Petr Bednář

2011

• D. Jirovsky, P. Bednar, R. Myjavcova, Z. Bartosova, J. Skopalova, M. Tvrdonova, and K. Lemr, “Study of electrochemical oxidation of cyanidin glycosides by online
  combination of electrochemistry with electrospray ionization tandem mass
  spectrometry,” MONATSHEFTE FUR CHEMIE, vol. 142, iss. 12, pp. 1211-1217, 2011.
  [Bibtex]

  @article ISI:000297146300002,
  Author = Jirovsky, David and Bednar, Petr and Myjavcova, Renata and Bartosova,
     Zdenka and Skopalova, Jana and Tvrdonova, Michaela and Lemr, Karel,
  Title = Study of electrochemical oxidation of cyanidin glycosides by online
     combination of electrochemistry with electrospray ionization tandem mass
     spectrometry,
  Journal = MONATSHEFTE FUR CHEMIE,
  Year = 2011,
  Volume = 142,
  Number = 12,
  Pages = 1211-1217,
  Month = DEC,
  Abstract = Electrochemistry coupled with mass spectrometry (EC-MS) is a promising
     analytical tool for the online study of oxidation processes of
     anthocyanins. Two cyanidin glycosides, cyanidin-3-galactoside (ideain)
     and cyanidin-3,5-diglucoside (cyanin), were subjected to electrochemical
     oxidation and subsequent online mass spectrometric identification of the
     formed products. Application of relatively low working potentials
     (around 100 mV vs. Pd/H(2)) using a porous graphite coulometric
     electrode yielded detectable oxidation products. As determined by
     hydrodynamic voltammetry, the monoglycosylated analogue undergoes anodic
     oxidation easier than the diglycosylated one. As a first step of the
     electrochemically induced oxidation, incorporation of a hydroxyl group
     was observed for both glycosides. Besides, an oxidative condensation of
     two anthocyanin molecules was observed. The proposed oxidative
     condensation was further confirmed by consecutive fragmentation (i.e.,
     collection of MS(2), MS(3), and MS(4) spectra) in which corresponding
     subsequent losses of the sugar moiety were observed.,
  DOI = 10.1007/s00706-011-0650-z,
  ISSN = 0026-9247,
  Unique-ID = ISI:000297146300002,
  

• B. Papouskova, P. Bednar, K. Hron, J. Stavek, J. Balik, R. Myjavcova, P. Bartak, E. Tomankova, and K. Lemr, “Advanced liquid chromatography/mass spectrometry profiling of
  anthocyanins in relation to set of red wine varieties certified in Czech
  Republic,” JOURNAL OF CHROMATOGRAPHY A, vol. 1218, iss. 42, pp. 7581-7591, 2011.
  [Bibtex]

  @article ISI:000296037900020,
  Author = Papouskova, Barbora and Bednar, Petr and Hron, Karel and Stavek, Jan and
     Balik, Josef and Myjavcova, Renata and Bartak, Petr and Tomankova, Eva
     and Lemr, Karel,
  Title = Advanced liquid chromatography/mass spectrometry profiling of
     anthocyanins in relation to set of red wine varieties certified in Czech
     Republic,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2011,
  Volume = 1218,
  Number = 42,
  Pages = 7581-7591,
  Month = OCT 21,
  Abstract = Hyphenation of micro-liquid and ultra-performance liquid chromatography
     with a hybrid (QqTOF) tandem mass spectrometry was studied from the
     viewpoint of their applicability for monitoring of anthocyanin dyes in
     various cultivars and different vintages of red wine. After appropriate
     optimization both techniques proved to be suitable for this task. UHPLC
     system provided lower LOD and LOQ values as well as higher productivity
     and precision of retention parameters and peak areas with respect to
     micro-LC method. On the other hand, micro-LC method offers significant
     savings of mobile phase, less contamination of ion source of mass
     spectrometer and makes the nebulization process in electrospray much
     easier. In combination with principal component analysis (PCA), the
     method allows to elucidate long-term relations in a complete set of
     certified red wine cultivars grown in South Moravia (certified in Czech
     Republic until year 2005). Among the studied varieties Rubinet exhibit a
     specific anthocyanin profile. Especially interesting is a high content
     of 3-coumaroylglucoside-5-glucosides of malvidin and peonidin. Those
     dyes can be used as markers of artificial color enhancement. Principal
     component analysis applied to data processed with regard to enzymatic
     activity markedly facilitate classification among varieties. This
     approach allows resolution of teinturiers. variety Blaufrankish and
     partial classification of other varieties. (C) 2011 Elsevier B.V. All
     rights reserved.,
  DOI = 10.1016/j.chroma.2011.07.027,
  ISSN = 0021-9673,
  Unique-ID = ISI:000296037900020,
  

• P. Marhol, R. Gazak, P. Bednar, and V. Kren, “Narrow-bore core-shell particles and monolithic columns in the analysis
  of silybin diastereoisomers,” JOURNAL OF SEPARATION SCIENCE, vol. 34, iss. 16-17, SI, pp. 2206-2213, 2011.
  [Bibtex]

  @article ISI:000295189400031,
  Author = Marhol, Petr and Gazak, Radek and Bednar, Petr and Kren, Vladimir,
  Title = Narrow-bore core-shell particles and monolithic columns in the analysis
     of silybin diastereoisomers,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2011,
  Volume = 34,
  Number = 16-17, SI,
  Pages = 2206-2213,
  Month = SEP,
  Abstract = Two chromatographic narrow-bore columns, a novel 2.6 pm particle-packed
     Kinetex (TM) C(18) core-shell (50 x 2.1 mm id) and monolithic Chromolith
     (R) FastGradient RP-18e (50 x 2 mm id), were evaluated for the analysis
     of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin
     under isocratic conditions. The main advantages of the core-shell column
     are markedly higher efficiency (h(min) = 2.8 versus 5.6 for silybin A)
     and better peak symmetry. The Kinetex column exhibits only a slight
     change in the height equivalent of the theoretical plate with a higher
     linear velocity of the mobile phase. The monolithic column shows notably
     higher selectivity in terms of selectivity factor (1.21 versus 1.12) in
     the analysis of critical-pair of diastereoisomers (silybin A and silybin
     B) and enables shorter run duration (approx. twofold) together with
     lower backpressure. The resolution power was found to be comparable, but
     the Kinetex column required a higher pressure of the mobile phase that,
     together with the higher chance of clogging, can be a disadvantage in
     the separation of biological samples. Successful baseline separation of
     silybin diastereoisomers in real pharmaceutical sample on monolithic
     column was accomplished.,
  DOI = 10.1002/jssc.201100309,
  ISSN = 1615-9306,
  Unique-ID = ISI:000295189400031,
  

• A. Uygun, R. Myjavcova, A. G. Yavuz, J. V. Ruppel, K. B. Fields, A. Frankhauser, P. X. Zhang, V. R. Bhethanbotla, and P. Bednar, “Electrochemical Polymerization and Investigation of Properties of
  Poly(5-(4′-aminophenylamino)-10,20-diphenylporphyrin),” ELECTROANALYSIS, vol. 23, iss. 5, SI, pp. 1158-1167, 2011.
  [Bibtex]

  @article ISI:000290487600016,
  Author = Uygun, Aysegul and Myjavcova, Renata and Yavuz, Ayse Gul and Ruppel,
     Joshua V. and Fields, Kimberly B. and Frankhauser, Anita and Zhang, X.
     Peter and Bhethanbotla, Venkat R. and Bednar, Petr,
  Title = Electrochemical Polymerization and Investigation of Properties of
     Poly(5-(4'-aminophenylamino)-10,20-diphenylporphyrin),
  Journal = ELECTROANALYSIS,
  Year = 2011,
  Volume = 23,
  Number = 5, SI,
  Pages = 1158-1167,
  Month = MAY,
  Abstract = The preparation and electropolymerization of a new porphyrin monomer
     with an amino group connecting the porphyrin core and aniline unit were
     studied. Cyclic voltammetry, FTIR spectroscopy, and a tandem
     quadrupole/time of flight mass spectrometry with matrix-assisted laser
     desorption/ionization source (MALDI-QqTOF) were used for the
     characterization of
     poly(5-(4'-aminophenylamino)-10,20-diphenylporphyrin) (PAPADPP). The
     PAPADPP formed a smooth layer on the Indium tin oxide (ITO) electrode
     and grew perpendicular to the electrode surface. An oligonucleotide
     (ODN)-probe was physisorbed onto the PAPADPP film and tested for
     hybridization with complementary and noncomplementary ODN segments.
     Biological recognition was monitored by comparison of the
     electrochemical signals (cyclic voltammogram) from single and double
     strand state oligonucleotides. The morphology of the physisorbed
     ODN-probe and its hybridization were observed on ITO electrodes using
     AFM and SEM.,
  DOI = 10.1002/elan.201000609,
  ISSN = 1040-0397,
  Unique-ID = ISI:000290487600016,
  

2010

• R. Myjavcova, P. Marhol, V. Kren, V. Simanek, J. Ulrichova, I. Palikova, B. Papouskova, K. Lemr, and P. Bednar, “Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using
  chromatographic fractionation followed by microcolumn liquid
  chromatography-mass spectrometry,” JOURNAL OF CHROMATOGRAPHY A, vol. 1217, iss. 51, pp. 7932-7941, 2010.
  [Bibtex]

  @article ISI:000285429700002,
  Author = Myjavcova, Renata and Marhol, Petr and Kren, Vladimir and Simanek, Vilim
     and Ulrichova, Jitka and Palikova, Irena and Papouskova, Barbora and
     Lemr, Karel and Bednar, Petr,
  Title = Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using
     chromatographic fractionation followed by microcolumn liquid
     chromatography-mass spectrometry,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2010,
  Volume = 1217,
  Number = 51,
  Pages = 7932-7941,
  Month = DEC 17,
  Note = 25th International Symposium on Microscale BioSeparations (MSB 2010),
     Prague, CZECH REPUBLIC, MAR 21-25, 2010,
  Abstract = Anthocyanins from the fruit Lonicera caerulea L var. kamtschatica
     (blueberry honeysuckle, Caprifoliaceae) were studied via
     (semi)preparative chromatographic fractionation followed by MS and mu
     LC/MS analysis. The extraction procedure was optimized with respect to
     analytical purposes as well as its potential use for the preparation of
     nutraceuticals. The highest yield of anthocyanins was obtained using
     acidified methanol as the extraction medium. A comparable total
     anthocyanin content was obtained using a mixture of methanol and
     acetone. However, when Lonicera anthocyanins were in contact with
     acetone, a condensation reaction occurred to a large extent and related
     5-methylpyranoanthocyanins were found. The effect of other extraction
     media, including ethanol as a ``green'' solvent, is also discussed.
     The potential of two fractionation procedures for extract purification
     differing in their chromatographic selectivity and scale was studied
     (i.e. using a Sephadex LH-20 gel column and a reversed phase). Fractions
     obtained by both procedures were used for a detailed analysis. MS and mu
     LC/MS(2) methods were used for monitoring anthocyanin and
     5-methylpyranoderivatives content as well as identifying less common and
     more complex dyes (dimer of cyanidin-3-hexoside,
     cyanidin-ethyl-catechin-hexosides, etc.). These more complex dyes are
     most likely formed during fruit treatment. (C) 2010 Elsevier B.V. All
     rights reserved.,
  DOI = 10.1016/j.chroma.2010.05.058,
  ISSN = 0021-9673,
  Unique-ID = ISI:000285429700002,
  

• G. Aad, B. Abbott, J. Abdallah, A. A. Abdelalim, A. Abdesselam, O. Abdinov, B. Abi, M. Abolins, H. Abramowicz, H. Abreu, B. S. Acharya, D. L. Adams, T. N. Addy, J. Adelman, C. Adorisio, P. Adragna, T. Adye, S. Aefsky, J. A. Aguilar-Saavedra, M. Aharrouche, S. P. Ahlen, F. Ahles, A. Ahmad, H. Ahmed, M. Ahsan, G. Aielli, T. Akdogan, T. P. A. Akesson, G. Akimoto, A. V. Akimov, A. Aktas, M. S. Alam, M. A. Alam, J. Albert, S. Albrand, M. Aleksa, I. N. Aleksandrov, F. Alessandria, C. Alexa, G. Alexander, G. Alexandre, T. Alexopoulos, M. Alhroob, M. Aliev, G. Alimonti, J. Alison, M. Aliyev, P. P. Allport, S. E. Allwood-Spiers, J. Almond, A. Aloisio, R. Alon, A. Alonso, M. G. Alviggi, K. Amako, C. Amelung, V. V. Ammosov, A. Amorim, G. Amoros, N. Amram, C. Anastopoulos, T. Andeen, C. F. Anders, K. J. Anderson, A. Andreazza, V. Andrei, X. S. Anduaga, A. Angerami, F. Anghinolfi, N. Anjos, A. Antonaki, M. Antonelli, S. Antonelli, B. Antunovic, F. Anulli, S. Aoun, G. Arabidze, I. Aracena, Y. Arai, A. T. H. Arce, J. P. Archambault, S. Arfaoui, J. -F. Arguin, T. Argyropoulos, E. Arik, M. Arik, A. J. Armbruster, O. Arnaez, C. Arnault, A. Artamonov, D. Arutinov, M. Asai, S. Asai, R. Asfandiyarov, S. Ask, B. Asman, D. Asner, L. Asquith, K. Assamagan, A. Astbury, A. Astvatsatourov, G. Atoian, B. Auerbach, E. Auge, K. Augsten, M. Aurousseau, N. Austin, G. Avolio, R. Avramidou, D. Axen, C. Ay, G. Azuelos, Y. Azuma, M. A. Baak, G. Baccaglioni, C. Bacci, A. Bach, H. Bachacou, K. Bachas, M. Backes, E. Badescu, P. Bagnaia, Y. Bai, D. C. Bailey, T. Bain, J. T. Baines, O. K. Baker, M. D. Baker, B. D. S. F. Pedrosa, E. Banas, P. Banerjee, S. Banerjee, D. Banfi, A. Bangert, V. Bansal, S. P. Baranov, S. Baranov, A. Barashkou, T. Barber, E. L. Barberio, D. Barberis, M. Barbero, D. Y. Bardin, T. Barillari, M. Barisonzi, T. Barklow, N. Barlow, B. M. Barnett, R. M. Barnett, S. Baron, A. Baroncelli, A. J. Barr, F. Barreiro, B. G. J. da Costa, P. Barrillon, N. Barros, R. Bartoldus, D. Bartsch, J. Bastos, R. L. Bates, S. Bathe, L. Batkova, J. R. Batley, A. Battaglia, M. Battistin, F. Bauer, H. S. Bawa, M. Bazalova, B. Beare, T. Beau, P. H. Beauchemin, R. Beccherle, N. Becerici, P. Bechtle, G. A. Beck, H. P. Beck, M. Beckingham, K. H. Becks, I. Bedajanek, A. J. Beddall, A. Beddall, P. Bednar, V. A. Bednyakov, C. Bee, M. Begel, B. S. Harpaz, P. K. Behera, M. Beimforde, C. Belanger-Champagne, P. J. Bell, W. H. Bell, G. Bella, L. Bellagamba, F. Bellina, M. Bellomo, A. Belloni, K. Belotskiy, O. Beltramello, S. Ben Ami, O. Benary, D. Benchekroun, M. Bendel, B. H. Benedict, N. Benekos, Y. Benhammou, G. P. Benincasa, D. P. Benjamin, M. Benoit, J. R. Bensinger, K. Benslama, S. Bentvelsen, M. Beretta, D. Berge, B. E. Kuutmann, N. Berger, F. Berghaus, E. Berglund, J. Beringer, K. Bernardet, P. Bernat, R. Bernhard, C. Bernius, T. Berry, A. Bertin, N. Besson, S. Bethke, R. M. Bianchi, M. Bianco, O. Biebel, J. Biesiada, M. Biglietti, H. Bilokon, M. Bindi, S. Binet, A. Bingul, C. Bini, C. Biscarat, U. Bitenc, K. M. Black, R. E. Blair, J. -B. Blanchard, G. Blanchot, C. Blocker, J. Blocki, A. Blondel, W. Blum, U. Blumenschein, G. J. Bobbink, A. Bocci, M. Boehler, J. Boek, N. Boelaert, S. Boeser, J. A. Bogaerts, A. Bogouch, C. Bohm, J. Bohm, V. Boisvert, T. Bold, V. Boldea, A. Boldyrev, V. G. Bondarenko, M. Bondioli, M. Boonekamp, J. R. A. Booth, S. Bordoni, C. Borer, A. Borisov, G. Borissov, I. Borjanovic, S. Borroni, K. Bos, D. Boscherini, M. Bosman, M. Bosteels, H. Boterenbrood, J. Bouchami, J. Boudreau, E. V. Bouhova-Thacker, C. Boulahouache, C. Bourdarios, J. Boyd, I. R. Boyko, I. Bozovic-Jelisavcic, J. Bracinik, A. Braem, P. Branchini, G. W. Brandenburg, A. Brandt, G. Brandt, O. Brandt, U. Bratzler, B. Brau, J. E. Brau, H. M. Braun, B. Brelier, J. Bremer, R. Brenner, S. Bressler, D. Breton, N. D. Brett, D. Britton, F. M. Brochu, I. Brock, R. Brock, T. J. Brodbeck, E. Brodet, F. Broggi, C. Bromberg, G. Brooijmans, W. K. Brooks, G. Brown, E. Brubaker, B. P. A. de Renstrom, D. Bruncko, R. Bruneliere, S. Brunet, A. Bruni, G. Bruni, M. Bruschi, T. Buanes, F. Bucci, J. Buchanan, P. Buchholz, A. G. Buckley, I. A. Budagov, B. Budick, V. Buescher, L. Bugge, O. Bulekov, M. Bunse, T. Buran, H. Burckhart, S. Burdin, T. Burgess, S. Burke, E. Busato, P. Bussey, C. P. Buszello, F. Butin, B. Butler, J. M. Butler, C. M. Buttar, J. M. Butterworth, T. Byatt, J. Caballero, S. Cabrera Urban, D. Caforio, O. Cakir, P. Calafiura, G. Calderini, P. Calfayan, R. Calkins, L. P. Caloba, R. Caloi, D. Calvet, P. Camarri, M. Cambiaghi, D. Cameron, F. Campabadal Segura, S. Campana, M. Campanelli, V. Canale, F. Canelli, A. Canepa, J. Cantero, L. Capasso, C. M. D. M. Garrido, I. Caprini, M. Caprini, M. Capua, R. Caputo, D. Caracinha, C. Caramarcu, R. Cardarelli, T. Carli, G. Carlino, L. Carminati, B. Caron, S. Caron, C. G. D. Montoya, C. S. Montero, A. A. Carter, J. R. Carter, J. Carvalho, D. Casadei, M. P. Casado, M. Cascella, C. Caso, C. A. M. Hernadez, E. Castaneda-Miranda, V. Castillo Gimenez, N. Castro, G. Cataldi, A. Catinaccio, J. R. Catmore, A. Cattai, G. Cattani, S. Caughron, D. Cauz, P. Cavalleri, D. Cavalli, M. Cavalli-Sforza, V. Cavasinni, F. Ceradini, A. S. Cerqueira, A. Cerri, L. Cerrito, F. Cerutti, S. A. Cetin, F. Cevenini, A. Chafaq, D. Chakraborty, K. Chan, J. D. Chapman, J. W. Chapman, E. Chareyre, D. G. Charlton, V. Chavda, S. Cheatham, S. Chekanov, S. V. Chekulaev, G. A. Chelkov, H. Chen, S. Chen, T. Chen, X. Chen, S. Cheng, A. Cheplakov, V. F. Chepurnov, C. R. El Moursli, V. Tcherniatine, D. Chesneanu, E. Cheu, S. L. Cheung, L. Chevalier, F. Chevallier, V. Chiarella, G. Chiefari, L. Chikovani, J. T. Childers, A. Chilingarov, G. Chiodini, M. Chizhov, G. Choudalakis, S. Chouridou, D. Chren, I. A. Christidi, A. Christov, D. Chromek-Burckhart, M. L. Chu, J. Chudoba, G. Ciapetti, A. K. Ciftci, R. Ciftci, D. Cinca, V. Cindro, M. D. Ciobotaru, C. Ciocca, A. Ciocio, M. Cirilli, M. Citterio, A. Clark, W. Cleland, J. C. Clemens, B. Clement, C. Clement, D. Clements, Y. Coadou, M. Cobal, A. Coccaro, J. Cochran, S. Coelli, J. Coggeshall, E. Cogneras, C. D. Cojocaru, J. Colas, B. Cole, A. P. Colijn, C. Collard, N. J. Collins, C. Collins-Tooth, J. Collot, G. Colon, R. Coluccia, P. Conde Muino, E. Coniavitis, M. Consonni, S. Constantinescu, C. Conta, F. Conventi, J. Cook, M. Cooke, B. D. Cooper, A. M. Cooper-Sarkar, N. J. Cooper-Smith, K. Copic, T. Cornelissen, M. Corradi, F. Corriveau, A. Corso-Radu, A. Cortes-Gonzalez, G. Cortiana, G. Costa, M. J. Costa, D. Costanzo, T. Costin, D. Cote, R. Coura Torres, L. Courneyea, G. Cowan, C. Cowden, B. E. Cox, K. Cranmer, J. Cranshaw, M. Cristinziani, G. Crosetti, R. Crupi, S. Crepe-Renaudin, C. C. Almenar, C. T. Donszelmann, M. Curatolo, C. J. Curtis, P. Cwetanski, Z. Czyczula, S. D’Auria, M. D’Onofrio, A. D’Orazio, P. V. M. Da Silva, C. Da Via, W. Dabrowski, T. Dai, C. Dallapiccola, S. J. Dallison, C. H. Daly, M. Dam, H. O. Danielsson, D. Dannheim, V. Dao, G. Darbo, G. L. Darlea, W. Davey, T. Davidek, N. Davidson, R. Davidson, A. R. Davison, I. Dawson, J. W. Dawson, R. K. Daya, K. De, R. de Asmundis, S. De Castro, D. C. F. P. E. Salgado, S. De Cecco, J. de Graat, N. De Groot, P. de Jong, E. De La Cruz-Burelo, C. De La Taille, L. De Mora, D. O. M. Branco, D. De Pedis, A. De Salvo, U. De Sanctis, A. De Santo, D. V. J. B. De Regie, G. De Zorzi, S. Dean, H. Deberg, G. Dedes, D. V. Dedovich, P. O. Defay, J. Degenhardt, M. Dehchar, C. Del Papa, J. Del Peso, T. Del Prete, A. Dell’Acqua, L. Dell’Asta, M. Della Pietra, D. della Volpe, M. Delmastro, N. Delruelle, P. A. Delsart, C. Deluca, S. Demers, M. Demichev, B. Demirkoz, J. Deng, W. Deng, S. P. Denisov, C. Dennis, J. E. Derkaoui, F. Derue, P. Dervan, K. Desch, P. O. Deviveiros, A. Dewhurst, B. DeWilde, S. Dhaliwal, R. Dhullipudi, A. Di Ciaccio, L. Di Ciaccio, A. Di Domenico, A. Di Girolamo, B. Di Girolamo, S. Di Luise, A. Di Mattia, R. Di Nardo, A. Di Simone, R. Di Sipio, M. A. Diaz, F. 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Epp, A. Eppig, V. S. Epshteyn, A. Ereditato, D. Eriksson, I. Ermoline, J. Ernst, M. Ernst, J. Ernwein, D. Errede, S. Errede, E. Ertel, M. Escalier, C. Escobar, X. Espinal Curull, B. Esposito, F. Etienne, A. I. Etienvre, E. Etzion, H. Evans, L. Fabbri, C. Fabre, P. Faccioli, K. Facius, R. M. Fakhrutdinov, S. Falciano, A. C. Falou, Y. Fang, M. Fanti, A. Farbin, A. Farilla, J. Farley, T. Farooque, S. M. Farrington, P. Farthouat, F. Fassi, P. Fassnacht, D. Fassouliotis, B. Fatholahzadeh, L. Fayard, F. Fayette, R. Febbraro, P. Federic, O. L. Fedin, I. Fedorko, W. Fedorko, L. Feligioni, C. U. Felzmann, C. Feng, E. J. Feng, A. B. Fenyuk, J. Ferencei, J. Ferland, B. Fernandes, W. Fernando, S. Ferrag, J. Ferrando, A. Ferrari, P. Ferrari, R. Ferrari, A. Ferrer, M. L. Ferrer, D. Ferrere, C. Ferretti, M. Fiascaris, F. Fiedler, A. Filipcic, A. Filippas, F. Filthaut, M. Fincke-Keeler, M. C. N. Fiolhais, L. Fiorini, A. Firan, G. Fischer, M. J. Fisher, M. Flechl, I. Fleck, J. Fleckner, P. 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  @article ISI:000285200000011,
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     Zutshi, V. and ATLAS Collaboration,
  Title = Readiness of the ATLAS liquid argon calorimeter for LHC collisions,
  Journal = EUROPEAN PHYSICAL JOURNAL C,
  Year = 2010,
  Volume = 70,
  Number = 3,
  Pages = 723-753,
  Month = DEC,
  Abstract = The ATLAS liquid argon calorimeter has been operating continuously since
     August 2006. At this time, only part of the calorimeter was readout, but
     since the beginning of 2008, all calorimeter cells have been connected
     to the ATLAS readout system in preparation for LHC collisions. This
     paper gives an overview of the liquid argon calorimeter performance
     measured in situ with random triggers, calibration data, cosmic muons,
     and LHC beam splash events. Results on the detector operation, timing
     performance, electronics noise, and gain stability are presented. High
     energy deposits from radiative cosmic muons and beam splash events allow
     to check the intrinsic constant term of the energy resolution. The
     uniformity of the electromagnetic barrel calorimeter response along eta
     (averaged over phi) is measured at the percent level using minimum
     ionizing cosmic muons. Finally, studies of electromagnetic showers from
     radiative muons have been used to cross-check the Monte Carlo
     simulation. The performance results obtained using the ATLAS readout,
     data acquisition, and reconstruction software indicate that the liquid
     argon calorimeter is well-prepared for collisions at the dawn of the LHC
     era.,
  DOI = 10.1140/epjc/s10052-010-1354-y,
  ISSN = 1434-6044,
  Unique-ID = ISI:000285200000011,
  

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     and Zmouchko, V. V. and Zobernig, G. and Zoccoli, A. and zur Nedden, M.
     and Zutshi, V. and ATLAS Collaboration,
  Title = Drift Time Measurement in the ATLAS Liquid Argon Electromagnetic
     Calorimeter using Cosmic Muons,
  Journal = EUROPEAN PHYSICAL JOURNAL C,
  Year = 2010,
  Volume = 70,
  Number = 3,
  Pages = 755-785,
  Month = DEC,
  Abstract = The ionization signals in the liquid argon of the ATLAS electromagnetic
     calorimeter are studied in detail using cosmic muons. In particular, the
     drift time of the ionization electrons is measured and used to assess
     the intrinsic uniformity of the calorimeter gaps and estimate its impact
     on the constant term of the energy resolution. The drift times of
     electrons in the cells of the second layer of the calorimeter are
     uniform at the level of 1.3\% in the barrel and 2.8\% in the endcaps.
     This leads to an estimated contribution to the constant term of
     (0.29(-0.04)(+0.05))\% in the barrel and (0.54(-0.04)(+0.06))\% in the
     endcaps. The same data are used to measure the drift velocity of
     ionization electrons in liquid argon, which is found to be 4.61 +/- 0.07
     mm/mu s at 88.5 K and 1 kV/mm.,
  DOI = 10.1140/epjc/s10052-010-1403-6,
  ISSN = 1434-6044,
  Unique-ID = ISI:000285200000012,
  

• L. Hartmanova, V. Ranc, B. Papouskova, P. Bednar, V. Havlicek, and K. Lemr, “Fast profiling of anthocyanins in wine by desorption nano-electrospray
  ionization mass spectrometry,” JOURNAL OF CHROMATOGRAPHY A, vol. 1217, iss. 25, SI, pp. 4223-4228, 2010.
  [Bibtex]

  @article ISI:000278779000031,
  Author = Hartmanova, Lucie and Ranc, Vaclav and Papouskova, Barbora and Bednar,
     Petr and Havlicek, Vladimir and Lemr, Karel,
  Title = Fast profiling of anthocyanins in wine by desorption nano-electrospray
     ionization mass spectrometry,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2010,
  Volume = 1217,
  Number = 25, SI,
  Pages = 4223-4228,
  Month = JUN 18,
  Abstract = Desorption electrospray ionization (DESI) mass spectrometry appears to
     be a useful technique applicable in different areas (e.g. analysis of
     pharmaceuticals, identification of biologically active compounds in
     tissues, imaging mass spectrometry). Its modification termed desorption
     nano-electrospray (nano-DESI) was tested for analysis of anthocyanins.
     Acidifying of samples and acidic spray liquid (methanol:water = 75:25
     with 0.2\% HCOOH) were essential for obtaining good quality spectra.
     Profiles of main anthocyanins in wine samples, two vintages (2005 and
     2007) of three cultivars (Alibernet, Neronet and Rubinet), were
     successfully acquired. They were in agreement with results of LC/MS
     experiments (anthocyanins isolated by solid phase extraction were
     separated by mu-HPLC with gradient elution and detected by ESI-MS).
     Nano-DESI-MS data also allowed to determine ratio of two cultivars
     (Neronet and Rubinet) in their mixture and to detect coloring of wine by
     tenturier or elderberry extract. Detection of main anthocyanins in
     slices of wine grape, chokeberries and elderberries or in a wine stain
     on cotton fabric is also presented. (C) 2010 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.chroma.2010.03.018,
  ISSN = 0021-9673,
  Unique-ID = ISI:000278779000031,
  

• I. Palikova, J. Vostalova, A. Zdarilova, A. Svobodova, P. Kosina, R. Vecera, D. Stejskal, J. Proskova, J. Hrbac, P. Bednar, V. Maier, D. Cernochova, V. Simanek, and J. Ulrichova, “Long-Term Effects of Three Commercial Cranberry Products on the
  Antioxidative Status in Rats: A Pilot Study,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 58, iss. 3, pp. 1672-1678, 2010.
  [Bibtex]

  @article ISI:000274269000041,
  Author = Palikova, Irena and Vostalova, Jitka and Zdarilova, Adela and Svobodova,
     Alena and Kosina, Pavel and Vecera, Rostislav and Stejskal, David and
     Proskova, Jitka and Hrbac, Jan and Bednar, Petr and Maier, Vitezslav and
     Cernochova, Drahomira and Simanek, Vilim and Ulrichova, Jitka,
  Title = Long-Term Effects of Three Commercial Cranberry Products on the
     Antioxidative Status in Rats: A Pilot Study,
  Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
  Year = 2010,
  Volume = 58,
  Number = 3,
  Pages = 1672-1678,
  Month = FEB 10,
  Abstract = Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are
     widely used for their antiadherence and antioxidative properties. Little
     is known however about their effects on clinical chemistry markers after
     long-term consumption. This study was conducted to evaluate the effect
     of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and
     PACRAN on the antioxidative status of rodents, divided into three
     experimental groups. The products were given as dietary admixtures (1500
     mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups
     2-4) and a control Group 1 which received only stock feed. There were no
     significant cranberry treatment-related effects on oxidative stress
     parameters, catalase, glutathione peroxidase, glutathione reductase,
     glutathione transferase, superoxide dismutase, total antioxidant
     capacity, thiobarbituric acid reactive substances, advanced oxidation
     protein products, total SH-groups, or any other measured clinical
     chemistry markers. Hematological parameters, body weight, and food
     consumption were also unaffected by intake of cranberries. Only liver
     glutathione reductase activity and glutathione levels were significantly
     lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was
     significantly decreased in Group 2. No gross pathology, effects on organ
     weights, or histopathology were observed. No genotoxicity was found, and
     total cytochrome P450 level in liver was unaffected in all groups. The
     levels of hippuric acid and several phenolic acids were significantly
     increased in plasma and urine in Groups 2-4. The concentration of
     anthocyanins was under the detection threshold. The dietary addition of
     cranberry powders for 14 weeks was well tolerated, but it did not
     improve the antioxidative status in rats.,
  DOI = 10.1021/jf903710y,
  ISSN = 0021-8561,
  Unique-ID = ISI:000274269000041,
  

2009

• M. Korhonova, K. Hron, D. Klimcikova, L. Mueller, P. Bednar, and P. Bartak, “Coffee aroma-Statistical analysis of compositional data,” TALANTA, vol. 80, iss. 2, pp. 710-715, 2009.
  [Bibtex]

  @article ISI:000271701900045,
  Author = Korhonova, M. and Hron, K. and Klimcikova, D. and Mueller, L. and
     Bednar, P. and Bartak, P.,
  Title = Coffee aroma-Statistical analysis of compositional data,
  Journal = TALANTA,
  Year = 2009,
  Volume = 80,
  Number = 2,
  Pages = 710-715,
  Month = DEC 15,
  Abstract = Solid-phase microextraction in headspace mode coupled with gas
     chromatography-mass spectrometry was applied to the determination of
     volatile compounds in 30 commercially available coffee samples. In order
     to differentiate and characterize Arabica and Robusta coffee, six major
     volatile compounds (acetic acid, 2-methylpyrazine, furfural, 2-furfuryl
     alcohol, 2,6-dimethylpyrazine, 5-methylfurfural) were chosen as the most
     relevant markers. Cluster analysis and principal component analysis
     (PCA) were applied to the raw chromatographic data and data processed by
     centred logratio transformation. (C) 2009 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.talanta.2009.07.054,
  ISSN = 0039-9140,
  Unique-ID = ISI:000271701900045,
  

• B. Papouskova, P. Bednar, J. Styskala, J. Hlavac, P. Bartak, and K. Lemr, “Mass spectrometry as a tool for characterization of
  N,N-dialkylaminoethane-2-thiols – precursors and degradation products of
  chemical warfare agents,” JOURNAL OF MASS SPECTROMETRY, vol. 44, iss. 11, pp. 1604-1612, 2009.
  [Bibtex]

  @article ISI:000272150300008,
  Author = Papouskova, Barbora and Bednar, Petr and Styskala, Jakub and Hlavac, Jan
     and Bartak, Petr and Lemr, Karel,
  Title = Mass spectrometry as a tool for characterization of
     N,N-dialkylaminoethane-2-thiols - precursors and degradation products of
     chemical warfare agents,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2009,
  Volume = 44,
  Number = 11,
  Pages = 1604-1612,
  Month = NOV,
  Abstract = N,N-dialkylaminoethane-2-thiols belong to the group of precursors and
     degradation products of chemical warfare agents (CWAs). These compounds
     were analyzed by means of electrospray ionization-multiple stage mass
     spectrometry (ion trap) and proposed fragments were confirmed by
     accurate mass measurement using a QqTOF system. The fragmentation
     pathways of studied compounds and the products of oxidation (formation
     of -S-S- linkage) were described. Some minor interesting processes, such
     as rearrangement of SH group, were observed and proved. A new mu LC/MS
     method, based on ion-pairing chromatography, was developed.
     Trifluoroacetic acid was employed as an ion-pairing agent to increase
     the low retention of compounds of interest in the reverse-phase system.
     The technique was compared with the UPLC/MS method, allowing fast
     analysis of all the studied thiols as well as an explorative control of
     originated disulfides. Copyright (C) 2009 John Wiley \& Sons, Ltd.,
  DOI = 10.1002/jms.1674,
  ISSN = 1076-5174,
  Unique-ID = ISI:000272150300008,
  

• B. Uhnakova, A. Petrickova, D. Biedermann, L. Homolka, V. Vejvoda, P. Bednar, B. Papouskova, M. Sulc, and L. Martinkova, “Biodegradation of brominated aromatics by cultures and laccase of
  Trametes versicolor,” CHEMOSPHERE, vol. 76, iss. 6, pp. 826-832, 2009.
  [Bibtex]

  @article ISI:000269243900015,
  Author = Uhnakova, B. and Petrickova, A. and Biedermann, D. and Homolka, L. and
     Vejvoda, V. and Bednar, P. and Papouskova, B. and Sulc, M. and
     Martinkova, L.,
  Title = Biodegradation of brominated aromatics by cultures and laccase of
     Trametes versicolor,
  Journal = CHEMOSPHERE,
  Year = 2009,
  Volume = 76,
  Number = 6,
  Pages = 826-832,
  Month = AUG,
  Abstract = 2-Bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3),
     2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobis phenol
     A (6) (1 mM each) added to growing submerged cultures of Trametes
     versicolor CCBAS 612 were eliminated by 65-85\% from the culture medium
     within 4 d. Extracellular laccase activity in the culture medium was
     influenced by the type of brominated compound added. Maximum level of
     laccase (63 U L(-1)) was found in the culture with 2-bromophenol.
     Tetrabromobisphenol A was degraded by a commercial laccase from Trametes
     versicolor in absence of any oxidation mediator, hydroxylated
     dibrominated compounds being detected as soluble reaction products by
     LC/MS. A significant degradation of brominated phenols by laccase was
     achieved only in the presence of ABTS structural characterization of
     major products suggesting reaction between bromophenol and ABTS
     radicals. (C) 2009 Elsevier Ltd. All rights reserved.,
  DOI = 10.1016/j.chemosphere.2009.04.016,
  ISSN = 0045-6535,
  Unique-ID = ISI:000269243900015,
  

• V. Ranc, V. Havlicek, P. Bednar, and K. Lemr, “Magnesium interference and different efficiencies of diastereoisomeric
  cluster formation in phenylalanine enantiomeric discrimination by the
  kinetic method,” INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, vol. 280, iss. 1-3, pp. 213-217, 2009.
  [Bibtex]

  @article ISI:000263195500034,
  Author = Ranc, V. and Havlicek, V. and Bednar, P. and Lemr, K.,
  Title = Magnesium interference and different efficiencies of diastereoisomeric
     cluster formation in phenylalanine enantiomeric discrimination by the
     kinetic method,
  Journal = INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,
  Year = 2009,
  Volume = 280,
  Number = 1-3,
  Pages = 213-217,
  Month = FEB 1,
  Abstract = The kinetic method has been applied for determination of D-Phe/L-Phe
     enantiomeric ratio. Discrimination of enantiomers was inferred from
     product ion mass spectra of trimeric cluster ions containing the analyte
     (L,D-Phe), Cu(2+) as a central metal and L-Trp as a chiral reference
     ligand. Unsatisfactory quantitative results achieved on an ion trap were
     rationalized by high-resolution mass spectrometry. The formation of
     Mg(2+)-containing cluster isobaric to trimeric cluster
     [Cu(L-Trp)(2)Phe](+) was observed. Interference like this was
     identified as a possible reason for deterioration of quantitative
     low-resolution mass spectrometric analyses of real-world samples based
     on the kinetic method. Cation-exchanger was used for easy removal of
     magnesium from a sample and improvement of quantitation.
     Chiral dependence of formation of the Cu(2+)-containing trimeric cluster
     was also observed. Heterochiral diastereoisomeric ions were created less
     effectively. (C) 2008 Elsevier B.V. All rights reserved.,
  DOI = 10.1016/j.ijms.2008.09.009,
  ISSN = 1387-3806,
  Unique-ID = ISI:000263195500034,
  

• V. Ranc, V. Havlicek, P. Bednar, and K. Lemr, “NANOELECTROSPRAY VERSUS ELECTROSPRAY IN CHIRAL ANALYSIS BY THE KINETIC
  METHOD,” COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, vol. 74, iss. 2, pp. 313-322, 2009.
  [Bibtex]

  @article ISI:000265420800005,
  Author = Ranc, Vaclav and Havlicek, Vladimir and Bednar, Petr and Lemr, Karel,
  Title = NANOELECTROSPRAY VERSUS ELECTROSPRAY IN CHIRAL ANALYSIS BY THE KINETIC
     METHOD,
  Journal = COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS,
  Year = 2009,
  Volume = 74,
  Number = 2,
  Pages = 313-322,
  Abstract = Electrospray ionization generates trimeric diastereoisomeric clusters as
     the first important step in chiral analysis by mass spectrometry using
     the Cooks' kinetic method. Cu(2+) and L-tryptophan were used as a
     central metal and as a chiral reference ligand, respectively. The
     comparison of electrospray and nanoelectrospray showed that although the
     electrospray system was generally more robust, the application of
     nanoelectrospray was essential for performing successful analysis in
     some cases, especially for real samples. Basically, no significant
     differences between the ion sources were observed for model samples of
     analytes (isoleucine, ephedrine, phenylalanine) without interfering
     matrix. On the other hand, model samples containing sodium chloride and
     a buffer containing a real sample (drug formulation Mucoseptonex E in
     which D-ephedrine is the active substance) could not be analyzed using
     ESI, whereas nano-ESI gave satisfactory results. An explanation is based
     on the differences of ionization processes occurring in the compared
     sources.,
  DOI = 10.1135/cccc2008201,
  ISSN = 0010-0765,
  Unique-ID = ISI:000265420800005,
  

• J. Styskala, P. Cankar, M. Malon, I. Frysova, P. Bednar, and K. Lemr, “Synthesis of 2-chloro-N,N,N-trialkylethanaminium salts and related
  hydroxy-analogues as analytical standards for monitoring potential
  chemical warfare agent precursors,” ARKIVOC, pp. 45-57, 2009.
  [Bibtex]

  @article ISI:000271148800005,
  Author = Styskala, Jakub and Cankar, Petr and Malon, Michal and Frysova, Iveta
     and Bednar, Petr and Lemr, Karel,
  Title = Synthesis of 2-chloro-N,N,N-trialkylethanaminium salts and related
     hydroxy-analogues as analytical standards for monitoring potential
     chemical warfare agent precursors,
  Journal = ARKIVOC,
  Year = 2009,
  Pages = 45-57,
  Abstract = A set of choline related derivatives of
     2-chloro-N,N,N-trialkylethanaminium salts N-substituted with some simple
     alkyl groups has been prepared and characterized by standard methods.
     Two methodologies based on alkylation were developed. One utilized the
     direct alkylation of 2-chloro-N, N-dialkylethanamines to afford the
     desired aminium salts. Whenever this method failed a three-step
     methodology starting with the alkylation of the 2-(dialkylamino)ethanols
     was used. All these derivatives can be used as analytical standards for
     monitoring potential chemical warfare agent precursors mainly by the
     HPLC-MS methods.,
  ISSN = 1424-6376,
  Unique-ID = ISI:000271148800005,
  

2008

• I. Palikova, J. Heinrich, P. Bednar, P. Marhol, V. Kren, L. Cvak, K. Valentova, F. Ruzicka, V. Hola, M. Kolar, V. Simanek, and J. Ulrichova, “Constituents and Antimicrobial Properties of Blue Honeysuckle: A Novel
  Source for Phenolic Antioxidants,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 56, iss. 24, pp. 11883-11889, 2008.
  [Bibtex]

  @article ISI:000261802400046,
  Author = Palikova, Irena and Heinrich, Jan and Bednar, Petr and Marhol, Petr and
     Kren, Vladimir and Cvak, Ladislav and Valentova, Katerina and Ruzicka,
     Filip and Hola, Veronika and Kolar, Milan and Simanek, Vilim and
     Ulrichova, Jitka,
  Title = Constituents and Antimicrobial Properties of Blue Honeysuckle: A Novel
     Source for Phenolic Antioxidants,
  Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
  Year = 2008,
  Volume = 56,
  Number = 24,
  Pages = 11883-11889,
  Month = DEC 24,
  Abstract = The fruit of Lonicera caerulea L. (blue honeysuckle; Caprifoliaceae) and
     its phenolic fraction were analyzed for nutrients and micronutrients.
     The phenolic fraction was prepared from berries percolated with 0.1\%
     H(3)PO(4) and SPE using Sepabeads SP207. The sugar and lipid content was
     analyzed by HPLC and GC-MS. The total content of anthocyanins was
     determined using the pH differential absorbance method and aliphatic
     acids by capillary electrophoresis. mu LC-MS/MS was used for
     determination of cyanidin-3-glucoside (the predominant anthocyanin),
     3,5-diglucoside, and 3-rutinoside, paeonidin-3-glucoside,
     3,5-diglucoside, and 3-rutinoside, delphinidin-3-glucoside and
     3-rutinoside, pelargonidin-3-glucoside, 3,5-diglucoside, and
     3-rutinoside, quercetin, its 3-glucoside, and 3-rutinoside, epicatechin,
     protocatechuic, gentisic, ellagic, ferulic, caffeic, chlorogenic, and
     coumaric acids. The phenolic fraction displayed Folin-Ciocalteu reagent
     reducing (335 +/- 15 mu g of gallic acid equivalent/mg) and DPPH and
     superoxide scavenging activity (IC(50) 12.1 +/- 0.1 and 115.5 +/- 6.4 mu
     g/mL) and inhibited rat liver microsome peroxidation (IC(50) 160 +/- 20
     mu g/mL). The freeze-dried fruit and its phenolic fraction reduced the
     biofilm formation and adhesion to the artificial surface of Candida
     parapsilosis, Staphylococcus epidermidis, Escherichia coli, Enterococcus
     faecalis, and Streptococcus mutans.,
  DOI = 10.1021/jf8026233,
  ISSN = 0021-8561,
  Unique-ID = ISI:000261802400046,
  

• P. Henklova, R. Vrzal, B. Papouskova, P. Bednar, P. Jancova, E. Anzenbacherova, J. Ulrichova, P. Maurel, P. Pavek, and Z. Dvorak, “SB203580, a pharmacological inhibitor of p38 MAP kinase transduction
  pathway activates ERK and JNK MAP kinases in primary cultures of human
  hepatocytes,” EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 593, iss. 1-3, pp. 16-23, 2008.
  [Bibtex]

  @article ISI:000259508100003,
  Author = Henklova, Pavla and Vrzal, Radim and Papouskova, Barbora and Bednar,
     Petr and Jancova, Petra and Anzenbacherova, Eva and Ulrichova, Jitka and
     Maurel, Patrick and Pavek, Petr and Dvorak, Zdenek,
  Title = SB203580, a pharmacological inhibitor of p38 MAP kinase transduction
     pathway activates ERK and JNK MAP kinases in primary cultures of human
     hepatocytes,
  Journal = EUROPEAN JOURNAL OF PHARMACOLOGY,
  Year = 2008,
  Volume = 593,
  Number = 1-3,
  Pages = 16-23,
  Month = SEP 28,
  Abstract = Mitogen-activated protein kinases (MAPKs) were extensively studied in
     cancer-derived cell lines; however, studies in non-transformed human
     cells are scarce. In the current paper, we studied the effect of
     SB203580, a pharmacological inhibitor of p38 MAPK, on activation and
     inhibition of p38 MAPK transduction partway in primary human hepatocytes
     (in vitro model of differentiated cells) in comparison with several
     tumor cell lines (proliferating non-differentiated in vitro model). In
     addition, we analyzed the effect of SB203580 on extracellular-regulated
     protein kinase (ERK) and c-jun-N-terminal kinase (JNK) pathways both in
     primary human hepatocytes and tumor cell lines employing primary
     antibodies detecting phosphorylated kinases. We show that SB203580
     activates ERK and JNK in primary cultures of human hepatocytes. The
     levels of ERK-P(Thr202/Tyr204),JNK-P(Thr183/Tyr185) and
     c-Jun-P(Ser63/73), a target down-stream protein of JNK, were increased
     by SB203580. In contrast, SB203580 activated ERK but not JNK in HepG2,
     HL-60, Saos-2 and HaCaT human cancer cell lines. We tested, whether the
     effects of SB203580 are due to metabolism. Using liquid
     chromatography/mass spectrometry, we found one minor metabolite in human
     liver microsomes but not in HepG2 cells. These data imply that
     biotransformation could be responsible for the effects of SB203580 in
     human hepatocytes. This study is the first report on the effects of MAPK
     activators (sorbitol, anisomycin, EGF) and MAPK inhibitors in primary
     human hepatocytes. We observed differential effects of these compounds
     in primary human hepatocytes and in cancer cells, implying the cell-type
     specificity and the essential differences between the role and function
     of MAPKs in normal and cancer cells. (C) 2008 Elsevier B.V. All rights
     reserved.,
  DOI = 10.1016/j.ejphar.2008.07.007,
  ISSN = 0014-2999,
  Unique-ID = ISI:000259508100003,
  

• L. Muller, P. Bartak, P. Bednar, I. Frysova, J. Sevcik, and K. Lemr, “Capillary electrophoresis-mass spectrometry – a fast and reliable tool
  for the monitoring of milk adulteration,” ELECTROPHORESIS, vol. 29, iss. 10, pp. 2088-2093, 2008.
  [Bibtex]

  @article ISI:000256773700015,
  Author = Muller, Lukas and Bartak, Petr and Bednar, Petr and Frysova, Iveta and
     Sevcik, Juraj and Lemr, Karel,
  Title = Capillary electrophoresis-mass spectrometry - a fast and reliable tool
     for the monitoring of milk adulteration,
  Journal = ELECTROPHORESIS,
  Year = 2008,
  Volume = 29,
  Number = 10,
  Pages = 2088-2093,
  Month = MAY,
  Abstract = The development of a rapid, simple and accurate analytical method aimed
     at the detection and quantification of bovine milk in either ovine or
     caprine milk samples by means of CEMS analyses of whey proteins with
     high-ionic strength and presence of acidic running buffer is described.
     The high-ionic strength buffer was used in order to minimize the
     problems with the adsorption of the proteins onto the fused-silica
     capillary wall. The acidic running electrolyte, pH 1.9, was used to
     support the production of positive ions in electrospray. Highly linear
     dependences of the ratio of the sum of non-bovine beta-lactoglobulins
     (ovine or caprine) to the total beta-lactoglobulins in milk mixture
     (bovine plus ovine or bovine plus caprine) vs. the volume percentage of
     added bovine milk in ovine (or caprine) milk were obtained. This
     technique allowed the fast and reliable evaluation of milk adulteration.
     The amount of bovine milk added into the ``non-bovine'' ones can be
     well within the concentration range of 5-95\%.,
  DOI = 10.1002/elps.200700794,
  ISSN = 0173-0835,
  Unique-ID = ISI:000256773700015,
  

• Z. Dvorak, R. Vrzal, P. Henklova, P. Jancova, E. Anzenbacheroua, P. Maurel, L. Svecova, P. Pavek, J. Ehrmann, R. Havlik, P. Bednar, K. Lemr, and J. Ulrichova, “JNK inhibitor SP600125 is a partial agonist of human aryl hydrocarbon
  receptor and induces CYP1A1 and CYP1A2 genes in primary human
  hepatocytes,” BIOCHEMICAL PHARMACOLOGY, vol. 75, iss. 2, pp. 580-588, 2008.
  [Bibtex]

  @article ISI:000252842300024,
  Author = Dvorak, Zdenek and Vrzal, Radim and Henklova, Paula and Jancova, Petra
     and Anzenbacheroua, Eva and Maurel, Patrick and Svecova, Lucie and
     Pavek, Petr and Ehrmann, Jiri and Havlik, Roman and Bednar, Petr and
     Lemr, Karel and Ulrichova, Jitka,
  Title = JNK inhibitor SP600125 is a partial agonist of human aryl hydrocarbon
     receptor and induces CYP1A1 and CYP1A2 genes in primary human
     hepatocytes,
  Journal = BIOCHEMICAL PHARMACOLOGY,
  Year = 2008,
  Volume = 75,
  Number = 2,
  Pages = 580-588,
  Month = JAN 15,
  Abstract = SP600125, a specific inhibitor of c-Jun-N-Terminal kinase (JNK), was
     reported as a ligand and antagonist of aryl hydrocarbon receptor (AhR)
     [Joiakim A, Mathieu PA, Palermo C, Gasiewicz TA, Reiners Jr JJ. The
     Jun N terminal kinase inhibitor SP600125 is a ligand and antagonist of
     the aryl hydrocarbon receptor. Drug Metab Dispos 2003;31(11):1279-82].
     Here we show that SP600125 is not an antagonist but a partial agonist of
     human AhR.
     SP600125 significantly induced CYP1A1 and CYP1A2 mRNAs in primary human
     hepatocytes and CYP1A1 mRNA in human hepatoma cells HepG2. This effect
     was abolished by resveratrol, an antagonist of AhR. Consistent with the
     recent report, SP600125 dose-dependently inhibited CYP1A1 and CYPIA2
     genes induction by a prototype AhR ligand 2,3,7,8-tetrachlorodibenzo-p
     -dioxin (TCDD) in human hepatocytes. Moreover, SP600125 displayed
     typical behavior of a partial agonist in HepG2 cells transiently
     transfected with a reporter plasmid containing two inverted repeats of
     the dioxin responsive element or with a plasmid containing 5'-flanking
     region of human CYP1A1 gene. SP600125 transactivated the reporter
     plasmids with EC50 of 0.005 and 1.89 mu M, respectively. On the other
     hand, TCDD-dependent transactivation of the reporter plasmids was
     inhibited by SP600125 with IC50 values of 1.54 and 2.63 mu M,
     respectively. We also tested, whether the effects of SP600125 are due to
     metabolism. Using liquid chromatography/mass spectrometry approach, we
     observed formation of two minor monohydroxylated metabolites of SP600125
     in human hepatocytes, human liver microsomes but not in HepG2 cells.
     These data imply that biotransformation is not responsible for the
     effects of SP600125 on AhR signaling.
     In conclusion, we demonstrate that SP600125 is a partial agonist of
     human AhR, which induces CYP1A genes. (c) 2007 Elsevier Inc. All rights
     reserved.,
  DOI = 10.1016/j.bcp.2007.09.013,
  ISSN = 0006-2952,
  Unique-ID = ISI:000252842300024,
  

• J. Styskala, P. Cankar, M. Soural, P. Bednar, and K. Lemr, “Synthesis of some deuterated dialkylaminoethyls as possible standards
  for the mass spectrometric monitoring of chemical warfare agents,” JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, vol. 51, iss. 1-2, pp. 19-22, 2008.
  [Bibtex]

  @article ISI:000254586300004,
  Author = Styskala, Jakub and Cankar, Petr and Soural, Miroslav and Bednar, Petr
     and Lemr, Karel,
  Title = Synthesis of some deuterated dialkylaminoethyls as possible standards
     for the mass spectrometric monitoring of chemical warfare agents,
  Journal = JOURNAL OF LABELLED COMPOUNDS \& RADIOPHARMACEUTICALS,
  Year = 2008,
  Volume = 51,
  Number = 1-2,
  Pages = 19-22,
  Month = JAN-FEB,
  Abstract = Deuterium-labelled derivatives of 2-(dialkylamino)ethanols,
     2-(dialkylamino)ethyl chlorides and 2-(dialkylamino)ethanethiols have
     been prepared in which completely deuterated N-alkyls (Me, Et, n-Pr,
     iso-Pr) have been introduced as substituents. Such labelled derivatives
     are of great interest in mass spectrometry as standards for monitoring
     chemical warfare agents and their precursors.,
  DOI = 10.1002/jlcr.1464,
  ISSN = 0362-4803,
  Unique-ID = ISI:000254586300004,
  

• Z. Dvorak, R. Vrzal, P. Henklova, P. Jancova, E. Anzenbacherova, P. Maurel, P. Pavek, P. Bednar, and J. Ulrichova, “JNK PHARMACOLOGICAL INHIBITOR SP600125 IS A PARTIAL AGONIST OF HUMAN
  ARYL HYDROCARBON RECEPTOR AND INDUCES CYP1A1 AND CYP1A2 GENES IN PRIMARY
  HUMAN HEPATOCYTES,” DRUG METABOLISM REVIEWS, vol. 40, pp. 147, 2008.
  [Bibtex]

  @article ISI:000265257400128,
  Author = Dvorak, Zdenek and Vrzal, Radim and Henklova, Pavla and Jancova, Petra
     and Anzenbacherova, Eva and Maurel, Patrick and Pavek, Petr and Bednar,
     Petr and Ulrichova, Jitka,
  Title = JNK PHARMACOLOGICAL INHIBITOR SP600125 IS A PARTIAL AGONIST OF HUMAN
     ARYL HYDROCARBON RECEPTOR AND INDUCES CYP1A1 AND CYP1A2 GENES IN PRIMARY
     HUMAN HEPATOCYTES,
  Journal = DRUG METABOLISM REVIEWS,
  Year = 2008,
  Volume = 40,
  Pages = 147,
  Note = 10th European Regional Meeting of the
     International-Society-for-the-Study-of-Xenobiotics, Vienna, AUSTRIA, MAY
     18-21, 2008,
  Organization = Int Soc Study Xenobiot,
  ISSN = 0360-2532,
  Unique-ID = ISI:000265257400128,
  

• V. Ranc, V. Havlicek, P. Bednar, and K. Lemr, “Nano-desorption electrospray and kinetic method in chiral analysis of
  drugs in whole human blood samples,” EUROPEAN JOURNAL OF MASS SPECTROMETRY, vol. 14, iss. 6, pp. 411-417, 2008.
  [Bibtex]

  @article ISI:000262813100009,
  Author = Ranc, Vaclav and Havlicek, Vladimir and Bednar, Petr and Lemr, Karel,
  Title = Nano-desorption electrospray and kinetic method in chiral analysis of
     drugs in whole human blood samples,
  Journal = EUROPEAN JOURNAL OF MASS SPECTROMETRY,
  Year = 2008,
  Volume = 14,
  Number = 6,
  Pages = 411-417,
  Abstract = A home-made nano-desorption electrospray ionization (nano-DESI) device
     and the kinetic method were tested in chiral analysis of model clinical
     samples containing enantiomers of one of three pharmaceutically
     important compounds: dihydroxyphenylalanine (DOPA), ephedrine and
     ibuprofen. The initial evaluation of chiral systems was carried out by
     direct infusion of solution mixtures (analyte/central metal/chiral
     reference ligand) to a standard electrospray ionization (ESI) source.
     Cu(II) was used as a central metal for all. analytes, L-phenylalanine
     was applied as a chiral reference ligand for DOPA, whereas L-tryptophan
     was used for the other two analytes. Then, the ESI source was
     substituted by a nano-DESI source and dried spots of 1 mu L samples of
     whole human blood spiked with individual drugs were successfully
     analyzed without any pre-treatment. Irrespective of a laborious initial
     nano-DESI set-up, the combination of the kinetic method with
     nano-desorption electrospray has, for the first time, been demonstrated
     as a promising tool. for chiral analysis of drugs in blood samples.,
  DOI = 10.1255/ejms.978,
  ISSN = 1469-0667,
  Unique-ID = ISI:000262813100009,
  

• R. Vrzal, P. Henklova, B. Papoukova, P. Bednar, P. Jancova, E. Anzenbacherova, J. Ulrichova, P. Maurel, P. Pavek, and Z. Dvorak, “AN INHIBITOR OF P38 MAP KINASE ACTIVATES ERK AND JNK MAP KINASES IN
  PRIMARY CULTURES OF HUMAN HEPATOCYTES,” DRUG METABOLISM REVIEWS, vol. 40, pp. 146, 2008.
  [Bibtex]

  @article ISI:000265257400127,
  Author = Vrzal, Radim and Henklova, Pavla and Papoukova, Barbora and Bednar, Petr
     and Jancova, Petra and Anzenbacherova, Eva and Ulrichova, Jitka and
     Maurel, Patrick and Pavek, Petr and Dvorak, Zdenek,
  Title = AN INHIBITOR OF P38 MAP KINASE ACTIVATES ERK AND JNK MAP KINASES IN
     PRIMARY CULTURES OF HUMAN HEPATOCYTES,
  Journal = DRUG METABOLISM REVIEWS,
  Year = 2008,
  Volume = 40,
  Pages = 146,
  Note = 10th European Regional Meeting of the
     International-Society-for-the-Study-of-Xenobiotics, Vienna, AUSTRIA, MAY
     18-21, 2008,
  Organization = Int Soc Study Xenobiot,
  ISSN = 0360-2532,
  Unique-ID = ISI:000265257400127,
  

2007

• B. Papouskova, P. Bednar, L. Frysova, J. Styskala, J. Hlavac, P. Bartak, J. Ulrichova, J. Jirkovsky, and K. Lemr, “Mass spectrometric study of selected precursors and degradation products
  of chemical warfare agents,” JOURNAL OF MASS SPECTROMETRY, vol. 42, iss. 12, pp. 1550-1561, 2007.
  [Bibtex]

  @article ISI:000252511900003,
  Author = Papouskova, Barbora and Bednar, Petr and Frysova, Lveta and Styskala,
     Jakub and Hlavac, Jan and Bartak, Petr and Ulrichova, Jitka and
     Jirkovsky, Jaromir and Lemr, Karel,
  Title = Mass spectrometric study of selected precursors and degradation products
     of chemical warfare agents,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2007,
  Volume = 42,
  Number = 12,
  Pages = 1550-1561,
  Month = DEC,
  Note = 25th Informal Meeting on Mass Spectrometry (IMMS), Nyiregyhaza-Sosto,
     HUNGARY, MAY 06-10, 2007,
  Organization = Natl Sci Council, Taiwan; Natl Sun Tat-Sen Univ; Org Prohibition Chem
     Weapons; State Off Nucl Safety; Minist Educ, Youth \& Sports,
  Abstract = Selected precursors and degradation products of chemical warfare agents
     namely N,N-dialkylaminoethane-2-ols, N,N-dialkylaminoethyl-2-chlorides
     and some of related N-quaternary salts were studied by means of
     electrospray ionization-multiple tandem mass spectrometry (ESI-MSn).
     Proposed structures were confirmed with accurate mass measurement.
     General fragmentation patterns of these compounds are discussed in
     detail and suggested processes are confirmed using deuterated standards.
     The typical processes are elimination of alkene, hydrogen chloride, or
     water, respectively. Besides, elimination of ethene from propyl chain
     under specific conditions was observed and unambiguously confirmed using
     exact mass measurement and labelled standard. The potential of mass
     spectrometry to distinguish the positional isomers occurring among the
     studied compounds is reviewed in detail using two different MS
     instruments (i.e. ion trap and hybrid quadrupole-time of flight (Q-TOF)
     analyzer). A new microcolumn liquid chromatography (mu LC)/MSn method
     was designed for the cases where the resolution based solely on
     differences in fragmentation is not sufficient. Low retention of the
     derivatives on reversed phase (RP) was overcome by using addition of
     less typical ion pairing agent (1 mm/l, 3,5-dinitrobenzoic acid) to the
     mobile phase (mixture water: acetonitrile). Copyright (C) 2007 John
     Wiley \& Sons, Ltd.,
  DOI = 10.1002/jms.1319,
  ISSN = 1076-5174,
  Unique-ID = ISI:000252511900003,
  

• J. Lasovsky, J. Hrbac, D. Sichertova, and P. Bednar, “Oxidation and chemiluminescence of catechol by hydrogen peroxide in the
  presence of Co(II) ions and CTAB micelles,” LUMINESCENCE, vol. 22, iss. 5, pp. 501-506, 2007.
  [Bibtex]

  @article ISI:000250706800016,
  Author = Lasovsky, Jan and Hrbac, Jan and Sichertova, Dana and Bednar, Petr,
  Title = Oxidation and chemiluminescence of catechol by hydrogen peroxide in the
     presence of Co(II) ions and CTAB micelles,
  Journal = LUMINESCENCE,
  Year = 2007,
  Volume = 22,
  Number = 5,
  Pages = 501-506,
  Month = SEP-OCT,
  Abstract = The oxidation of catechol in neutral and slightly alkaline aqueous
     solutions (pH 7-9.6) by excess hydrogen peroxide (0.002-0.09 mol/L) in
     the presence of Co(II) (2.10(-7)-2.10(-5) mol/L) is accompanied by
     abrupt formation of red purple colouration, which is subsequently
     decolourized within 1 h. The electron spectra of the reaction mixture
     are characterized by a broad band covering the whole visible range
     (400-700 nm), with maximum at 485 nm. The reaction is initiated by
     catechol oxidation to its semiquinone radical and further to
     1,2-benzoquinone. By nucleophilic addition of hydrogen peroxide into the
     p-position of benzoquinone C=O groups, hydroperoxide intermediates are
     formed, which decompose to hydroxylated 1,4-benzoquinones. It was
     confirmed by MS spectroscopy that monohydroxy-, dihydroxy- and
     tetrahydroxy-1,4-benzoquinone are formed as intermediate products. As
     final products of catechol decomposition, muconic acid, its hydroxy- and
     dihydroxy- derivatives and crotonic acid were identified. In the
     micellar environment of hexaclecyltrimethyl ammonium bromide the
     decomposition rate of catechol is three times faster, due to micellar
     catalysis, and is accompanied by chemiluminescence (CL) emission, with
     maxima at 500 and 640 nm and a quantum yield of 1 x 10(-4). The CL of
     catechol can be further sensitized by a factor of 8 (maximum) with the
     aid of intramicellar energy transfer to fluorescein. Copyright (C) 2007
     John Wiley \& Sons, Ltd.,
  DOI = 10.1002/bio.995,
  ISSN = 1522-7235,
  Unique-ID = ISI:000250706800016,
  

• K. Valentova, D. Stejskal, P. Bednar, J. Vostalova, C. Cihalik, R. Vecerova, D. Koukalova, M. Kolar, R. Reichenbach, L. Sknouril, J. Ulrichova, and V. Simanek, “Biosafety, antioxidant status, and metabolites in urine after
  consumption of dried cranberry juice in healthy women: A pilot
  double-blind placebo-controlled trial,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 55, iss. 8, pp. 3217-3224, 2007.
  [Bibtex]

  @article ISI:000245600300065,
  Author = Valentova, Katerina and Stejskal, David and Bednar, Petr and Vostalova,
     Jitka and Cihalik, Cestmir and Vecerova, Renata and Koukalova, Dagmar
     and Kolar, Milan and Reichenbach, Richard and Sknouril, Lucyna and
     Ulrichova, Jitka and Simanek, Vilim,
  Title = Biosafety, antioxidant status, and metabolites in urine after
     consumption of dried cranberry juice in healthy women: A pilot
     double-blind placebo-controlled trial,
  Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
  Year = 2007,
  Volume = 55,
  Number = 8,
  Pages = 3217-3224,
  Month = APR 18,
  Abstract = This study assessed the effect of an 8 week consumption of dried
     cranberry juice (DCJ) on 65 healthy young women. Basic biochemical and
     hematological parameters, antioxidant status, presence of metabolites in
     urine, and urine ex vivo antiadherence activity were determined
     throughout the trial. A 400 mg amount of DCJ/day had no influence on any
     parameter tested. A 1200 mg amount of DCJ/day resulted in a
     statistically significant decrease in serum levels of advanced oxidation
     protein products. This specific protective effect against oxidative
     damage of proteins is described here for the first time. Urine samples
     had an inhibitory effect on the adhesion of uropathogenic Escherichia
     coli strains, but no increase in urine acidity was noted. Hippuric acid,
     isomers of salicyluric and dihydroxybenzoic acids, and quercetin
     glucuronide were identified as the main metabolites. In conclusion,
     cranberry fruits are effective not only in the prevention of urinary
     tract infection but also for the prevention of oxidative stress.,
  DOI = 10.1021/jf0636014,
  ISSN = 0021-8561,
  Unique-ID = ISI:000245600300065,
  

• V. Ranca, V. Havlicek, P. Bednar, and K. Lemr, “Desorption electrospray: A modern tool for organic surface analysis,” CHEMICKE LISTY, vol. 101, iss. 6, pp. 524-529, 2007.
  [Bibtex]

  @article ISI:000247359700009,
  Author = Ranca, Vaclav and Havlicek, Vladimir and Bednar, Petr and Lemr, Karel,
  Title = Desorption electrospray: A modern tool for organic surface analysis,
  Journal = CHEMICKE LISTY,
  Year = 2007,
  Volume = 101,
  Number = 6,
  Pages = 524-529,
  Abstract = Applicability of mass spectrometry is related to the progress in
     instrumentation including ion sources. Ionization techniques in MS often
     need sample preparation, which increases the analysis time. From this
     point of view, DESI (desorption electrospray ionization) seems to be an
     interesting alternative. Electrospraying of a sample with a liquid leads
     to the production of analyte ions desorbed from the surface-deposited
     sample. A new modification of DESI, where electrospraying is provided by
     nano-ESI, and a new application in chiral analysis have been developed.
     Chiral analysis employs the Cooks kinetic method. Chiral selector is
     sprayed by nano-ESI onto the surface with a deposited chiral analyte.
     Trimerie clusters are formed in the bulk of the ion source. Their
     fragmentation allows chiral discrimination of enantiomers. The power of
     the DESI-based mass spectrometric tool is demonstrated on chiral
     analysis of ephedrine in commercial pharmaceutical Mucoseptonexo E. The
     analysis is performed without any sample preparation. That is why the
     analysis is simple and cost-effective. Calibration for ephedrine was
     performed with D-enantiomer and the racernate (correlation coefficient
     R-2 = 0.996) was obtained.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000247359700009,
  

• J. Styskala, P. Cankar, M. Soural, P. Bednar, and K. Lemr, “Preparation and characterization of some unsymmetrical
  2-(dialkylamino)ethanethiols,” ARKIVOC, iss. Part 15, pp. 171-180, 2007.
  [Bibtex]

  @article ISI:000251574800017,
  Author = Styskala, Jakub and Cankar, Petr and Soural, Miroslav and Bednar, Petr
     and Lemr, Karel,
  Title = Preparation and characterization of some unsymmetrical
     2-(dialkylamino)ethanethiols,
  Journal = ARKIVOC,
  Year = 2007,
  Number = Part 15,
  Pages = 171-180,
  Abstract = The synthesis of some 2-(dialkylamino) ethanethiolsis described. A
     method involving formation of isothiouronium salts from
     2-(dialkylamino)ethyl chlorides by the reaction with thiourea followed
     by the alkaline hydrolysis has been chosen. Prepared compounds can be
     used as the standards for the analytical chemical methods to monitor the
     chemical warfare agents and their precursors or metabolites.,
  ISSN = 1424-6376,
  Unique-ID = ISI:000251574800017,
  

• M. Korhonova, R. Hejdova, P. Bartak, P. Bednar, and L. Cap, “Analysis of markers for characterization of distillates,” CHEMICKE LISTY, vol. 101, iss. 3, pp. 217-219, 2007.
  [Bibtex]

  @article ISI:000245073300005,
  Author = Korhonova, M. and Hejdova, R. and Bartak, P. and Bednar, P. and Cap, L.,
  Title = Analysis of markers for characterization of distillates,
  Journal = CHEMICKE LISTY,
  Year = 2007,
  Volume = 101,
  Number = 3,
  Pages = 217-219,
  Abstract = Fruit distillates, such as apricot-brandy, plum vodka ``Slivovitz'',
     cider-brandy and pear-brandy, originating from domestic production,
     small distilleries and manufacturing production, were analysed by gas
     chromatography with mass spectrometry. Less specific, but universal
     markers present in all types of distillates were selected for first
     experiments: propan-1-ol, propan-2-ol, ethyl acetate,
     2-methylpropan-1-ol, 3-methylbutan-1-ol, 2-methylbutan-1-ol,
     1,1-diethoxyethane, ethyl octanoate, ethyl decanoate, ethyl dodecanoate,
     ethyl tetradecanoate, ethyl palmitate and ethyl stearate. Quantitative
     data were statistically evaluated by factor analysis. Regarding the
     minute specificity of selected markers, unexpectedly good resolution was
     observed in this case. The results of factor analysis indicate a
     possibility of differentiation of products from different fruits.
     Samples of apricot-brandy and plum vodka (drupes) can be differentiated.
     In the same way, distillates from apples and pears (pomes) can be easily
     differentiated. Although, the full resolution of all samples requires
     discovery of other specific markers, the proposed set of
     low-molecular-weight compounds is a promising group of universal markers
     for characterisation of distillates.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000245073300005,
  

• S. Oplustil, D. Chrastina, M. Rulik, P. Bartak, P. Bednar, and L. Cap, “Analysis of phospholipids biomarkers by gas chromatography,” CHEMICKE LISTY, vol. 101, iss. 3, pp. 236-240, 2007.
  [Bibtex]

  @article ISI:000245073300009,
  Author = Oplustil, S. and Chrastina, D. and Rulik, M. and Bartak, P. and Bednar,
     P. and Cap, L.,
  Title = Analysis of phospholipids biomarkers by gas chromatography,
  Journal = CHEMICKE LISTY,
  Year = 2007,
  Volume = 101,
  Number = 3,
  Pages = 236-240,
  Abstract = Most known lipid biomarkers were found by analysis of pure bacterial
     cultures in vitro. The practical impact of a biomarker including
     possible interferences should be tested in extensive research of target
     strains in real ecosystems. Phospholipid fatty acids identified in river
     sediments, activated sludge and waterworks sand filters correspond with
     those proposed previously as biomarkers typical of expected
     microorganisms. Two ether lipids regarded as specific biomarkers of the
     Archaea kingdom were identified in digested sludge from a sewage water
     plant.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000245073300009,
  

2006

• B. Papouskova, P. Bednar, P. Bartak, P. Frycak, J. Sevcik, Z. Stransky, and K. Lemr, “Utilisation of separation methods in the analysis of chemical warfare
  agents,” JOURNAL OF SEPARATION SCIENCE, vol. 29, iss. 11, pp. 1531-1538, 2006.
  [Bibtex]

  @article ISI:000239574100001,
  Author = Papouskova, Barbora and Bednar, Petr and Bartak, Petr and Frycak, Petr
     and Sevcik, Juraj and Stransky, Zdenek and Lemr, Karel,
  Title = Utilisation of separation methods in the analysis of chemical warfare
     agents,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2006,
  Volume = 29,
  Number = 11,
  Pages = 1531-1538,
  Month = JUL,
  Abstract = Chemical warfare agents and their degradation products represent a broad
     group of compounds with different chemical properties (polarity,
     volatility, thermostability, etc.). These chemicals often have to be
     detected and determined in complex matrices and therefore highly
     efficient separation techniques hyphenated to selective and sensitive
     detectors play an indispensable role. This review offers an overview of
     selected papers devoted to the title subject. It cannot be considered as
     a comprehensive literature compilation but should allow the reader to
     obtain an insight into the application of separation techniques in the
     important area of human protection and control of chemical weapons.,
  DOI = 10.1002/jssc.200500432,
  ISSN = 1615-9306,
  Unique-ID = ISI:000239574100001,
  

• K. Lemr, V. Ranc, P. Frycak, P. Bednar, and J. Sevcik, “Chiral analysis by mass spectrometry using the kinetic method in flow
  systems,” JOURNAL OF MASS SPECTROMETRY, vol. 41, iss. 4, pp. 499-506, 2006.
  [Bibtex]

  @article ISI:000237364700008,
  Author = Lemr, K and Ranc, V and Frycak, P and Bednar, P and Sevcik, J,
  Title = Chiral analysis by mass spectrometry using the kinetic method in flow
     systems,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2006,
  Volume = 41,
  Number = 4,
  Pages = 499-506,
  Month = APR,
  Abstract = Chiral analysis is an important task of analytical chemistry. Besides
     separation techniques, mass spectrometry can be applied in this field.
     One mass spectrometric approach is based on Cooks' kinetic method. The
     method was successfully applied in a static system in which the
     concentration of the analyte as well as the chiral selector solution was
     constant during the experiment. The application of the kinetic method in
     dynamic systems (changing concentration of analyte) is presented. Such
     systems allow the speeding up of the analytical process (flow injection
     analysis (FIA)) or the use of the kinetic method for chiral detection
     after liquid chromatographic separation.
     The influence of the concentration of the components of the chiral
     selector solution as well as its flow rate on the recognition of
     enantiomers was evaluated. A new procedure for correction for the
     differences between ratio of enantiomers in the liquid phase and their
     observed ratio in the gas phase is also described. A significant
     improvement in accuracy using this procedure was achieved. Applicability
     of the method was demonstrated in the analysis of amino acids using FIA
     as well as HPLC/MS. After an achiral separation of leucine and
     isoleucine, chiral mass spectrometric detection was successfully used
     for enantiomeric recognition. Copyright (c) 2006 John Wiley \& Sons,
     Ltd.,
  DOI = 10.1002/jms.1008,
  ISSN = 1076-5174,
  Unique-ID = ISI:000237364700008,
  

• D. Friedecky, P. Bednar, M. Prochazka, and T. Adam, “Analysis of intracellular nucleotides by capillary electrophoresis -
  Mass spectrometry,” NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, vol. 25, iss. 9-11, pp. 1233-1236, 2006.
  [Bibtex]

  @article ISI:000242019000050,
  Author = Friedecky, D. and Bednar, P. and Prochazka, M. and Adam, T.,
  Title = Analysis of intracellular nucleotides by capillary electrophoresis -
     Mass spectrometry,
  Journal = NUCLEOSIDES NUCLEOTIDES \& NUCLEIC ACIDS,
  Year = 2006,
  Volume = 25,
  Number = 9-11,
  Pages = 1233-1236,
  Note = 10th Symposium of the
     European-Society-for-the-Study-of-Purine-and-Pyrimidine-Metabolism-in-Ma
     n (ESSPPMM), Prague, CZECH REPUBLIC, JUN 08-11, 2005,
  Organization = European Soc Study Purine \& Pyrimidine Metabolism Man,
  Abstract = A pilot study using capillary electrophoresis with mass spectrometry for
     the analysis of nucleotides in human erythrocytes is presented.
     Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside
     in order to mimic situation in defect of purine metabolism -
     AICA-ribosiduria. Characteristic AICA-ribotides together with normal
     nucleotides were separated by capillary electrophoresis in acetate
     buffer ( 20 mmol/L, pH 4.4) and identified on line by mass spectrometry.,
  DOI = 10.1080/15257770600894667,
  ISSN = 1525-7770,
  Unique-ID = ISI:000242019000050,
  

• V. Ranc, P. Frycak, L. Muller, P. Bednar, and K. Lemr, “Recognition of isomers by mass spectrometry using the kinetic method,” CHEMICKE LISTY, vol. 100, iss. 3, pp. 196-203, 2006.
  [Bibtex]

  @article ISI:000236139700008,
  Author = Ranc, V and Frycak, P and Muller, L and Bednar, P and Lemr, K,
  Title = Recognition of isomers by mass spectrometry using the kinetic method,
  Journal = CHEMICKE LISTY,
  Year = 2006,
  Volume = 100,
  Number = 3,
  Pages = 196-203,
  Abstract = The principle of the Cooks'kinetic method and its application to
     analysis of isomeric mixtures are described. The kinetic method based on
     mass spectrometric measurements is applied to discrimination of isomers
     of various types of compounds (amino acids, peptides, sugars,
     pharmaceuticals, etc.). So far this method has been used in static
     systems (with constant analyte concentrations in the course of
     analytical run). A modification for dynamic systems (flow injection
     analysis, HPLC) is presented. The accuracy of determination in both
     static and dynamic systems can worsen due to different ratios of the
     isomers in liquid and gas phases. A procedure for correction of this
     phenomenon is also demonstrated.,
  ISSN = 0009-2770,
  Unique-ID = ISI:000236139700008,
  

2005

• L. Muller, P. Bednar, P. Bartak, K. Lemr, and J. Sevcik, “Estimation of partition coefficients by MEKC – Part 2: Anthocyanins,” JOURNAL OF SEPARATION SCIENCE, vol. 28, iss. 12, pp. 1285-1290, 2005.
  [Bibtex]

  @article ISI:000231513900005,
  Author = Muller, L and Bednar, P and Bartak, P and Lemr, K and Sevcik, J,
  Title = Estimation of partition coefficients by MEKC - Part 2: Anthocyanins,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2005,
  Volume = 28,
  Number = 12,
  Pages = 1285-1290,
  Month = AUG,
  Note = Symposium on Advances in Chromatography and Electrophoresis and
     Chiranal, Palacky Univ, Fac Phys Culture, Olomouc, CZECH REPUBLIC, FEB
     07-10, 2005,
  Organization = Palacky Univ, Fac Sci Dept Analyt Chem; Czech Chem Soc, Chromatog \&
     Electrophoresis Grp,
  Abstract = The octanol-water partition coefficients of seven anthocyanins (in
     neutral form, pH = 7.0) were measured by means of MEKC. A new iterative
     method for the estimation of migration time of electroosmotic flow (EOF)
     and micelles in MEKC is presented. This calculation is based on
     Gauss-Newton linearization of the dependence between migration indices
     and migration times of a set of suitable standards and the application
     of an advanced statistical evaluation procedure. The values of partition
     coefficients obtained with the aid of an iterative process are compared
     with values obtained on use of EOF and micelle markers.,
  DOI = 10.1002/jssc.200500072,
  ISSN = 1615-9306,
  Unique-ID = ISI:000231513900005,
  

• P. Bednar, B. Papouskova, L. Muller, P. Bartak, J. Stavek, P. Pavlousek, and K. Lemr, “Utilization of capillary electrophoresis/mass spectrometry (CE/MSn) for
  the study of anthocyanin dyes,” JOURNAL OF SEPARATION SCIENCE, vol. 28, iss. 12, pp. 1291-1299, 2005.
  [Bibtex]

  @article ISI:000231513900006,
  Author = Bednar, P and Papouskova, B and Muller, L and Bartak, P and Stavek, J
     and Pavlousek, P and Lemr, K,
  Title = Utilization of capillary electrophoresis/mass spectrometry (CE/MSn) for
     the study of anthocyanin dyes,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2005,
  Volume = 28,
  Number = 12,
  Pages = 1291-1299,
  Month = AUG,
  Note = Symposium on Advances in Chromatography and Electrophoresis and
     Chiranal, Palacky Univ, Fac Phys Culture, Olomouc, CZECH REPUBLIC, FEB
     07-10, 2005,
  Organization = Palacky Univ, Fac Sci Dept Analyt Chem; Czech Chem Soc, Chromatog \&
     Electrophoresis Grp,
  Abstract = Hyphenation of capillary electrophoresis with electrospray ionization
     mass spectrometry was utilized for the monitoring of anthocyanins in
     wine and wine musts. CE/MS was performed in two electrolytes: 1) an
     acidic one (chloroacetate-ammonium, pH 2) and 2) a basic one with high
     selectivity towards derivatives containing vicinal hydroxy groups
     (borate-ammonium, pH 9). The setup of MS was optimized and the
     fragmentation of common anthocyanins was studied in detail. Attention
     was also focused on the fragmentation of anthocyanidin skeleton. The
     anthocyanidins substituted with hydroxy groups fragment via a cascade of
     neutral losses of water and carbon monoxide. Fragmentation of
     anthocyanidins containing a methoxy group on their B-ring starts with
     the cleavage of methane and/or methyl radical. The optimized method was
     utilized for the monitoring of changes in anthocyanin profile in red
     wines as well as the process of release of anthocyanins to wine must.,
  DOI = 10.1002/jssc.200500071,
  ISSN = 1615-9306,
  Unique-ID = ISI:000231513900006,
  

2004

• J. Novotna, A. Honzatko, P. Bednar, J. Kopecky, J. Janata, and J. Spizek, “L-3,4-Dihydroxyphenyl alanine-extradiol cleavage is followed by
  intramolecular cyclization in lincomycin biosynthesis,” EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 271, iss. 18, pp. 3678-3683, 2004.
  [Bibtex]

  @article ISI:000223711400007,
  Author = Novotna, J and Honzatko, A and Bednar, P and Kopecky, J and Janata, J
     and Spizek, J,
  Title = L-3,4-Dihydroxyphenyl alanine-extradiol cleavage is followed by
     intramolecular cyclization in lincomycin biosynthesis,
  Journal = EUROPEAN JOURNAL OF BIOCHEMISTRY,
  Year = 2004,
  Volume = 271,
  Number = 18,
  Pages = 3678-3683,
  Month = SEP,
  Abstract = The LmbB1 protein, participating in the biosynthesis of lincomycin, was
     heterologously expressed in Escherichia coli, purified in its active
     form, and characterized as a dimer of identical subunits. Methods for
     purification and analysis of the LmbB1 reaction product were developed.
     Molecular mass and fragmentation pattern of the product revealed by
     capillary electrophoresis-mass spectrometry were in agreement with its
     proposed structure,
     4-(3-carboxy-3-oxo-propenyl)-2,3-dihydro-1H-pyrrole-2-carboxylic acid.
     The LmbB1 is therefore a dioxygenase catalysing the 2,3-extradiol
     cleavage of the L-3,4-dihydroxyphenyl alanine aromatic ring. The final
     LmbB1 reaction product, a unique compound found in biosynthesis of
     lincomycin and expected in anthramycins, arises through subsequent
     cyclization of the primary cleavage product, 2,3-secodopa. A possible
     role of LmbB1 in 2,3-secodopa cyclization and alternative ways of the
     cyclization in the formation of biosynthetically related compounds,
     muscaflavin and stizolobinic acid, are discussed.,
  DOI = 10.1111/j.1432-1033.2004.04308.x,
  ISSN = 0014-2956,
  Unique-ID = ISI:000223711400007,
  

• P. Bartak, P. Bednar, L. Kubacek, M. Lammerhofer, W. Lindner, and Z. Stransky, “Advanced statistical evaluation of the complex formation constants from
  electrophoretic data II – Diastereomeric ion-pairs of
  (R,S)-N-(3,5-dinitrobenzoyl)leucine and tert-butylcarbamoylquinine,” ANALYTICA CHIMICA ACTA, vol. 506, iss. 1, pp. 105-113, 2004.
  [Bibtex]

  @article ISI:000189102900012,
  Author = Bartak, P and Bednar, P and Kubacek, L and Lammerhofer, M and Lindner, W
     and Stransky, Z,
  Title = Advanced statistical evaluation of the complex formation constants from
     electrophoretic data II - Diastereomeric ion-pairs of
     (R,S)-N-(3,5-dinitrobenzoyl)leucine and tert-butylcarbamoylquinine,
  Journal = ANALYTICA CHIMICA ACTA,
  Year = 2004,
  Volume = 506,
  Number = 1,
  Pages = 105-113,
  Month = MAR 17,
  Abstract = Intermolecular association and ion-pair formation, respectively, between
     a cationic chiral selector, viz. o-9-(tert-butylcarbamoyl) quinine
     (CQN), and the both enantiomers of anionic
     N-(3,5-dinitrobenzoyl)leucine, (R)-DNB-Leu and (S)-DNB-Leu, were
     investigated by affinity capillary electrophoresis (ACE). Thus, binding
     constants of the both diastereomeric ion-pairs, (R) and (S)-DNB-Leu/CQN
     associates, were determined by different experimental setups and
     correction of nonlinear effects. A reciprocal setup was employed for the
     high-affinity (S)-enantiomer, and the experimental mobility data
     obtained for CQN at variable (S)-DNB-Leu concentrations in the
     background electrolyte were linearized and evaluated by advanced
     statistical model. A binding constant of K-S = 125.11 mol(-1) was
     afforded. The constant for the (R)-enantiomer, which is outside the
     range suitable for direct affinity CE, was obtained from indirect
     affinity CE utilizing the separation of the DNB-Leu racemate at a single
     appropriate CQN concentration in the BGE (resolution method) taking
     advantage of the known constant for the (S)-enantiomer yielding a
     binding constant of K-R = 2.511 mol(-1). Thereby, the so-called
     ``constant time method'' was adopted for the required precise
     measurement of the effective mobilities of the both enantiomers. A
     combined approach of reciprocal affinity CE with racemic DNB-Leu as
     additive and the resolution method confirmed the results. The resulting
     constants evidence excellent enantioselectivity of the
     tert-butylcarbamoyl derivative of the cinchona alkaloid quinine as
     chiral selector for N-(3,5-dinitrobenzoyl) derivatives of amino acids.
     (C) 2003 Elsevier B.V. All rights reserved.,
  DOI = 10.1016/j.aca.2003.10.087,
  ISSN = 0003-2670,
  Unique-ID = ISI:000189102900012,
  

2003

• P. Bednar, A. Tomassi, C. Presutti, M. Pavlikova, K. Lemr, and S. Fanali, “Separation of structurally related anthocyanins by MEKC,” CHROMATOGRAPHIA, vol. 58, iss. 5-6, pp. 283-287, 2003.
  [Bibtex]

  @article ISI:000185393700007,
  Author = Bednar, P and Tomassi, AV and Presutti, C and Pavlikova, M and Lemr, K
     and Fanali, S,
  Title = Separation of structurally related anthocyanins by MEKC,
  Journal = CHROMATOGRAPHIA,
  Year = 2003,
  Volume = 58,
  Number = 5-6,
  Pages = 283-287,
  Month = SEP,
  Abstract = A method based on micellar electrokinetic chromatography has been
     developed for the simultaneous separation of six anthocyanins
     (malvidin-3,5-diglucoside, molvidin-3-glucosicle,
     molvidin-3-galactoside, pelargonidin-3-glucoside,
     cyanidin-3,5-diglucoside, cyanidin-3-galactoside). Optimum selectivity
     was achieved in the buffer 30 mM phosphate + 400 mM borate-TRIS, pH =
     7.0 supported with 50 mM sodium dodecylsulphate. High content of borate
     was essential mainly for the separation of diastereomeric pair
     malvidin-3-glucoside-malvidin-3-galactoside. The calibration
     dependencies exhibit good linearity in the ranges of concentration
     10-100 mug mL(-1) for diglycosylated and 25-100 mug mL(-1) for
     monoglycosylated derivatives (R-2 = 0.9711-0.9989). The optimized method
     was applied to a sample of wine grape skin extract. Malvidin-3-glucoside
     was identified as main anthocyanin colorant in this sample.,
  DOI = 10.1365/s10337-003-0051-5,
  ISSN = 0009-5893,
  Unique-ID = ISI:000185393700007,
  

• J. Styskala, J. Slouka, I. Wiedermannova, and P. Bednar, “Utilization of cleavage of the [1]benzofuro[2,3-e][1,2,4]triazine
  ring for the synthesis of oxygen, nitrogen and sulfur derivatives of
  [1,2,4]triazine [1],” JOURNAL OF HETEROCYCLIC CHEMISTRY, vol. 40, iss. 5, pp. 805-811, 2003.
  [Bibtex]

  @article ISI:000186377000010,
  Author = Styskala, J and Slouka, J and Wiedermannova, I and Bednar, P,
  Title = Utilization of cleavage of the [1]benzofuro[2,3-e][1,2,4]triazine
     ring for the synthesis of oxygen, nitrogen and sulfur derivatives of
     [1,2,4]triazine [1],
  Journal = JOURNAL OF HETEROCYCLIC CHEMISTRY,
  Year = 2003,
  Volume = 40,
  Number = 5,
  Pages = 805-811,
  Month = SEP-OCT,
  Abstract = A series of 6-azacytosines 4a-4k and 5a-5c were prepared by nucleophilic
     cleavage of furan ring of [I]benzofuro[2,3-e][1,2,4]triazine
     derivative 1. Some of them were used for the preparation of derivatives
     of [1,2,4]triazolo[4,3-d][1,2,4]triazine (6a-6d) and
     tetrazolo[1,5-d][1,2,4]triazine (7). The reaction of 1 with hydrogen
     sulfide afforded the corresponding
     6-(2-hydroxyphenyl)-2-phenyl-5-thioxo-4,5-dihydro-1,2,4-triazin-3(2H)-on
     e (8), while with hydrogen selenide
     6-(2-hydroxyphenyl)-2-phenyl-4,5-dihydro-1,2,4-triazin-3(2H)-one (9) was
     formed. The prepared compounds were tested for biological activity.,
  ISSN = 0022-152X,
  Unique-ID = ISI:000186377000010,
  

• P. Kosina, J. Sevcik, M. Modriansky, A. Gavenda, P. Bednar, P. Barktak, D. Walterova, and J. Ulrichova, “High performance liquid chromatography and capillary electrophoresis
  determination of sanguinarine in biological matrices,” JOURNAL OF SEPARATION SCIENCE, vol. 26, iss. 8, pp. 679-685, 2003.
  [Bibtex]

  @article ISI:000183660100007,
  Author = Kosina, P and Sevcik, J and Modriansky, M and Gavenda, A and Bednar, P
     and Barktak, P and Walterova, D and Ulrichova, J,
  Title = High performance liquid chromatography and capillary electrophoresis
     determination of sanguinarine in biological matrices,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2003,
  Volume = 26,
  Number = 8,
  Pages = 679-685,
  Month = JUN,
  Note = Symposium on Advances in Chromatography and Electrophoresis and Chiranal
     2002, OLOMOUC, CZECH REPUBLIC, JUL 24-27, 2002,
  Abstract = HPLC and CE methods were employed to determine the quaternary
     benzo[c]phenanthridine alkaloid sanguinarine,in biological matrices
     (rat hepatocytes, human gingival fibroblasts, feed, porcine faeces, body
     fluids and tissues). HPLC was carried out on a C-18 column using
     gradient elution and ion pairing techniques with 1-heptanesulfonic acid
     as ion pairing agent under acidic conditions. The detection limit for
     fluorimetric detection at lambda(ex) = 327 nm and lambda(em) = 577 nm
     was 3 nM sanguinarine. CE analyses were performed in 50 mM phosphate-Na
     buffer pH 2.5, with 150 mM SDS used for pre-concentration by the
     sweeping effect. This experimental configuration allows injecting the
     total capillary length with sanguinarine sample. The detection limit for
     UV detection at 285 nm was 12 nM. Both methods are suitable for
     analysing submicromolar quantities of sanguinarine in biological
     materials. The HPLC method is more sensitive than CE because it uses
     fluorescence detection.,
  DOI = 10.1002/jssc.200301378,
  ISSN = 1615-9314,
  Unique-ID = ISI:000183660100007,
  

• P. Bednar, P. Bartak, K. Lemr, J. Sevcik, and Z. Stransky, “Analysis of 3-quinuclidinol derivatives by capillary electrophoresis,” JOURNAL OF SEPARATION SCIENCE, vol. 26, iss. 8, pp. 709-714, 2003.
  [Bibtex]

  @article ISI:000183660100011,
  Author = Bednar, P and Bartak, P and Lemr, K and Sevcik, J and Stransky, Z,
  Title = Analysis of 3-quinuclidinol derivatives by capillary electrophoresis,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2003,
  Volume = 26,
  Number = 8,
  Pages = 709-714,
  Month = JUN,
  Note = Symposium on Advances in Chromatography and Electrophoresis and Chiranal
     2002, OLOMOUC, CZECH REPUBLIC, JUL 24-27, 2002,
  Abstract = Capillary electrophoresis with indirect UV detection was applied to the
     analysis of a mixture of 3-quinuclidinol and its four quaternary
     derivatives (N-methyl, N-ethyl, N-propyl, and N-isopropyl derivative).
     10 mM imidazole acetate buffer, pH = 4.0 offers suitable detection
     sensitivity (LOD = 1 mumol L-1) and permits separation of the mixture
     except for the pair 3-quinuclidinol- N-methyl derivative. The separation
     of all analytes was achieved on addition of 15 mmol L-1
     beta-cyclodextrin or 25\% (w/w) polyethylene glycol 2000 to the
     background electrolyte. The optimized method was employed for the
     analysis of pond water spiked with these analytes. Actual ionic
     mobilities of the studied compounds were measured using mobility
     standards (potassium, sodium, tetramethyl- and tetrabutylammonium). The
     migration index was derived as another identification parameter based on
     migration data and the precision of the obtained values is discussed in
     brief.,
  DOI = 10.1002/jssc.200301380,
  ISSN = 1615-9314,
  Unique-ID = ISI:000183660100011,
  

• P. Bartak, P. Bednar, L. Cap, L. Ondrakova, and Z. Stransky, “SPME – A valuable tool for investigation of flower scent,” JOURNAL OF SEPARATION SCIENCE, vol. 26, iss. 8, pp. 715-721, 2003.
  [Bibtex]

  @article ISI:000183660100012,
  Author = Bartak, P and Bednar, P and Cap, L and Ondrakova, L and Stransky, Z,
  Title = SPME - A valuable tool for investigation of flower scent,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2003,
  Volume = 26,
  Number = 8,
  Pages = 715-721,
  Month = JUN,
  Note = Symposium on Advances in Chromatography and Electrophoresis and Chiranal
     2002, OLOMOUC, CZECH REPUBLIC, JUL 24-27, 2002,
  Abstract = A novel Headspace Solid Phase Microextraction (HS-SPME) protocol is
     proposed for the analysis of floral scent. Volatile compounds emitted
     from the flower are collected on a Carboxen/PDMS fiber for 1 hour,
     transferred to the GC, and analyzed by GC/ MS. The method completely
     eliminates the use of organic solvents, does not require special
     instrumentation, and may readily be performed in the field without
     access to mains electricity and other energy supplies. The method is
     robust, sensitive, and reduces the sampling stress on the investigated
     plant. Since enzymatic reactions in living flowers may cause changes in
     the composition of emitted fragrance, dried rosemary (Rosmarinus
     officinalis L.) was used as a stable standard for the method development
     and optimization. In addition, grape wine was also suggested as
     homogeneous, bio-compatible, and relatively stable standard of
     pronounced and typical scent for the same purpose. The optimized method
     was used for the comparative investigation of the fragrances emitted by
     two different species - Lathyrus vernus (L.) and Orchis pallens (L.).
     Several monoterpenes (C10 compounds) were found as the main fragrance
     components of lathyrus, while sesquiterpenes (C15 compounds) were
     typical for the orchid.,
  DOI = 10.1002/jssc.200301381,
  ISSN = 1615-9314,
  Unique-ID = ISI:000183660100012,
  

2002

• P. Bednar, K. Lemr, P. Bartak, J. Sevcik, J. Hlavac, J. Styskala, I. Wiedermannova, and Z. Stransky, “Capillary electrophoresis/mass spectrometry: a promising tool for the
  control of some physiologically hazardous compounds. I – Derivatives of
  3-quinuclidinol,” JOURNAL OF MASS SPECTROMETRY, vol. 37, iss. 12, pp. 1213-1218, 2002.
  [Bibtex]

  @article ISI:000180149400005,
  Author = Bednar, P and Lemr, K and Bartak, P and Sevcik, J and Hlavac, J and
     Styskala, J and Wiedermannova, I and Stransky, Z,
  Title = Capillary electrophoresis/mass spectrometry: a promising tool for the
     control of some physiologically hazardous compounds. I - Derivatives of
     3-quinuclidinol,
  Journal = JOURNAL OF MASS SPECTROMETRY,
  Year = 2002,
  Volume = 37,
  Number = 12,
  Pages = 1213-1218,
  Month = DEC,
  Note = 20th Meeting on Mass Spectrometry, FIERA DE PRIMIERO, ITALY, MAY 12-16,
     2002,
  Abstract = A method based on the coupling of capillary electrophoresis with mass
     spectrometry (CE/MS) was developed for the monitoring of 3-quinuclidinol
     and its four N-alkyl derivatives (methyl, ethyl, propyl and isopropyl
     derivatives). A fragmentation study (collision-induced dissociation of
     ions in an ion trap) and optimization of the ion optics set-up for CE/MS
     experiments using direct infusion of a methanolic solution of the
     standards into the mass spectrometer were carried out in advance.
     Molecular ions of all quaternary compounds and the quasi-molecular ion
     [M + H](+) of free 3-quinuclidinol prevail in the mass spectra. In the
     MS/MS of propyl and isopropyl derivatives, the elimination of the alkyl
     chain dominates, leading to the ion at m/z 128. The fragmentation of the
     other compounds is more complex. Previous CE separation of the mixture
     of isobaric propyl and isopropyl derivatives is necessary for their
     unambiguous identification. A 10 mm ammonium acetate buffer (pH 4.0) is
     the optimum running electrolyte, allowing the CE separation of methyl,
     ethyl, propyl and isopropyl derivatives. A 0.5\% (v/v) solution of
     acetic acid in methanol provides sufficient detection sensitivity when
     used as the sheath liquid. Limits of detection of 0.1 ppm for
     3-quinuclidinol and 0.05 ppm for quaternary derivatives were achieved
     under the optimum conditions. The optimized method was applied to the
     determination of 3-quinuclidinol and related quaternary derivatives
     spiked into a sample of pond water. The experimental set-up for CE/MS/MS
     was investigated, which strongly increases the identification capability
     of the technique. Copyright (C) 2002 John Wiley Sons, Ltd.,
  DOI = 10.1002/jms.380,
  ISSN = 1076-5174,
  Unique-ID = ISI:000180149400005,
  

2001

• A. Gavenda, P. Bednar, P. Bartak, P. Adamovsky, J. Sevcik, P. Tzoumas, and J. Ulrichova, “Estimation of partition coefficients by MEKC – Part I: Potential
  pharmaceuticals based on 2-guinolone and 2-indolone,” JOURNAL OF SEPARATION SCIENCE, vol. 24, iss. 9, pp. 723-728, 2001.
  [Bibtex]

  @article ISI:000172109200005,
  Author = Gavenda, A and Bednar, P and Bartak, P and Adamovsky, P and Sevcik, J
     and Tzoumas, P and Ulrichova, J,
  Title = Estimation of partition coefficients by MEKC - Part I: Potential
     pharmaceuticals based on 2-guinolone and 2-indolone,
  Journal = JOURNAL OF SEPARATION SCIENCE,
  Year = 2001,
  Volume = 24,
  Number = 9,
  Pages = 723-728,
  Month = SEP,
  Abstract = The octanol-water partition coefficients of 18 potential pharmaceuticals
     derived from 2-quinolone and 2-indolone were measured by means of MEKC.
     Their values range from 2.2 to 4.7 (expressed in logarithmic form). The
     critical micelle concentration (CIVIC) of SIDS was determined
     conductometrically and the partial specific volumes of the micelles in
     the buffer (25 mM sodium borate, pH 9.5) were calculated from density
     data. The values were used for determination of the phase ratio and for
     estimation of the micelle-water (K-mw) and octanol-water (K-ow)
     partition coefficients. Direct calculation of K-ow from the easily
     measurable capacity factors (le) gives results comparable with those
     laboriously determined from K-mw. Extrapolation of the micellar time
     from the set of migration times of alkylbenzene standards proved to be
     viable alternative to measurements using a marker.,
  DOI = 10.1002/1615-9314(20010901)24:9<723::AID-JSSC723>3.0.CO;2-U,
  ISSN = 1615-9314,
  Unique-ID = ISI:000172109200005,
  

• A. Gavenda, J. Sevcik, J. Psotova, P. Bednar, P. Bartak, P. Adamovsky, and V. Simanek, “Determination of anthracycline antibiotics doxorubicin and daunorubicin
  by capillary electrophoresis with UV absorption detection,” ELECTROPHORESIS, vol. 22, iss. 13, pp. 2782-2785, 2001.
  [Bibtex]

  @article ISI:000170699400024,
  Author = Gavenda, A and Sevcik, J and Psotova, J and Bednar, P and Bartak, P and
     Adamovsky, P and Simanek, V,
  Title = Determination of anthracycline antibiotics doxorubicin and daunorubicin
     by capillary electrophoresis with UV absorption detection,
  Journal = ELECTROPHORESIS,
  Year = 2001,
  Volume = 22,
  Number = 13,
  Pages = 2782-2785,
  Month = AUG,
  Abstract = Sweeping preconcentration and electrokinetic injection was used for the
     capillary electrophoretic analysis of trace amounts of biologically
     active anthracyclines with UV absorption detection. Phosphate buffer
     (100 mM), pH 2.5, with addition of 40\% v/v methanol was used as
     background electrolyte (BGE). Sodium dodecyl sulfate (150 mM) was added
     to BGE in the inlet vial as the sweeping agent. The system enables
     effective separation of anthracyclines as well as cleanup from matrix
     impurities. Sweeping preconcentration of sample provides an excellent
     detection limit (1 x 10(-9) mol L-1). The method was applied for the
     determination of therapeutic levels of doxorubicin in real plasma
     samples.,
  DOI = 10.1002/1522-2683(200108)22:13<2782::AID-ELPS2782>3.0.CO;2-I,
  ISSN = 0173-0835,
  Unique-ID = ISI:000170699400024,
  

• P. Bartak, P. Bednar, D. Friedecky, A. Haviger, and J. Sevcik, “Fast analysis of antibacterial isothiazolones by capillary
  electrophoresis,” JOURNAL OF CHROMATOGRAPHY B, vol. 758, iss. 2, pp. 323-325, 2001.
  [Bibtex]

  @article ISI:000169712400024,
  Author = Bartak, P and Bednar, P and Friedecky, D and Haviger, A and Sevcik, J,
  Title = Fast analysis of antibacterial isothiazolones by capillary
     electrophoresis,
  Journal = JOURNAL OF CHROMATOGRAPHY B,
  Year = 2001,
  Volume = 758,
  Number = 2,
  Pages = 323-325,
  Month = JUL 15,
  Abstract = Some technical aspects influencing the total time of CE analysis are
     discussed. A high throughput electrophoretic system based on micellar
     electrokinetic chromatography (MEKC) is demonstrated as an example. A
     short capillary, strong electric field, alkaline buffer (pH 9.5)
     generating strong electroosmotic flow, and parallel hydrodynamic
     pressure allow the separation of two uncharged isothiazolone derivatives
     within 45 s. (C) 2001 Elsevier Science B.V. All rights reserved.,
  DOI = 10.1016/S0378-4347(01)00198-0,
  ISSN = 0378-4347,
  Unique-ID = ISI:000169712400024,
  

• P. Bednar, Z. Aturki, Z. Stransky, and S. Fanali, “Chiral analysis of UV nonabsorbing compounds by capillary
  electrophoresis using macrocyclic antibiotics: 1. Separation of aspartic
  and glutamic acid enantiomers,” ELECTROPHORESIS, vol. 22, iss. 11, pp. 2129-2135, 2001.
  [Bibtex]

  @article ISI:000170175800002,
  Author = Bednar, P and Aturki, Z and Stransky, Z and Fanali, S,
  Title = Chiral analysis of UV nonabsorbing compounds by capillary
     electrophoresis using macrocyclic antibiotics: 1. Separation of aspartic
     and glutamic acid enantiomers,
  Journal = ELECTROPHORESIS,
  Year = 2001,
  Volume = 22,
  Number = 11,
  Pages = 2129-2135,
  Month = JUL,
  Note = 3rd International Symposium on Capillary Electrophoresis and Related
     Microscale Techniques (APCE 2000), HONG KONG, PEOPLES R CHINA, JUN
     14-17, 2000,
  Abstract = Glycopeptide antibiotics, namely vancomycin or teicoplanin, were
     evaluated in capillary electrophoresis for the analysis of UV
     nonabsorbing compounds such as aspartic and glutamic acid enantiomers.
     Electrophoretic runs were performed in laboratory-made
     polyacrylamide-coated capillaries using the partial filling-counter
     current method in order to avoid the presence on the detector path of
     the absorbing chiral selector. The background electrolyte consisted of
     an aqueous or aqueous-organic buffer in the pH range of 4.5-6.5 of
     sorbic acid/histidine and the appropriate concentration of chiral
     selector. Several experimental parameters such as antibiotic
     concentration and type, buffer pH, organic modifier, type and
     concentration of absorbing co-ion (for the indirect UV detection) were
     studied in order to find the optimum conditions for the chiral
     resolution of the two underivatized amino acids in their enantiomers.
     Among the two investigated chiral selectors, vancomycin resulted to be
     the most useful chiral selector allowing relatively high chiral
     resolution of the studied compounds even at low concentration. The
     optimized method (10 mM sorbic acid/histidine, pH 5, and 10 mM of
     vancomycin) was used for the analysis of real samples such as teeth
     dentine and beer.,
  DOI = 10.1002/1522-2683(20017)22:11<2129::AID-ELPS2129>3.0.CO;2-J,
  ISSN = 0173-0835,
  Unique-ID = ISI:000170175800002,
  

• P. Bednar, P. Bartak, P. Adamovsky, A. Gavenda, J. Sevcik, and Z. Stransky, “Capillary electrophoresis of methylderivatives of quinolines. I,” JOURNAL OF CHROMATOGRAPHY A, vol. 917, iss. 1-2, pp. 319-329, 2001.
  [Bibtex]

  @article ISI:000168613500030,
  Author = Bednar, P and Bartak, P and Adamovsky, P and Gavenda, A and Sevcik, J
     and Stransky, Z,
  Title = Capillary electrophoresis of methylderivatives of quinolines. I,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2001,
  Volume = 917,
  Number = 1-2,
  Pages = 319-329,
  Month = MAY 11,
  Abstract = Migration behavior of quinoline, isoquinoline and related
     methylderivatives has been investigated with respect to the influence of
     running buffer acidity and to the presence of polyethylene glycol (PEG)
     2000 as additive. Dissociation constants and ionic mobilities were
     determined by capillary electrophoresis (CE). Mobility and viscosity
     measurements in PEG containing buffers show that analyte transport is
     not in accordance with Walden's rule and microviscosity plays the role
     in analyte retardation. Variation of pH and PEG concentration provides
     the optimal conditions for the CE separation of methylquinolines (0.0176
     M acetate-Tris buffer, pH 5.5, 10\% PEG 2000). Analysis of industrial
     mixture (isoquinoline fraction from distillation of coal tar) was
     performed and good agreement with gas chromatographic results was found.
     (C) 2001 Elsevier Science B.V. All rights reserved.,
  DOI = 10.1016/S0021-9673(01)00703-8,
  ISSN = 0021-9673,
  Unique-ID = ISI:000168613500030,
  

• J. Spilkova, P. Bednar, and R. Stroblikova, “Capillary electrophoretic analysis of hydroxycinnamic acids from Ononis
  arvensis L.,” PHARMAZIE, vol. 56, iss. 5, pp. 424-425, 2001.
  [Bibtex]

  @article ISI:000168623900017,
  Author = Spilkova, J and Bednar, P and Stroblikova, R,
  Title = Capillary electrophoretic analysis of hydroxycinnamic acids from Ononis
     arvensis L.,
  Journal = PHARMAZIE,
  Year = 2001,
  Volume = 56,
  Number = 5,
  Pages = 424-425,
  Month = MAY,
  ISSN = 0031-7144,
  Unique-ID = ISI:000168623900017,
  

2000

• P. Bartak, D. Pechova, P. Tarkowski, P. Bednar, M. Kotoucek, Z. Stransky, and R. Vespalec, “Determination of the first dissociation constant of 6-benzylaminopurine
  – A comparison of methods,” ANALYTICA CHIMICA ACTA, vol. 421, iss. 2, pp. 221-229, 2000.
  [Bibtex]

  @article ISI:000089383000011,
  Author = Bartak, P and Pechova, D and Tarkowski, P and Bednar, P and Kotoucek, M
     and Stransky, Z and Vespalec, R,
  Title = Determination of the first dissociation constant of 6-benzylaminopurine
     - A comparison of methods,
  Journal = ANALYTICA CHIMICA ACTA,
  Year = 2000,
  Volume = 421,
  Number = 2,
  Pages = 221-229,
  Month = SEP 29,
  Abstract = The dissociation constant of 6-benzylaminopurine was measured by three
     independent methods: capillary zone electrophoresis, ultraviolet
     spectrometry and differential pulse voltammetry. Titration curves,
     obtained from electrophoretic and spectral data, were evaluated by
     standard procedure using Henderson-Haselbalch equation and by nonlinear
     computer fitting. Significantly lower values of standard deviations were
     obtained by using of computer fitting. Dissociation constant pK(a)
     =4.01+/-0.03, 4.10+/-0.07 and 4.24+/-0.12 have been obtained for
     6-benzylaminopurine from electrophoretic, spectral and voltammetric
     data, respectively. (C) 2000 Elsevier Science B.V. All rights reserved.,
  DOI = 10.1016/S0003-2670(00)01037-0,
  ISSN = 0003-2670,
  Unique-ID = ISI:000089383000011,
  

• P. Bartak, P. Bednar, Z. Stransky, P. Bocek, and R. Vespalec, “Determination of dissociation constants of cytokinins by capillary zone
  electrophoresis,” JOURNAL OF CHROMATOGRAPHY A, vol. 878, iss. 2, pp. 249-259, 2000.
  [Bibtex]

  @article ISI:000087244400009,
  Author = Bartak, P and Bednar, P and Stransky, Z and Bocek, P and Vespalec, R,
  Title = Determination of dissociation constants of cytokinins by capillary zone
     electrophoresis,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 2000,
  Volume = 878,
  Number = 2,
  Pages = 249-259,
  Month = MAY 12,
  Note = 11th International Symposium on Capillary Electrophoresis Techniques
     (ITP 98), VENICE, ITALY, OCT 04-07, 1998,
  Abstract = A method for the pK(a) determination, based on mobility data measured by
     capillary zone electrophoresis, was applied to cytokinins and their
     analogs. The combination of charged mobility standards with an uncharged
     electroosmosis marker, injected in the uncoated capillary simultaneously
     with the measured substances, allows one to minimize the number of runs,
     reduce their duration and, in addition, to inform on the run-to-run
     stability of electroosmosis and on contingent side-effects. pK(a) values
     of investigated cytokinins and their analogs ranged from 2.8 to 4.0 at
     25 degrees C in the phosphate and acetate buffers of ionic strength
     0.015 M. Standard deviations of the constants, obtained by the
     non-linear fitting of equations for the pK(a) calculation, were
     3-5-times lower than standard deviations from the linear fitting or from
     the point-to-point calculation utilizing the Hendersson-Haselbalch
     equation. The equation of Boltzman sigmoid offers two checks on
     reliability of effective mobilities that serve as the raw data in the
     pK(a) calculation. (C) 2000 Elsevier Science B.V. All rights reserved.,
  DOI = 10.1016/S0021-9673(00)00245-4,
  ISSN = 0021-9673,
  Unique-ID = ISI:000087244400009,
  

• P. Bartak, P. Bednar, L. Kubacek, and Z. Stransky, “Advanced statistical evaluation of complex formation constant from
  electrophoretic data,” ANALYTICA CHIMICA ACTA, vol. 407, iss. 1-2, pp. 327-336, 2000.
  [Bibtex]

  @article ISI:000085377900036,
  Author = Bartak, P and Bednar, P and Kubacek, L and Stransky, Z,
  Title = Advanced statistical evaluation of complex formation constant from
     electrophoretic data,
  Journal = ANALYTICA CHIMICA ACTA,
  Year = 2000,
  Volume = 407,
  Number = 1-2,
  Pages = 327-336,
  Month = FEB 29,
  Abstract = A new method for the estimation of complex formation constants is
     presented. The method is based on electrophoretically measured effective
     mobilities and applied to the estimation of the complex formation
     constant of interaction between nitrogen heterocyclic bases and
     cyclodextrines. The calculation of constants is based on the
     linearization of the dependence between effective mobility and the
     cyclodextrine concentration and the application of an advanced
     statistical evaluation procedure. Complex formation constants 14.8 and
     63.21 mol(-1) were obtained for the interaction of pyridinium and
     benzylaminopurinium with dimethyl-beta-cyclodextrine (DM-beta-CD),
     respectively. Constants in the order of magnitude 10(1)-10(2) l mol(-1)
     were obtained for some other purine derivatives.
     The proposed procedure, in connection with the mathematical software for
     matrix operations, is rather simple and gives much more valuable outputs
     than commonly used concepts. (C) 2000 Elsevier Science B.V. All rights
     reserved.,
  DOI = 10.1016/S0003-2670(99)00785-0,
  ISSN = 0003-2670,
  Unique-ID = ISI:000085377900036,
  

1999

• P. Bednar, Z. Stransky, P. Bartak, and P. Adamovsky, “Polyethylene glycol as a separation medium for capillary zone
  electrophoretic analysis of pyridine derivatives,” JOURNAL OF CHROMATOGRAPHY A, vol. 838, iss. 1-2, pp. 89-99, 1999.
  [Bibtex]

  @article ISI:000079889700010,
  Author = Bednar, P and Stransky, Z and Bartak, P and Adamovsky, P,
  Title = Polyethylene glycol as a separation medium for capillary zone
     electrophoretic analysis of pyridine derivatives,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 1999,
  Volume = 838,
  Number = 1-2,
  Pages = 89-99,
  Month = APR 9,
  Note = 11th International Symposium on Capillary Electroseparation Techniques,
     VENICE, ITALY, OCT 04-07, 1998,
  Abstract = The influence of the addition of polyethylene glycol (PEG) to the
     background electrolyte on the capillary zone electrophoretic (CZE)
     separation of alkylpyridines was studied. It was found out that
     interactions between polyethylene glycol chains and protonized pyridine
     bases cause the increasing of the resolution. The separation of a model
     mixture of pyridine, all isomers of methylpyridine, and some other alkyl
     and aryl derivatives was performed. The separation was complete except
     for some dimethylpyridines. The influence of the co-ion was discussed,
     too. Polyethylene glycol solution as a separation medium was used for
     the capillary zone electrophoretic determination of pyridine bases in an
     industrial mixture. (C) 1999 Elsevier Science B.V. All rights reserved.,
  DOI = 10.1016/S0021-9673(98)00973-X,
  ISSN = 0021-9673,
  Unique-ID = ISI:000079889700010,
  

• P. Bednar, Z. Stransky, J. Sevcik, and V. Dostal, “Precise measurement of mobility from the length of isotachophoretic
  zones,” JOURNAL OF CHROMATOGRAPHY A, vol. 831, iss. 2, pp. 277-284, 1999.
  [Bibtex]

  @article ISI:000078461000012,
  Author = Bednar, P and Stransky, Z and Sevcik, J and Dostal, V,
  Title = Precise measurement of mobility from the length of isotachophoretic
     zones,
  Journal = JOURNAL OF CHROMATOGRAPHY A,
  Year = 1999,
  Volume = 831,
  Number = 2,
  Pages = 277-284,
  Month = JAN 29,
  Abstract = A new method for the precise estimation of the ionic mobilities was
     described. The procedure issues from the measurement of the time of
     moving boundary between leading and terminating electrolyte in the
     geometrically and thermometrically well-defined device for capillary
     isotachophoresis. An indirect method for the determination of mobilities
     of low movable ionic species was also derived. The ionic mobilities of
     some anionic pesticides and cationic drugs were measured. The procedure
     can be used for estimation of transference numbers and also their
     temperature coefficient. (C) 1999 Elsevier Science B.V. All rights
     reserved.,
  DOI = 10.1016/S0021-9673(98)00919-4,
  ISSN = 0021-9673,
  Unique-ID = ISI:000078461000012,