Barbora Papoušková


Address: 17. listopadu 12, Olomouc, 771 46, Czech Republic
Phone: +420 58 563 4447

 Degrees and Professional Qualifications:

  • since 2013 –  deputy head of R-lab, RCPTM
  • since 2012 – Junior Researcher, Regional Centre of Advanced Technologies and Materials (RCPTM)
  • since 2007- Postdoc position, Department of Analytical Chemistry, UPOL
  • 2009 – Ph.D. in analytical chemistry, Palacký University Olomouc
  • 2008 – RNDr. in analytical chemistry, Palacký University Olomouc
  • 2005 – Mgr. in analytical chemistry, Palacký University Olomouc

International Experience:

  • 2013, 2012, 2011 –  Department of Chemistry and Biochemistry, University of Texas, Arlington, USA; Assoc. Prof. Kevin A. Schug (5 months in total)
  • 2007 – Department of Analytical Chemistry and Food Chemistry, University of Vienna, Rakousko; Prof. Wolfgang Lindner (3 months)

Research activities:

  • Hyphenation of liquid separation techniques  (liquid chromatography, ultra-performance liquid chromatography, SFC) with mass spectrometry (HRMS, IMS, QqQ)
  • Principles of separation in liquid phase and their applications for trace analysis in biological matrix
  • Metabolomic studies using (U)HPLC/MS
  • Analysis of plant material focused on  secondary plant materials
  • Tandem mass spectrometry, MALDI imaging, ESI/APCI/APPI-MS for characterization of newly synthesized compounds


  • 27 papers in impacted journals
  • 1 chapter in textbook
  • 30+ conference and seminar contributions
  • 210 citations (without self-citations)
  • 9 h-index

10 selected publications:

  1. Vrba J., Papouskova B., Pyszkova M., Zatloukalova M., Lemr K., Ulrichova J., Vacek J.: Metabolism of palmatine by human hepatocytes and recombinant cytochromes P450. J. Pharm. Biomed. Anal. 102, 193-198 (2015).
  2. Papouskova B., Fan H., Lemr K., Schug K.A.: Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method. J. Sep. Sci. 37, 2192-2199 (2014).
  3. Fan H., Papouskova B., Lemr K., Wigginton J.G., Schug K.A.: Bulk derivatization and direct injection of human cerebrospinal fluid for trace-level quantification of endogenous estrogens using trap-and-elute liquid chromatography with tandem mass spectrometry. J.Sep. Sci. 37, 2010-2017 (2014).
  4. Vacek J., Papouskova B., Kosina P., Galandakova A ., Ulrichova J.: Mass spectrometric investigation of chelerythrine and dihydrochelerythrine biotransformation patterns in human hepatocytes. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 941, 17-24 (2013).
  5. Vacek J., Papouskova B., Vrba J., Zatloukalova M., Kren V., Ulrichova J.: LC-MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model. J. Pharm. Biomed. Anal. 86, 135-142 (2013).
  6. Vrba, J, Gažák R., Kuzma M., Papoušková B., Vacek J.,  Weiszenstein M., Křen V., Ulrichová J.:A Novel Semisynthetic Flavonoid 7-O-Galloyltaxifolin Upregulates Heme Oxygenase-1 in RAW264.7 Cells via MAPK/Nrf2 Pathway. J. Med. Chem. 56, 856−866 (2013).
  7. Papouskova B., Bednar P., Hron K., Stavek, J., Balik, J., Myjavcova, R., Bartak, P., Tomankova, E., Lemr, K.: Advanced liquid chromatography/mass spectrometry profiling of anthocyanins in relation to set of red wine varieties certified in Czech Republic. J. Chromatogr A 1218, 7581-7591 (2011).
  8. Kosina, P., Vacek, J., Papouskova, B., Stiborova, M., Styskala, J., Cankar, P., Vrublova, E., Vostalova, J., Simanek, V., Ulrichova, J.: Identification of benzo[c]phenanthridine metabolites in human hepatocytes by liquid chromatography with electrospray ion-trap and quadrupole time-of-flight mass spectrometry. J. Chromatogr B 879, 1077-1085 (2011).
  9. Papoušková B., Bednář P., Fryšová I., Stýskala J., Hlaváč J., Barták P., Ulrichová J., Jirkovský J., Lemr K.: Mass spectrometric study of selected precursors and degradation products of chemical warfare agents. J. Mass Spectrom. 42, 1550–1561 (2007).
  10. Bednář P., Papoušková B., Műller L., Barták P., Stávek J., Pavloušek P., Lemr K.: Utilization of capillary electrophoresis/mass spectrometry (CE/MSn) for the study of anthocyanin dyes. J. Sep. Sci. 28, 1291-1299 (2005).