Jan Petr

2011

• J. Znaleziona, V. Maier, J. Petr, J. Chrastina, and J. Sevcik, “MEKC Determination of Nilutamide in Human Serum Using Sweeping in High
  Salt Sample Matrix,” CHROMATOGRAPHIA, vol. 74, iss. 1-2, pp. 151-155, 2011.
  [Bibtex]

  @article ISI:000291745400019,
  Author = Znaleziona, Joanna and Maier, Vitezslav and Petr, Jan and Chrastina, Jan
     and Sevcik, Juraj,
  Title = MEKC Determination of Nilutamide in Human Serum Using Sweeping in High
     Salt Sample Matrix,
  Journal = CHROMATOGRAPHIA,
  Year = 2011,
  Volume = 74,
  Number = 1-2,
  Pages = 151-155,
  Month = JUL,
  Abstract = The determination of the antiandrogen drug nilutamide in human serum by
     MEKC using flutamide as an internal standard is described. Several
     parameters influencing the separation, such as the running electrolyte
     composition concerning the micelle concentration and pH, are discussed.
     MEKC separation was achieved within 7 min using 50 mM sodium borate pH
     9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The
     proposed method was applied to determination of nilutamide in spiked
     human serum samples after protein precipitation with acetonitrile. The
     increasing of sensitivity for determination of nilutamide in serum was
     done by sweeping in a high salt concentration sample matrix when the
     injection of a larger volume of sample diluted in 150 mM NaCl was
     applied. The limit of detection after the preconcentration step for
     nilutamide was 26 mu g L(-1).,
  DOI = 10.1007/s10337-011-2019-1,
  ISSN = 0009-5893,
  Unique-ID = ISI:000291745400019,
  

2010

• K. Vitkova, J. Petr, V. Maier, J. Znaleziona, and J. Sevcik, “Study of electromigration effects on a pH boundary during the on-line
  electrokinetic preconcentration by capillary electrophoresis,” ELECTROPHORESIS, vol. 31, iss. 16, pp. 2771-2777, 2010.
  [Bibtex]

  @article ISI:000281732900010,
  Author = Vitkova, Katerina and Petr, Jan and Maier, Vitezslav and Znaleziona,
     Joanna and Sevcik, Juraj,
  Title = Study of electromigration effects on a pH boundary during the on-line
     electrokinetic preconcentration by capillary electrophoresis,
  Journal = ELECTROPHORESIS,
  Year = 2010,
  Volume = 31,
  Number = 16,
  Pages = 2771-2777,
  Month = AUG,
  Abstract = A contribution to the description of electrokinetic effects on the pH
     boundary formed by sodium borate pH 9.5 and sodium phosphate pH 2.5
     electrolytes for on-line preconcentration of weak acids is presented in
     this article. Simulations of electrokinetic injections together with
     experimental studies using contactless conductivity detection verified
     that the preconcentration is induced mainly by dissociation changes of
     analytes on the pH boundary and transient ITP state. Moreover, a study
     of the addition of organic solvent to the injection electrolyte was
     performed with impressive results. Subnanomolar LODs of hydroxybenzoic
     acids were achieved with 80\% of methanol in the injection electrolyte
     which represents more than 70 000-fold preconcentration in comparison
     with classical CZE method.,
  DOI = 10.1002/elps.200900663,
  ISSN = 0173-0835,
  Unique-ID = ISI:000281732900010,
  

• K. Vitkova, M. Pechova, J. Petr, J. Znaleziona, V. Ranc, V. Maier, and J. Sevcik, “A fast determination of yohimbine in pharmaceuticals by micellar
  electrokinetic chromatography,” CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, vol. 8, iss. 2, pp. 273-277, 2010.
  [Bibtex]

  @article ISI:000276548300005,
  Author = Vitkova, Katerina and Pechova, Marketa and Petr, Jan and Znaleziona,
     Joanna and Ranc, Vaclav and Maier, Vitezslav and Sevcik, Juraj,
  Title = A fast determination of yohimbine in pharmaceuticals by micellar
     electrokinetic chromatography,
  Journal = CENTRAL EUROPEAN JOURNAL OF CHEMISTRY,
  Year = 2010,
  Volume = 8,
  Number = 2,
  Pages = 273-277,
  Month = APR,
  Abstract = A method for the fast determination of yohimbine, a potent
     adrenoreceptor antagonist used for the treatment of sexual dysfunctions,
     is proposed in this article. MEKC under basic and acidic conditions
     (sodium borate, pH 9.5 and sodium phosphate, pH 2.5) with SDS was
     developed. The effect of the experimental parameters, e. g. pH, SDS
     concentration and injection time, on yohimbine migration was also
     studied. Both methods were validated in terms of linearity, limits of
     detection and quantification, accuracy, and precision using caffeine as
     an internal standard. The application for the determination of yohimbine
     in hand-made medicaments is also investigated in this study.,
  DOI = 10.2478/s11532-009-0149-1,
  ISSN = 1895-1066,
  Unique-ID = ISI:000276548300005,
  

2009

• J. Petr, O. Ryparova, J. Znaleziona, V. Maier, and J. Sevcik, “On-line combination of CE and microscopy: An insight into the migration
  of microorganisms,” ELECTROPHORESIS, vol. 30, iss. 22, pp. 3863-3869, 2009.
  [Bibtex]

  @article ISI:000272474900009,
  Author = Petr, Jan and Ryparova, Olga and Znaleziona, Joanna and Maier, Vitezslav
     and Sevcik, Juraj,
  Title = On-line combination of CE and microscopy: An insight into the migration
     of microorganisms,
  Journal = ELECTROPHORESIS,
  Year = 2009,
  Volume = 30,
  Number = 22,
  Pages = 3863-3869,
  Month = NOV,
  Abstract = identification of microbial contamination by CE has interested many
     researchers mainly because of the high speed of CE analysis. However,
     the CE separation of such big structures brings a lot of questions
     mainly about the behavior of microorganisms and about the mechanism of
     separation. In this work, we constructed a simple apparatus where a
     microscope was used as one detector and a UV detector was used as the
     second one and we made the comparison of three typical setups for CE of
     microorganisms. Saccharomyces cerevisiae was chosen as a model
     microorganism and was analyzed in bare fused-silica capillaries,
     covalently modified capillaries and dynamically modified capillaries by
     poly(ethylene oxide) or CTAB. Results showed that the use of CE
     instrument directly connected with a microscope could be advantageous
     for the study of separation mechanisms and/or migration behavior.,
  DOI = 10.1002/elps.200900230,
  ISSN = 0173-0835,
  Unique-ID = ISI:000272474900009,
  

• J. Petr, C. Jiang, J. Sevcik, E. Tesarova, and D. W. Armstrong, “Sterility testing by CE: A comparison of online preconcentration
  approaches in capillaries with greater internal diameters,” ELECTROPHORESIS, vol. 30, iss. 22, pp. 3870-3876, 2009.
  [Bibtex]

  @article ISI:000272474900010,
  Author = Petr, Jan and Jiang, Chunxia and Sevcik, Juraj and Tesarova, Eva and
     Armstrong, Daniel W.,
  Title = Sterility testing by CE: A comparison of online preconcentration
     approaches in capillaries with greater internal diameters,
  Journal = ELECTROPHORESIS,
  Year = 2009,
  Volume = 30,
  Number = 22,
  Pages = 3870-3876,
  Month = NOV,
  Abstract = Detection of microbial contamination is of critical importance in the
     medical and the food industry. Rapid tests for the absence or presence
     of viable microorganisms are in urgent demand. CE is a modem analytical
     technique that can be adapted for rapid screening of microbial
     contamination. However, the small dimensions of capillaries allow the
     introduction of only a small fraction of the sample, which can be
     problematic when examining large samples. In this article, we examine
     the possibilities of introducing larger sample volumes using capillaries
     with greater id together with different stacking techniques. The use of
     0.32 mm id capillary and the injection of 60\% of the capillary volume
     led to approximately 120-fold improvement of the injected sample volume
     over the classical injection 5\% of a 0.10-mm id capillary. The setup we
     described opens new possibilities in sterility testing using CE.,
  DOI = 10.1002/elps.200900299,
  ISSN = 0173-0835,
  Unique-ID = ISI:000272474900010,
  

• V. Maier, J. Znaleziona, D. Jirovsky, J. Skopalova, J. Petr, and J. Sevcik, “Determination of antihyperglycemic drugs in nanomolar concentration
  levels by micellar electrokinetic chromatography with non-ionic
  surfactant,” JOURNAL OF CHROMATOGRAPHY A, vol. 1216, iss. 20, pp. 4492-4498, 2009.
  [Bibtex]

  @article ISI:000265956100025,
  Author = Maier, Vitezslav and Znaleziona, Joanna and Jirovsky, David and
     Skopalova, Jana and Petr, Jan and Sevcik, Juraj,
  Title = Determination of antihyperglycemic drugs in nanomolar concentration
     levels by micellar electrokinetic chromatography with non-ionic
     surfactant,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2009,
  Volume = 1216,
  Number = 20,
  Pages = 4492-4498,
  Month = MAY 15,
  Abstract = A method for the separation of six selected antihyperglycemic
     (antidiabetic) drugs (tolbutamide, gliclazide, glimepiride,
     glibenclamide, repaglinide, and glipizide) was developed with use of
     micellar electrokinetic chromatography. Two non-ionic poly(ethylene
     glycol)-based surfactants Genapol X-080 and Triton X-114 (reduced) were
     studied as neutral pseudostationary phases. High alkaline pH 10.0 was
     used to obtain negative charges of separated antidiabetic drugs and
     non-ionic surfactants were employed for selectivity alteration. Both
     non-ionic surfactants provided good selectivity at concentration 0.2\%
     (v/v) in sodium borate buffer and the separation of six drugs was
     obtained within 5 min. An on-line preconcentration method based on
     reversed electrode polarity switching was employed for the determination
     of anti hyperglycemic drugs in blood serum after acetonitrile protein
     precipitation. The limits of detection ranged from 20.8 nmol L-(1) for
     tolbutamide to 6.5 nmol L(-1) for glibenclamide, respectively. (C) 2009
     Elsevier B.V. All rights reserved.,
  DOI = 10.1016/j.chroma.2009.03.052,
  ISSN = 0021-9673,
  Unique-ID = ISI:000265956100025,
  

• J. Petr, O. Ryparova, V. Ranc, P. Hinnerova, J. Znaleziona, M. Kowalska, R. Knob, V. Maier, I. Frebort, K. Lemr, and J. Sevcik, “Assessment of CE for the identification of microorganisms,” ELECTROPHORESIS, vol. 30, iss. 3, pp. 444-449, 2009.
  [Bibtex]

  @article ISI:000264357500002,
  Author = Petr, Jan and Ryparova, Olga and Ranc, Vaclav and Hinnerova, Petra and
     Znaleziona, Joanna and Kowalska, Marta and Knob, Radim and Maier,
     Vitezslav and Frebort, Ivo and Lemr, Karel and Sevcik, Juraj,
  Title = Assessment of CE for the identification of microorganisms,
  Journal = ELECTROPHORESIS,
  Year = 2009,
  Volume = 30,
  Number = 3,
  Pages = 444-449,
  Month = FEB,
  Abstract = The assessment of capillary electrophoresis for separation and
     identification of microorganisms is described in this article. The work
     brings a comparison of the application of uncoated capillaries modified
     with poly(ethylene oxide) and coated capillaries for the separation of
     model microorganisms Saccharomyces cerevisiae and Escherichia coli with
     Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best
     separation was achieved in the coated capillary using 1 mM
     Tris-citrate-fructose buffer, pH 6.9. A simple identification based on
     the migration time and UV spectrum was found unsatisfactory and thus a
     method based on a post-separation cultivation and sequential analysis
     was developed. Off-line combination with mass spectrometric analysis
     with the use of desorption electrospray was shown to be an interesting
     alternative in a study of microorganisms. Mass spectra allowed
     distinguishing among analyzed cells.,
  DOI = 10.1002/elps.200800232,
  ISSN = 0173-0835,
  Unique-ID = ISI:000264357500002,
  

• J. Petr, V. Maier, J. Znaleziona, K. Vitkova, V. Ranc, and J. Sevcik, “On-line Preconcentration on pH Boundary – the Beauty of Capillary
  Electrophoresis,” CHEMICKE LISTY, vol. 103, iss. 8, pp. 661-666, 2009.
  [Bibtex]

  @article ISI:000269243500006,
  Author = Petr, Jan and Maier, Vitezslav and Znaleziona, Joanna and Vitkova,
     Katerina and Ranc, Vaclav and Sevcik, Juraj,
  Title = On-line Preconcentration on pH Boundary - the Beauty of Capillary
     Electrophoresis,
  Journal = CHEMICKE LISTY,
  Year = 2009,
  Volume = 103,
  Number = 8,
  Pages = 661-666,
  Abstract = The beauty of capillary electrophoresis is shown on preconcentration of
     some phenolics. The method is based on the electrokinetic injection of
     phenolics into a sodium borate electrolyte (pH 9.5) at the pH boundary
     formed by sodium phosphate (pH 2.5) followed by mobilization and
     separation by replacing the injection vial with the vial containing
     micellar environment. This technique allows determination of nanomolar
     concentrations of phenolics.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000269243500006,
  

2008

• J. Petr, K. Vitkova, V. Ranc, J. Znaleziona, V. Maier, R. Knob, and J. Sevcik, “Determination of some phenolic acids in Majorana hortensis by capillary
  electrophoresis with online electrokinetic preconcentration,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 56, iss. 11, pp. 3940-3944, 2008.
  [Bibtex]

  @article ISI:000256445300008,
  Author = Petr, Jan and Vitkova, Katerina and Ranc, Vaclav and Znaleziona, Joanna
     and Maier, Vitezslav and Knob, Radim and Sevcik, Juraj,
  Title = Determination of some phenolic acids in Majorana hortensis by capillary
     electrophoresis with online electrokinetic preconcentration,
  Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
  Year = 2008,
  Volume = 56,
  Number = 11,
  Pages = 3940-3944,
  Month = JUN 11,
  Abstract = An online accumulation/mobilization preconcentration technique based on
     a dynamic pH junction technique and electrokinetic injection was
     employed for analysis of phenolic acids (sinapic; ferulic, coumarinic,
     caffeic, syringic, vanillic, and 4-hydroxybenzoic acid) in extracts from
     Majorana hortensis leaves. Samples were extracted by pressurized solvent
     extraction with acetone at 150 degrees C and 15 MPa. The capillary
     electrophoretic method employed 50 mmol.L-1 sodium borate, pH 9.5, as
     the sample electrolyte, 50 mmol.L-1 sodium phosphate, pH 2.5, as the
     background electrolyte, and 50 mmol.L-1 sodium phosphate, pH 2.5, with
     60 mmol.L-1 sodium dodecyl sulfate as the mobilization electrolyte. The
     method allowed 720-fold to 5560-fold preconcentration of the phenolic
     acids during 30 min of electrokinetic accumulation with detection limits
     from 0.38 to 4.22 ng.mL(-1).,
  DOI = 10.1021/jf8000179,
  ISSN = 0021-8561,
  Unique-ID = ISI:000256445300008,
  

• J. Znaleziona, J. Petr, R. Knob, V. Maier, and J. Sevcik, “Dynamic coating agents in CE,” CHROMATOGRAPHIA, vol. 67, iss. S, pp. S5-S12, 2008.
  [Bibtex]

  @article ISI:000256533700003,
  Author = Znaleziona, Joanna and Petr, Jan and Knob, Radim and Maier, Vitezslav
     and Sevcik, Juraj,
  Title = Dynamic coating agents in CE,
  Journal = CHROMATOGRAPHIA,
  Year = 2008,
  Volume = 67,
  Number = S,
  Pages = S5-S12,
  Note = 10th Symposium on Advances in Chromatography and Electrophoresis and
     Chiranal, Palacky Univ, Olomouc, CZECH REPUBLIC, JUN 24-27, 2007,
  Organization = Czech Chem Soc, Working Grp Chromatog \& Electrophoresis,
  Abstract = In capillary electrophoresis (CE) dynamic coating agents are commonly
     used for manipulating or reversing electroosmotic flow (EOF) and
     suppressing adsorption of analytes by the silica surface. In this paper
     we review general aspects of dynamic coating in CE and discuss features
     and facts concerning formation of the EOF and determination of its
     magnitude.,
  DOI = 10.1365/s10337-007-0509-y,
  ISSN = 0009-5893,
  Unique-ID = ISI:000256533700003,
  

• O. Ryparova, J. Petr, M. Kowalska, J. Znaleziona, R. Knob, V. Maier, I. Frebort, and J. Sevcik, “Analysis of Microorganisms by Capillary Electrophoresis,” CHEMICKE LISTY, vol. 102, iss. 12, pp. 1121-1126, 2008.
  [Bibtex]

  @article ISI:000262061500007,
  Author = Ryparova, Olga and Petr, Jan and Kowalska, Marta and Znaleziona, Joanna
     and Knob, Radim and Maier, Vitezslav and Frebort, Ivo and Sevcik, Juraj,
  Title = Analysis of Microorganisms by Capillary Electrophoresis,
  Journal = CHEMICKE LISTY,
  Year = 2008,
  Volume = 102,
  Number = 12,
  Pages = 1121-1126,
  Abstract = Utilization of capillary electrophoresis for screening identification of
     Escherichia coli in water (faecal contamination) and pharmaceuticals is
     described. The method uses an electrolyte 4.5 mM in Tris, 4.5 mM in
     H(3)BO(3) and 0.1 mM in EDTA with 0.0125 \% poly(ethylene oxide). A
     brief survey of the published methods for analysis of microorganisms by
     capillary electrophoresis is given.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000262061500007,
  

2007

• J. Horakova, J. Petr, V. Maier, J. Znaleziona, A. Stanova, J. Marak, D. Kaniansky, and J. Sevcik, “Combination of large volume sample stacking and dynamic pH junction for
  on-line preconcentration of weak electrolytes by capillary
  electrophoresis in comparison with isotachophoretic techniques,” JOURNAL OF CHROMATOGRAPHY A, vol. 1155, iss. 2, pp. 193-198, 2007.
  [Bibtex]

  @article ISI:000247855200010,
  Author = Horakova, Jana and Petr, Jan and Maier, Vitezslav and Znaleziona, Joanna
     and Stanova, Andrea and Marak, Jozef and Kaniansky, Dusan and Sevcik,
     Juraj,
  Title = Combination of large volume sample stacking and dynamic pH junction for
     on-line preconcentration of weak electrolytes by capillary
     electrophoresis in comparison with isotachophoretic techniques,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2007,
  Volume = 1155,
  Number = 2,
  Pages = 193-198,
  Month = JUL 6,
  Note = 15th International Symposium on Capillary Electroseparation Techniques,
     Paris, FRANCE, AUG 28-30, 2006,
  Abstract = An on-line preconcentration capillary electrophoresis (CE) technique,
     which combines a large volume sample stacking with a dynamic pH junction
     technique, is introduced in this paper. This dynamic pH junction with
     co-electroosmotic migration is formed between sodium borate pH 9.5 and
     sodium phosphate pH 2.5 with 150 mM sodium dodecylsulfate (SDS). A full
     capillary based injection allows determination of weak acidic compounds
     at ppb concentration levels (achieved LOD for benzoic acid was 11 nmol
     L-1). The proposed preconcentration method was compared with ITP/ITP
     (LOD 120 nmol L-1), ITP/CZE (LOD 740 nmol L-1) and a simple CZE method
     (LOD 23,330 nmol L-1). The analytical potential of this method was
     assessed with juice test samples. (C) 2007 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.chroma.2007.01.075,
  ISSN = 0021-9673,
  Unique-ID = ISI:000247855200010,
  

• V. Maier, J. Petr, R. Knob, J. Horakova, and J. Sevcik, “Electrokinetic partial filling technique as a powerful tool for
  enantiomeric separation of DL-lactic acid by CE with contactless
  conductivity detection,” ELECTROPHORESIS, vol. 28, iss. 11, pp. 1815-1822, 2007.
  [Bibtex]

  @article ISI:000247279800020,
  Author = Maier, Vitezslav and Petr, Jan and Knob, Radim and Horakova, Jana and
     Sevcik, Juraj,
  Title = Electrokinetic partial filling technique as a powerful tool for
     enantiomeric separation of DL-lactic acid by CE with contactless
     conductivity detection,
  Journal = ELECTROPHORESIS,
  Year = 2007,
  Volume = 28,
  Number = 11,
  Pages = 1815-1822,
  Month = JUN,
  Abstract = A modified partial filling method for chiral separation Of DL-lactic
     acid as the model chiral compound with vancomycin chloride as the chiral
     selector was developed by CE with contactless conductivity detection.
     Electrokinetic partial filling technique (EK-PFT) was used as an
     altemative method to the conventional hydrodynamic partial filling
     method. EK-PFT, in contrast to the hydrodynamic partial filling
     technique, allowed the removal of the chloride counterions from the
     chiral selector which otherwise led to poor sensitivity in conductivity
     detection. The baseline separation Of DL-lactic acid as the model
     analyte was achieved in 5 min in a polyacrylamide-coated capillary. The
     best resolution was achieved by electrokinetic partial filling of
     vancomycin cations from the injection solution containing 5 nunol/L
     oxalate L-histidiniumat pH 4.5 with 10 mmol/L vancomycin chloride.
     Computer simulation was used to explain the observed phenomena in the
     boundary between the inject vial and the capillary during the EK-PFT of
     vancomycin cations.,
  DOI = 10.1002/elps.200600578,
  ISSN = 0173-0835,
  Unique-ID = ISI:000247279800020,
  

• J. Horakova, J. Petr, V. Maier, E. Tesarova, L. Veis, D. W. Armstrong, B. Gas, and J. Sevcik, “On-line preconcentration of weak electrolytes by electrokinetic
  accumulation in CE: Experiment and simulation,” ELECTROPHORESIS, vol. 28, iss. 10, pp. 1540-1547, 2007.
  [Bibtex]

  @article ISI:000246923300011,
  Author = Horakova, Jana and Petr, Jan and Maier, Vitezslav and Tesarova, Eva and
     Veis, Libor and Armstrong, Daniel W. and Gas, Bohuslav and Sevcik, Juraj,
  Title = On-line preconcentration of weak electrolytes by electrokinetic
     accumulation in CE: Experiment and simulation,
  Journal = ELECTROPHORESIS,
  Year = 2007,
  Volume = 28,
  Number = 10,
  Pages = 1540-1547,
  Month = MAY,
  Abstract = A new on-line preconcentration technique was developed that makes
     possible to determine nanomolar concentrations of weak acidic analytes
     in CE. The method consists of long-running electrokinetic sample
     injection and stacking (electrokinetic immobilization) of the analytes
     at a boundary of two electrolytes with different pH values (pH 9.5 and
     2.5) and consequent mobilization of the stacked uncharged analytes in a
     micellar system (containing SDS micelles). Several factors including
     buffer concentration, pH, applied voltage, time of preconcentration, and
     SDS concentration were tested to optimize the analysis method. An about
     4600-fold increase of the sample concentration (in comparison with the
     standard CZE) can be achieved during the preconcentration step. Two
     preservatives applied in food industry - benzoic acid and sorbic acid
     were used as model samples. The applicability of the proposed method in
     food analysis was demonstrated by determination of nanomolar
     concentrations of benzoic acid in sunflower oil. An extended version of
     the computer program Simul was used for modeling both the
     preconcentration and mobilization processes taking place in the
     capillary.,
  DOI = 10.1002/elps.200600681,
  ISSN = 0173-0835,
  Unique-ID = ISI:000246923300011,
  

2006

• J. Petr, V. Maier, J. Horakova, J. Sevcik, and Z. Stransky, “Capillary isotachophoresis from the student point of view – images and
  the reality,” JOURNAL OF SEPARATION SCIENCE, vol. 29, iss. 18, pp. 2705-2715, 2006.
  [Bibtex]

  @article ISI:000243338800002,
  Author = Petr, Jan and Maier, Vitezslav and Horakova, Jana and Sevcik, Juraj and
     Stransky, Zdenek,
  Title = Capillary isotachophoresis from the student point of view - images and
     the reality,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2006,
  Volume = 29,
  Number = 18,
  Pages = 2705-2715,
  Month = DEC,
  Abstract = A review of some fundamental aspects of ITP from the student point of
     view, imaginations of some basic facts and laws, use of ITP, and the
     recent trends are presented. The results of theoretical computations of
     ITP separation processes are added for comparison of imaginations with
     the exact mathematical description.,
  DOI = 10.1002/jssc.200600249,
  ISSN = 1615-9306,
  Unique-ID = ISI:000243338800002,
  

• J. Petr, V. Maier, J. Horakova, and J. Sevcik, “Simultaneous contactless conductivity detection and UV detection for the
  study of separation of tamsulosin enantiomers in discontinuous
  electrolyte systems by CE,” ELECTROPHORESIS, vol. 27, iss. 23, pp. 4735-4745, 2006.
  [Bibtex]

  @article ISI:000242903000017,
  Author = Petr, Jan and Maier, Vitezslav and Horakova, Jana and Sevcik, Juraj,
  Title = Simultaneous contactless conductivity detection and UV detection for the
     study of separation of tamsulosin enantiomers in discontinuous
     electrolyte systems by CE,
  Journal = ELECTROPHORESIS,
  Year = 2006,
  Volume = 27,
  Number = 23,
  Pages = 4735-4745,
  Month = DEC,
  Abstract = This work shows the potential of using discontinuous electrolyte systems
     for the separation of tamsulosin enantiomers by CE. Sulfated
     P-cyclodextrin was used as a chiral selector. In acidic electrolytes,
     sulfated P-cyclodextrin migrates as an anion and the analyte
     (tamsulosin) migrates as a cation. Due to this, four experimental
     arrangements were proposed. These arrangements differ in composition of
     electrolytes in the inlet compartment, in the capillary and in the
     outlet compartment. The separation of tamsulosin enantiomers in acetate
     buffers with sodium and Tris counterions was studied. Simultaneous
     contactless conductivity detection and UV detection were used for the
     study of the separation mechanism in these systems. Mobilities of
     sulfated beta-cycloclextrin were used for the calculation of the time
     when the analyte migrates through the BGE zone with the selector. The
     simulation program Simul 4.0 was used for the calculations of the
     concentration profiles of the electrolyte components dependent on the
     time of the separation. The mechanism of enantioseparation in these
     arrangements was suggested.,
  DOI = 10.1002/elps.200600063,
  ISSN = 0173-0835,
  Unique-ID = ISI:000242903000017,
  

• J. Petr, S. Gerstmann, and H. Frank, “Determination of some heavy metal cations in molten snow by transient
  isotachophoresis/capillary zone electrophoresis,” JOURNAL OF SEPARATION SCIENCE, vol. 29, iss. 14, pp. 2256-2260, 2006.
  [Bibtex]

  @article ISI:000240891500019,
  Author = Petr, Jan and Gerstmann, Silke and Frank, Hartmut,
  Title = Determination of some heavy metal cations in molten snow by transient
     isotachophoresis/capillary zone electrophoresis,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2006,
  Volume = 29,
  Number = 14,
  Pages = 2256-2260,
  Month = SEP,
  Abstract = Online combination of transient ITP and CZE is employed for the
     determination of Cd(II), Pb(II), Cu(II), Ni(II), and Zn(II). Acetic acid
     is used for creating the transient isotachophoretic state.
     a-Hydroxyisobutyric acid and 4-aminopyridine are used as BGEs for the
     separation and indirect UV detection. At optimum conditions, the method
     allows to determine the metals at levels of 40 - 120 mu g/L, about 50
     times more sensitive than conventional CZE. In combination with a
     20-fold evaporative concentration, the method is suitable for
     environmental monitoring of the heavy metals in snow samples.,
  DOI = 10.1002/jssc.200600022,
  ISSN = 1615-9306,
  Unique-ID = ISI:000240891500019,
  

• V. Maier, J. Horakova, J. Petr, D. Drahonovsky, and J. Sevcik, “Using of S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium
  tetrafluoroborate as additive to the background electrolyte in capillary
  electrophoresis,” JOURNAL OF CHROMATOGRAPHY A, vol. 1103, iss. 2, pp. 337-343, 2006.
  [Bibtex]

  @article ISI:000234879600019,
  Author = Maier, V and Horakova, J and Petr, J and Drahonovsky, D and Sevcik, J,
  Title = Using of S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium
     tetrafluoroborate as additive to the background electrolyte in capillary
     electrophoresis,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2006,
  Volume = 1103,
  Number = 2,
  Pages = 337-343,
  Month = JAN 27,
  Abstract = We synthesised and used new type of quaternary ammonium salt
     [S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium tetrafluoroborate] as
     effective additive to acidic background electrolytes. We used this
     quaternary ammonium salt as effective agent for capillary zone
     electrophoresis separation of model mixture of five tricyclic
     antidepressants (amitriptyline, nortriptyline, imipramine, desipramine
     and clomipramine) as model analytes. We observed that addition of
     S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium tetrafluoroborate
     ([HMDP](+) [BF4](-)) to acidic background electrolytes leads to
     suppression of magnitude of electroosmotic flow (EOF) and gradually
     change the direction of the EOF. Baseline separation of five TAs was
     achieved by using of 91.1 mmol L-1 (20 g L-1) of [HMDP](+) [BF4](-)
     in 25 mmol L-1 sodium phosphate pH 2.5, where electroosmotic mobility
     was -11.3 x 10(-9) m(2) V-1 s(-1). We achieved baseline separation of
     five TAs with using of [HMDP](+) [BF4](-) as water solution too. We
     observed that [HMDP](+) [BF4](-) Can be used as buffer additive,
     which offers relatively smaller anodic electroosmotic flow instead of
     cationic surfactants that are mostly used for genarating of anodic
     electroosmotic flow in capillary electrophoresis. (c) 2005 Elsevier B.V.
     All fights reserved.,
  DOI = 10.1016/j.chroma.2005.11.011,
  ISSN = 0021-9673,
  Unique-ID = ISI:000234879600019,
  

2005

• V. Maier, J. Horakova, J. Petr, E. Tesarova, P. Coufal, and J. Sevcik, “Chiral separation of tamsulosin by capillary electrophoresis,” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 39, iss. 3-4, pp. 691-696, 2005.
  [Bibtex]

  @article ISI:000232209700047,
  Author = Maier, V and Horakova, J and Petr, J and Tesarova, E and Coufal, P and
     Sevcik, J,
  Title = Chiral separation of tamsulosin by capillary electrophoresis,
  Journal = JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,
  Year = 2005,
  Volume = 39,
  Number = 3-4,
  Pages = 691-696,
  Month = SEP 15,
  Abstract = Enantiomers of (+/-) 5-[2 (R,S)-\[2-(o-ethoxyphenoxy) ethyl]
     amino\ propyl]-2-methoxy-benzenesulfonamide (tamsulosin, drug
     frequently used in the treatment of prostate diseases) were separated by
     capillary electrophoresis (CE). An acidic background electrolyte (BGE)
     with sulfated-beta-cyclodextrin (S-P-CD) was used to create a chiral
     separation environment. Baseline separation of the isomers was achieved
     during 5 min using cathodic electro-osmotic flow (EOF) (countercurrent
     mode). The quantification limits were 5.3 x 10(-6) Mol l(-1) for
     R-isomer and 5.7 x 10(-6) Mol l(-1) for S-isomer. The R.S.D. values of
     peak area were 0.54\% for R-isomer and 0.75\% for S-isomer. The results
     achieved enable determination of 0.5\% of optical impurity. (c) 2005
     Elsevier B.V. All rights reserved.,
  DOI = 10.1016/j.jpba.2005.04.023,
  ISSN = 0731-7085,
  Unique-ID = ISI:000232209700047,
  

• J. Petr, V. Maier, J. Horakova, E. Tesarova, and J. Sevcik, “How to achieve successful chiral separation by capillary electrophoresis,” CHEMICKE LISTY, vol. 99, iss. 3, pp. 190-194, 2005.
  [Bibtex]

  @article ISI:000227483400007,
  Author = Petr, J and Maier, V and Horakova, J and Tesarova, E and Sevcik, J,
  Title = How to achieve successful chiral separation by capillary electrophoresis,
  Journal = CHEMICKE LISTY,
  Year = 2005,
  Volume = 99,
  Number = 3,
  Pages = 190-194,
  Abstract = This work is devoted to enhancing the potential of capillary
     electrophoresis as a separation technique. Improvement of chiral
     resolution of organic bases in an acid electrolyte using sulfated
     P-cyclodextrin as an anionic chiral selector is demonstrated on an
     example of chiral separation of tamsulosin and ephedrine. Better
     resolution of enantiomers; of the bases can be achieved by changing the
     concentration of a chiral selector in compartments of the separation
     system (inlet - capillary - outlet). Migration of the chiral selector in
     the direction opposite to that of the cationic analytes was utilized and
     thus the resolution could be improved. The best resolution was obtained
     if the chiral selector was in the working electrolyte in the capillary
     and outlet vial while the inlet vial contained only the background
     electrolyte. In contrast, if the chiral selector is either only in the
     capillary or in the whole electrophoresis system, a lower resolution was
     obtained though both the systems are most frequently employed in
     practice.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000227483400007,