavcova, Renata and Marhol, Petr and Kren, Vladimir and Simanek, Vilim
     and Ulrichova, Jitka and Palikova, Irena and Papouskova, Barbora and
     Lemr, Karel and Bednar, Petr,
  Title = Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using
     chromatographic fractionation followed by microcolumn liquid
     chromatography-mass spectrometry,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2010,
  Volume = 1217,
  Number = 51,
  Pages = 7932-7941,
  Month = DEC 17,
  Note = 25th International Symposium on Microscale BioSeparations (MSB 2010),
     Prague, CZECH REPUBLIC, MAR 21-25, 2010,
  Abstract = Anthocyanins from the fruit Lonicera caerulea L var. kamtschatica
     (blueberry honeysuckle, Caprifoliaceae) were studied via
     (semi)preparative chromatographic fractionation followed by MS and mu
     LC/MS analysis. The extraction procedure was optimized with respect to
     analytical purposes as well as its potential use for the preparation of
     nutraceuticals. The highest yield of anthocyanins was obtained using
     acidified methanol as the extraction medium. A comparable total
     anthocyanin content was obtained using a mixture of methanol and
     acetone. However, when Lonicera anthocyanins were in contact with
     acetone, a condensation reaction occurred to a large extent and related
     5-methylpyranoanthocyanins were found. The effect of other extraction
     media, including ethanol as a ``green'' solvent, is also discussed.
     The potential of two fractionation procedures for extract purification
     differing in their chromatographic selectivity and scale was studied
     (i.e. using a Sephadex LH-20 gel column and a reversed phase). Fractions
     obtained by both procedures were used for a detailed analysis. MS and mu
     LC/MS(2) methods were used for monitoring anthocyanin and
     5-methylpyranoderivatives content as well as identifying less common and
     more complex dyes (dimer of cyanidin-3-hexoside,
     cyanidin-ethyl-catechin-hexosides, etc.). These more complex dyes are
     most likely formed during fruit treatment. (C) 2010 Elsevier B.V. All
     rights reserved.,
  DOI = 10.1016/j.chroma.2010.05.058,
  ISSN = 0021-9673,
  Unique-ID = ISI:000285429700002,
  

• L. Hartmanova, V. Ranc, B. Papouskova, P. Bednar, V. Havlicek, and K. Lemr, “Fast profiling of anthocyanins in wine by desorption nano-electrospray
  ionization mass spectrometry,” JOURNAL OF CHROMATOGRAPHY A, vol. 1217, iss. 25, SI, pp. 4223-4228, 2010.
  [Bibtex]

  @article ISI:000278779000031,
  Author = Hartmanova, Lucie and Ranc, Vaclav and Papouskova, Barbora and Bednar,
     Petr and Havlicek, Vladimir and Lemr, Karel,
  Title = Fast profiling of anthocyanins in wine by desorption nano-electrospray
     ionization mass spectrometry,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2010,
  Volume = 1217,
  Number = 25, SI,
  Pages = 4223-4228,
  Month = JUN 18,
  Abstract = Desorption electrospray ionization (DESI) mass spectrometry appears to
     be a useful technique applicable in different areas (e.g. analysis of
     pharmaceuticals, identification of biologically active compounds in
     tissues, imaging mass spectrometry). Its modification termed desorption
     nano-electrospray (nano-DESI) was tested for analysis of anthocyanins.
     Acidifying of samples and acidic spray liquid (methanol:water = 75:25
     with 0.2\% HCOOH) were essential for obtaining good quality spectra.
     Profiles of main anthocyanins in wine samples, two vintages (2005 and
     2007) of three cultivars (Alibernet, Neronet and Rubinet), were
     successfully acquired. They were in agreement with results of LC/MS
     experiments (anthocyanins isolated by solid phase extraction were
     separated by mu-HPLC with gradient elution and detected by ESI-MS).
     Nano-DESI-MS data also allowed to determine ratio of two cultivars
     (Neronet and Rubinet) in their mixture and to detect coloring of wine by
     tenturier or elderberry extract. Detection of main anthocyanins in
     slices of wine grape, chokeberries and elderberries or in a wine stain
     on cotton fabric is also presented. (C) 2010 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.chroma.2010.03.018,
  ISSN = 0021-9673,
  Unique-ID = ISI:000278779000031,
  

• S. Pirildar, N. Sutlupmar, B. Atasever, S. Erdem-Kuruca, B. Papouskova, and V. Simanek, “Chemical constituents of the different parts of Colchicum baytopiorum
  and their cytotoxic activities on K562 and HL60 cell lines,” PHARMACEUTICAL BIOLOGY, vol. 48, iss. 1, pp. 32-39, 2010.
  [Bibtex]

  @article ISI:000273992300005,
  Author = Pirildar, S. and Sutlupmar, N. and Atasever, B. and Erdem-Kuruca, S. and
     Papouskova, B. and Simanek, V.,
  Title = Chemical constituents of the different parts of Colchicum baytopiorum
     and their cytotoxic activities on K562 and HL60 cell lines,
  Journal = PHARMACEUTICAL BIOLOGY,
  Year = 2010,
  Volume = 48,
  Number = 1,
  Pages = 32-39,
  Month = JAN,
  Abstract = The plant chemistry and cytotoxic activity of Colchicum baytopiorum CD
     Brickell (Liliaceae/Colchicaceae), an endemic species growing in Turkey,
     has been studied for the first time. Nine known alkaloids were isolated
     and their structures were identified by spectral methods (UV, IR,
     (1)H-NMR, and ESI/MS), and the presence of three alkaloids, which could
     not be isolated from the plant, was also detected by LC/MS/MS
     spectrometry. Phenolic acids were elaborated using LC/MS and 11 phenolic
     acids were identified. The presence of two flavonoids appeared to be
     valuable for chemotaxonomic purposes. Guided by the brine shrimp
     lethality test (BSLT), methanol extracts were tested for cytotoxic
     activity by colorimetric MTT test on K562 and HL60 cells. Except the
     seed extract, all methanol extracts showed more cytotoxic activity on
     HL60 cells (IC(50) : 6.5-< 0.1 mu g/mL) than on K562 cells (IC(50) : >
     500-44 mu g/mL).,
  DOI = 10.3109/13880200903029373,
  ISSN = 1388-0209,
  Unique-ID = ISI:000273992300005,
  

2009

• B. Papouskova, P. Bednar, J. Styskala, J. Hlavac, P. Bartak, and K. Lemr, “Mass spectrometry as a tool for characterization of
  N,N-dialkylaminoethane-2-thiols – precursors and degradation products of
  chemical warfare agents,” JOURNAL OF MASS SPECTROMETRY, vol. 44, iss. 11, pp. 1604-1612, 2009.
  [Bibtex]

  @article ISI:000272150300008,
  Author = Papouskova, Barbora and Bednar, Petr and Styskala, Jakub and Hlavac, Jan
     and Bartak, Petr and Lemr, Karel,
  Title = Mass spectrometry as a tool for characterization of
     N,N-dialkylaminoethane-2-thiols - precursors and degradation products of
     chemical warfare agents,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2009,
  Volume = 44,
  Number = 11,
  Pages = 1604-1612,
  Month = NOV,
  Abstract = N,N-dialkylaminoethane-2-thiols belong to the group of precursors and
     degradation products of chemical warfare agents (CWAs). These compounds
     were analyzed by means of electrospray ionization-multiple stage mass
     spectrometry (ion trap) and proposed fragments were confirmed by
     accurate mass measurement using a QqTOF system. The fragmentation
     pathways of studied compounds and the products of oxidation (formation
     of -S-S- linkage) were described. Some minor interesting processes, such
     as rearrangement of SH group, were observed and proved. A new mu LC/MS
     method, based on ion-pairing chromatography, was developed.
     Trifluoroacetic acid was employed as an ion-pairing agent to increase
     the low retention of compounds of interest in the reverse-phase system.
     The technique was compared with the UPLC/MS method, allowing fast
     analysis of all the studied thiols as well as an explorative control of
     originated disulfides. Copyright (C) 2009 John Wiley \& Sons, Ltd.,
  DOI = 10.1002/jms.1674,
  ISSN = 1076-5174,
  Unique-ID = ISI:000272150300008,
  

• B. Uhnakova, A. Petrickova, D. Biedermann, L. Homolka, V. Vejvoda, P. Bednar, B. Papouskova, M. Sulc, and L. Martinkova, “Biodegradation of brominated aromatics by cultures and laccase of
  Trametes versicolor,” CHEMOSPHERE, vol. 76, iss. 6, pp. 826-832, 2009.
  [Bibtex]

  @article ISI:000269243900015,
  Author = Uhnakova, B. and Petrickova, A. and Biedermann, D. and Homolka, L. and
     Vejvoda, V. and Bednar, P. and Papouskova, B. and Sulc, M. and
     Martinkova, L.,
  Title = Biodegradation of brominated aromatics by cultures and laccase of
     Trametes versicolor,
  Journal = CHEMOSPHERE,
  Year = 2009,
  Volume = 76,
  Number = 6,
  Pages = 826-832,
  Month = AUG,
  Abstract = 2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3),
     2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobis phenol
     A (6) (1 mM each) added to growing submerged cultures of Trametes
     versicolor CCBAS 612 were eliminated by 65-85\% from the culture medium
     within 4 d. Extracellular laccase activity in the culture medium was
     influenced by the type of brominated compound added. Maximum level of
     laccase (63 U L(-1)) was found in the culture with 2-bromophenol.
     Tetrabromobisphenol A was degraded by a commercial laccase from Trametes
     versicolor in absence of any oxidation mediator, hydroxylated
     dibrominated compounds being detected as soluble reaction products by
     LC/MS. A significant degradation of brominated phenols by laccase was
     achieved only in the presence of ABTS structural characterization of
     major products suggesting reaction between bromophenol and ABTS
     radicals. (C) 2009 Elsevier Ltd. All rights reserved.,
  DOI = 10.1016/j.chemosphere.2009.04.016,
  ISSN = 0045-6535,
  Unique-ID = ISI:000269243900015,
  

2008

• P. Henklova, R. Vrzal, B. Papouskova, P. Bednar, P. Jancova, E. Anzenbacherova, J. Ulrichova, P. Maurel, P. Pavek, and Z. Dvorak, “SB203580, a pharmacological inhibitor of p38 MAP kinase transduction
  pathway activates ERK and JNK MAP kinases in primary cultures of human
  hepatocytes,” EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 593, iss. 1-3, pp. 16-23, 2008.
  [Bibtex]

  @article ISI:000259508100003,
  Author = Henklova, Pavla and Vrzal, Radim and Papouskova, Barbora and Bednar,
     Petr and Jancova, Petra and Anzenbacherova, Eva and Ulrichova, Jitka and
     Maurel, Patrick and Pavek, Petr and Dvorak, Zdenek,
  Title = SB203580, a pharmacological inhibitor of p38 MAP kinase transduction
     pathway activates ERK and JNK MAP kinases in primary cultures of human
     hepatocytes,
  Journal = EUROPEAN JOURNAL OF PHARMACOLOGY,
  Year = 2008,
  Volume = 593,
  Number = 1-3,
  Pages = 16-23,
  Month = SEP 28,
  Abstract = Mitogen-activated protein kinases (MAPKs) were extensively studied in
     cancer-derived cell lines; however, studies in non-transformed human
     cells are scarce. In the current paper, we studied the effect of
     SB203580, a pharmacological inhibitor of p38 MAPK, on activation and
     inhibition of p38 MAPK transduction partway in primary human hepatocytes
     (in vitro model of differentiated cells) in comparison with several
     tumor cell lines (proliferating non-differentiated in vitro model). In
     addition, we analyzed the effect of SB203580 on extracellular-regulated
     protein kinase (ERK) and c-jun-N-terminal kinase (JNK) pathways both in
     primary human hepatocytes and tumor cell lines employing primary
     antibodies detecting phosphorylated kinases. We show that SB203580
     activates ERK and JNK in primary cultures of human hepatocytes. The
     levels of ERK-P(Thr202/Tyr204),JNK-P(Thr183/Tyr185) and
     c-Jun-P(Ser63/73), a target down-stream protein of JNK, were increased
     by SB203580. In contrast, SB203580 activated ERK but not JNK in HepG2,
     HL-60, Saos-2 and HaCaT human cancer cell lines. We tested, whether the
     effects of SB203580 are due to metabolism. Using liquid
     chromatography/mass spectrometry, we found one minor metabolite in human
     liver microsomes but not in HepG2 cells. These data imply that
     biotransformation could be responsible for the effects of SB203580 in
     human hepatocytes. This study is the first report on the effects of MAPK
     activators (sorbitol, anisomycin, EGF) and MAPK inhibitors in primary
     human hepatocytes. We observed differential effects of these compounds
     in primary human hepatocytes and in cancer cells, implying the cell-type
     specificity and the essential differences between the role and function
     of MAPKs in normal and cancer cells. (C) 2008 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.ejphar.2008.07.007,
  ISSN = 0014-2999,
  Unique-ID = ISI:000259508100003,
  

2007

• B. Papouskova, P. Bednar, L. Frysova, J. Styskala, J. Hlavac, P. Bartak, J. Ulrichova, J. Jirkovsky, and K. Lemr, “Mass spectrometric study of selected precursors and degradation products
  of chemical warfare agents,” JOURNAL OF MASS SPECTROMETRY, vol. 42, iss. 12, pp. 1550-1561, 2007.
  [Bibtex]

  @article ISI:000252511900003,
  Author = Papouskova, Barbora and Bednar, Petr and Frysova, Lveta and Styskala,
     Jakub and Hlavac, Jan and Bartak, Petr and Ulrichova, Jitka and
     Jirkovsky, Jaromir and Lemr, Karel,
  Title = Mass spectrometric study of selected precursors and degradation products
     of chemical warfare agents,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2007,
  Volume = 42,
  Number = 12,
  Pages = 1550-1561,
  Month = DEC,
  Note = 25th Informal Meeting on Mass Spectrometry (IMMS), Nyiregyhaza-Sosto,
     HUNGARY, MAY 06-10, 2007,
  Organization = Natl Sci Council, Taiwan; Natl Sun Tat-Sen Univ; Org Prohibition Chem
     Weapons; State Off Nucl Safety; Minist Educ, Youth \& Sports,
  Abstract = Selected precursors and degradation products of chemical warfare agents
     namely N,N-dialkylaminoethane-2-ols, N,N-dialkylaminoethyl-2-chlorides
     and some of related N-quaternary salts were studied by means of
     electrospray ionization-multiple tandem mass spectrometry (ESI-MSn).
     Proposed structures were confirmed with accurate mass measurement.
     General fragmentation patterns of these compounds are discussed in
     detail and suggested processes are confirmed using deuterated standards.
     The typical processes are elimination of alkene, hydrogen chloride, or
     water, respectively. Besides, elimination of ethene from propyl chain
     under specific conditions was observed and unambiguously confirmed using
     exact mass measurement and labelled standard. The potential of mass
     spectrometry to distinguish the positional isomers occurring among the
     studied compounds is reviewed in detail using two different MS
     instruments (i.e. ion trap and hybrid quadrupole-time of flight (Q-TOF)
     analyzer). A new microcolumn liquid chromatography (mu LC)/MSn method
     was designed for the cases where the resolution based solely on
     differences in fragmentation is not sufficient. Low retention of the
     derivatives on reversed phase (RP) was overcome by using addition of
     less typical ion pairing agent (1 mm/l, 3,5-dinitrobenzoic acid) to the
     mobile phase (mixture water: acetonitrile). Copyright (C) 2007 John
     Wiley \& Sons, Ltd.,
  DOI = 10.1002/jms.1319,
  ISSN = 1076-5174,
  Unique-ID = ISI:000252511900003,
  

• P. Jancova, E. Anzenbacherova, B. Papouskova, K. Lemr, P. Luzna, A. Veinlichova, P. Anzenbacher, and V. Simanek, “Silybin is metabolized by cytochrome P4502C8 in vitro,” DRUG METABOLISM AND DISPOSITION, vol. 35, iss. 11, pp. 2035-2039, 2007.
  [Bibtex]

  @article ISI:000250282400008,
  Author = Jancova, Petra and Anzenbacherova, Eva and Papouskova, Barbora and Lemr,
     Karel and Luzna, Pavla and Veinlichova, Alena and Anzenbacher, Pavel and
     Simanek, Vilim,
  Title = Silybin is metabolized by cytochrome P4502C8 in vitro,
  Journal = DRUG METABOLISM AND DISPOSITION,
  Year = 2007,
  Volume = 35,
  Number = 11,
  Pages = 2035-2039,
  Month = NOV,
  Abstract = Silybin (a flavonolignan, the main component of silymarin, an extract
     from the seeds of Silybum marianum) has been used to date mostly as a
     hepatoprotectant. However, it also has other interesting activities, e.
     g., anticancer and hypocholesterolemic effects. It is also known that
     silybin can inhibit the activities of the cytochrome P450 (P450)
     enzymes. In this study, a weak interaction of silybin with human
     microsomal CYP2E1, 2A6, 2B6, 2C19, and 2D6 (IC50 >= 250 mu M) was found;
     a moderate inhibition was observed for CYP1A2 and 2C8. The most
     prominent inhibition effect was found with CYP3A4 and CYP2C9 (IC50 <= 50
     mu M). Using mass spectometry detection, production of O-demethylated
     (the main metabolite) as well as hydroxylated derivatives of silybin
     formed by P450 enzymes was detected. The effect of different P450
     inhibitors on the formation of O-demethylated product was also studied.
     In particular, a relatively specific inhibitor of CYP2C8 (quercetin)
     markedly inhibited the formation of this metabolite. With the help of
     recombinant enzymes (bactosomes), it was confirmed that the CYP2C8
     enzyme is responsible for the reaction leading to O-demethylated
     silybin.,
  DOI = 10.1124/dmd.107.016410,
  ISSN = 0090-9556,
  Unique-ID = ISI:000250282400008,
  

2006

• B. Papouskova, P. Bednar, P. Bartak, P. Frycak, J. Sevcik, Z. Stransky, and K. Lemr, "Utilisation of separation methods in the analysis of chemical warfare
  agents," JOURNAL OF SEPARATION SCIENCE, vol. 29, iss. 11, pp. 1531-1538, 2006.
  [Bibtex]

  @article ISI:000239574100001,
  Author = Papouskova, Barbora and Bednar, Petr and Bartak, Petr and Frycak, Petr
     and Sevcik, Juraj and Stransky, Zdenek and Lemr, Karel,
  Title = Utilisation of separation methods in the analysis of chemical warfare
     agents,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2006,
  Volume = 29,
  Number = 11,
  Pages = 1531-1538,
  Month = JUL,
  Abstract = Chemical warfare agents and their degradation products represent a broad
     group of compounds with different chemical properties (polarity,
     volatility, thermostability, etc.). These chemicals often have to be
     detected and determined in complex matrices and therefore highly
     efficient separation techniques hyphenated to selective and sensitive
     detectors play an indispensable role. This review offers an overview of
     selected papers devoted to the title subject. It cannot be considered as
     a comprehensive literature compilation but should allow the reader to
     obtain an insight into the application of separation techniques in the
     important area of human protection and control of chemical weapons.,
  DOI = 10.1002/jssc.200500432,
  ISSN = 1615-9306,
  Unique-ID = ISI:000239574100001,
  

2005

• P. Bednar, B. Papouskova, L. Muller, P. Bartak, J. Stavek, P. Pavlousek, and K. Lemr, "Utilization of capillary electrophoresis/mass spectrometry (CE/MSn) for
  the study of anthocyanin dyes," JOURNAL OF SEPARATION SCIENCE, vol. 28, iss. 12, pp. 1291-1299, 2005.
  [Bibtex]

  @article ISI:000231513900006,
  Author = Bednar, P and Papouskova, B and Muller, L and Bartak, P and Stavek, J
     and Pavlousek, P and Lemr, K,
  Title = Utilization of capillary electrophoresis/mass spectrometry (CE/MSn) for
     the study of anthocyanin dyes,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2005,
  Volume = 28,
  Number = 12,
  Pages = 1291-1299,
  Month = AUG,
  Note = Symposium on Advances in Chromatography and Electrophoresis and
     Chiranal, Palacky Univ, Fac Phys Culture, Olomouc, CZECH REPUBLIC, FEB
     07-10, 2005,
  Organization = Palacky Univ, Fac Sci Dept Analyt Chem; Czech Chem Soc, Chromatog \&
     Electrophoresis Grp,
  Abstract = Hyphenation of capillary electrophoresis with electrospray ionization
     mass spectrometry was utilized for the monitoring of anthocyanins in
     wine and wine musts. CE/MS was performed in two electrolytes: 1) an
     acidic one (chloroacetate-ammonium, pH 2) and 2) a basic one with high
     selectivity towards derivatives containing vicinal hydroxy groups
     (borate-ammonium, pH 9). The setup of MS was optimized and the
     fragmentation of common anthocyanins was studied in detail. Attention
     was also focused on the fragmentation of anthocyanidin skeleton. The
     anthocyanidins substituted with hydroxy groups fragment via a cascade of
     neutral losses of water and carbon monoxide. Fragmentation of
     anthocyanidins containing a methoxy group on their B-ring starts with
     the cleavage of methane and/or methyl radical. The optimized method was
     utilized for the monitoring of changes in anthocyanin profile in red
     wines as well as the process of release of anthocyanins to wine must.,
  DOI = 10.1002/jssc.200500071,
  ISSN = 1615-9306,
  Unique-ID = ISI:000231513900006,