Nanotechnology in analytical chemistry

Head of the Division:

Prof. Karel Lemr, Ph.D.


About the group:

The group focuses on describing and modelling analytical processes during the transition to the nanoscale (the ionization of substances, nanoseparation beds, the influence of nanoparticles on the interaction of radiation and the analyte, electrochemical conversions) and characterizing nanomaterials using the methods of analytical chemistry (e.g. capillary electrophoresis). The group also works on the development of new analytical tools (ion sources, separation systems, sensitive detection systems) and advanced analytical methods (lower detection thresholds, accelerated analyses). Other favoured directions include the development of methods for observing the conversion of toxic and pharmacologically interesting substances, and the description of the interactions of these substances with living organisms. The group is also concerned with basic research on ionization and fragmentation in mass spectrometry, preconcentration, and chromatographic and electrophoretic separation. The knowledge obtained serves as a basis for e.g. analyzing foods or in forensic analysis.


Results highlights:

V. Melenovsky, J. Petrak, T. Mracek, J. Benes, B.A. Borlaug, H. Nuskova, T. Pluhacek, J. Spatenka, J. Kovalcikova, Z. Drahota, J. Kautzner, J. Pirk, J. Houstek: Myocardial iron content and mitochondrial function in human heart failure: a direct tissue analysis. European Journal of Heart Failure, vol. 19, iss. 4, pp. 522-530, 2017.
DOI: 10.1002/ejhf.640

T. Mašková, L. Hárendarčíková, J. Petr: Determination of Escherichia coli in urine using a low-cost foil-based microfluidic device. Talanta, vol. 170, pp. 36–40, 2017.
DOI: 10.1016/j.talanta.2017.03.089

D. Baron, C. Cacho, J. Petr: Electrokinetic preconcentration of magnetite core – carboxylic shell nanoparticles by capillary electrophoresis. Journal of Chromatography A, vol. 1499, pp. 217–221, 2017.
DOI: 10.1016/j.chroma.2017.03.079

L. Skultety, P. Frycak, Ch. Qiu, J. Smuts, L. Shear-Laude, K. Lemr, J.X. Mao, P. Kroll, K.A. Schug, A. Szewczak, C. Vaught, I. Lurie, V. Havlicek: Resolution of isomeric new designer stimulants using gas chromatography – Vacuum ultraviolet spectroscopy and theoretical Computations. Analytica Chimica Acta, vol. 971, pp. 55-67, 2017.
DOI: 10.1016/j.aca.2017.03.023

P. Janoš, J. Henych, J. Pfeifer, N. Zemanová, V. Pilařová, D. Milde, T. Opletal, J. Tolasz, M. Malýd, V. Štengl: Nanocrystalline cerium oxide prepared from a carbonate precursor and its ability to breakdown biologically relevant organophosphates. Environmental Science-Nano, vol. 4, iss. 6, pp. 1283–1293, 2017.
DOI: 10.1039/c7en00119c

L. Borovcova, M. Hermanova, V. Pauk, M. Šimek, V. Havlíček, K. Lemr: Simple area determination of strongly overlapping ion mobility peaks. Analytica Chimica Acta, vol. 981, pp. 71-79, 2017.
DOI: 10.1016/j.aca.2017.05.003

V. Pauk, T. Pluháček, V. Havlíček, K.Lemr: Ultra-high performance supercritical fluid chromatography-mass spektrometry procedure for analysis of mono saccharides from plant gum binders. Analytica Chimica Acta, vol. 989, pp. 112-120, 2017.
DOI: 10.1016/j.aca.2017.07.036

L. Hárendarčíková, D. Baron, A. Šebestová, J. Rozsypal, J. Petr: True lab-in-a-syringe technology for bioassays. TALANTA, vol. 174, pp. 285-288, 2017.
DOI: 110.1016/j.talanta.2017.06.017

L. Kučera, O. Kurka, P. Barták, P. Bednář: Liquid chromatography/high resolution tandem mass spectrometry – Tool for the study of polyphenol profile changes during micro-scale biogas digestion of grape marcs. CHEMOSPHERE, vol. 166, pp. 463-472, 2017.
DOI: 10.1016/j.chemosphere.2016.09.124

D. Riman, K. Spyrou, A.E. Karantzalis, J. Hrbac, M.I. Prodromidis: Glucose sensing on graphite screen-printed electrode modified by sparking of copper nickel alloys. TALANTA, vol. 165, pp. 466-473, 2017.
DOI: 10.1016/j.talanta.2016.12.064

J. Přichystal, K.A. Schug, K. Lemr, J. Novák, V. Havlíček: Structural Analysis of Natural Products. Anal. Chem., 88 (21), pp 10338–10346, 2016.
DOI: 10.1021/acs.analchem.6b02386

M. Švidrnoch, A. Přibylka, V. Bekárek, J. Ševčík, V. Smolka, V. Maier: Enantioseparation of d,l-2-hydroxyglutaric acid by capillaryelectrophoresis with tandem mass spektrometry-Fast and efficienttool for d- and l-2- hydroxyglutaracidurias diagnosis. Journal of Chromatography A, vol. 1467, pp. 383–390, 2016.
DOI: 10.1016/j.chroma.2016.05.095

M. Jarošová, D. Walaszek, B. Wagner, D. Milde and E. Bulska: Influence of temperature on laser ablation fractionation during ICP-MS analysis for 213 nm and 266 nm laser wavelength micro-sampling. J. Anal. At. Spectrom., vol. 31, pp. 2089–2093, 2016.
DOI: 10.1039/c6ja00182c

P. Kučerová, J. Skopalová*, L. Kučera, J. Táborský, H. Švecová, K. Lemr, P. Cankař, P. Barták: Electrochemical oxidation of 5-hydroxymethyl tolterodine and identification of its oxidation products using liquid chromatography andmass spektrometry. Electrochimica Acta, vol. 215, pp. 617–625, 2016.
DOI: 10.1016/j.electacta.2016.08.137

J. Znaleziona, D. Drahoňovský, B. Drahoš, J. Ševčík, V. Maier: Novel cationic coating agent for protein separation by capillary electrophoresis. J. Sep. Sci., vol. 39, iss. 12, pp. 2406–2412, 2016.
DOI: 10.1002/jssc.201501349

P. Ginterová, J. Znaleziona*, R. Knob, M. Douša, J. Petr, J. Ševčík: Enantiomeric purity control of R-cinacalcet in pharmaceutical product by capillary electrophoresis. Chemical Papers, vol. 70, iss. 8, pp. 1024–1030, 2016.
DOI: 10.1515/chempap-2016-0047

B. Papouskova, M. Bernard, J. Ottenschlager, J. Karban, P. Velisek, J. Hrbac, J. Sykora, J. Storch, J. Vacek: A comprehensive LC/MS analysis of novel cyclopentenedione library. Journal of Pharmaceutical and Biomedical Analysis, vol. 128, pp. 342–351, 2016.
DOI: 10.1016/j.jpba.2016.05.048

O. Kurka, L. Kučera, P. Bednář: Analytical and semipreparative chiral separation of cis-itraconazole on cellulose stationary phases by high-performance liquid chromatography. J. Sep. Sci., vol. 39, iss. 14, pp. 2736–2745, 2016.

P. Fryčák*, L. Hartmanová, I. Lorencová, Karel Lemr: Screening of synthetic phosphodiesterase-5 inhibitors in herbal dietary supplements using transmission-mode desorption electrospray and high-resolution mass spektrometry. J. Mass Spectrom., vol. 51, iss. 5, pp. 358–362, 2016.
DOI: 10.1002/jms.3754

J. Znaleziona*, V. Maier: The use of PAMAM dendrimers as a dynamic coating for cyclodextrin mediated enantioseparation of selected basic drugs. Anal. Methods, vol. 8, iss.9, pp. 1968–1972, 2016.
DOI: 10.1039/c5ay03070f

L. Hartmanová, I. Lorencová, M. Volný, P. Fryčák, V. Havlíček, H. Chmelíčková, T. Ingr, K. Lemr: Lateral resolution of desorption nanoelectrospray: a nanospray tip without nebulizing gas as a source of primary charged droplets, Analyst vol. 141, Iss. 7, pp. 2150-2154, 2016.
DOI: 10.1039/c5an02665b

T. Pluháček, K. Lemr, D. Ghosh, D. Milde, J. Novák, V. Havlíček: Characterization of microbial siderophores by mass spectrometry, MASS SPECTROMETRY REVIEWS vol. 35, Iss. 1, SI, pp. 35-47, 2016.
DOI: 10.1002/mas.21461

V. Pauk, V. Žihlová, L. Borovcová, V. Havlíček, K. Schug, K. Lemr: Fast separation of selected cathinones and phenylethylamines by supercritical fluid chromatography, Journal of Chromatography A vol. 1423, pp. 169-176, 2015.
DOI: 10.1016/j.chroma.2015.10.061

J. Znaleziona, P. Ginterova, J. Petr,P. Ondra, I. Valka, J. Sevcik, J. Chrastina, V. Maier: Determination and identification of synthetic cannabinoids and their metabolites in different matrices by modern analytical techniques, ANALYTICA CHIMICA ACTA vol. 874, pp. 11-25, 2015.
DOI: 10.1016/j.aca.2014.12.055

P. Kucerova, J. Skopalova, L. Kucera, J. Hrbac, K. Lemr: Electrochemical oxidation of fesoterodine and identification of its oxidation products using liquid chromatography and mass spektrometry, ELECTROCHIMICA ACTA vol. 159, pp. 131-139, 2015.
DOI: 10.1016/j.electacta.2015.01.190

L. Veverková, Š. Hradilová, D. Milde, A. Panáček, J. Skopalová, L. Kvítek, K. Petrželová, R. Zbořil: Accurate determination of silver nanoparticles in animal tissues by inductively coupled plasma mass spectrometry, SPECTROCHIMICA ACTA PART B: ATOMIC SPECTROSCOPY vol. 102, pp. 7-11, 2014.
DOI: 10.1016/j.sab.2014.10.002

C. Cacho, Z. Markova, J. Sevcik, R. Zboril, J. Petr: Study of behavior of carboxylic magnetite core shell nanoparticles on a pH boundary. Journal of Chromatography A vol. 1364, pp. 59-63, 2014.
DOI: 10.1016/j.chroma.2014.08.090

B. Papouskova, H. Fan, K. Lemr, K. A. Schug: Aspects of trapping efficiency and matrix effects in the development of a restricted-access-media-based trap-and-elute liquid chromatography with mass spectrometry method, JOURNAL OF SEPARATION SCIENCE vol. 37, iss. 16, pp. 2192-2199, 2014.
DOI: 10.1002/jssc.201400369

P. Ginterová, B. Sokolová, P. Ondra, J. Znaleziona, J. Petr, J. Ševčík, V. Maier: Determination of mushroom toxins ibotenic acid, muscimol and muscarine by capillary electrophoresis coupled with electrospray tandem mass spectrometry, TALANTA vol. 125, pp. 242-247, 2014.
DOI: 10.1016/j.talanta.2014.03.019

M. Volny, J. Rolfs, B. Hakimi, P. Frycak, T. Schneider, D. Liu, G. Yen, D.T. Chiu, F. Turecek: Nanoliter Segmented-Flow Sampling Mass Spectrometry with Online Compartmentalization, Anal. Chem. vol. 86 (7), pp. 3647–3652, 2014.
DOI: 10.1021/ac500365r

R. Knob, J. Petr, J. Ševčík, V. Maier: Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection, JOURNAL OF SEPARATION SCIENCE, 2013.
DOI: 10.1002/jssc.201300507

V. Pauk, V. Havlíček, B. Papoušková, P. Sulovský, K. Lemr: Simultaneous identification of historical pigments Prussian blue and indigo in paintings by electrospray mass spectrometry, J. MASS SPECTROM. vol. 48, iss. 8, pp. 927-930, 2013.
DOI: 10.1002/jms.3228

L. Li, S. H. Yang, K. Lemr, V. Havlicek, K. A. Schug: Continuous flow-extractive desorption electrospray ionization: Analysis from “non-electrospray ionization-friendly” solvents and related mechanism, ANALYTICA CHIMICA ACTA vol. 769, pp. 84-90, 2013.
DOI: 10.1016/j.aca.2013.01.018

A. Pribylka, A.V. Almeida, M.O. Altmeyer, J. Petr, J. Sevcik, A. Manza, P. Neuzil: Fast spore breaking by superheating, Lab Chip vol. 13, pp. 1695-1698, 2013.
DOI: 10.1039/C3LC41305E

J. Součková, J. Skopalová, H. Švecová, P. Barták: Fused-Silica Capillary Dropping Mercury Electrode for Electrocapillary Measurements, ELECTROANALYSIS vol. 25, iss. 1, pp. 174-178, 2013.
DOI: 10.1002/elan.201200382

V. Havlicek, K. Lemr, K. A. Schug: Current Trends in Microbial Diagnostics Based on Mass Spectrometry, ANAL. CHEM. vol. 85, iss. 2, pp. 790-797, 2013.
DOI: 10.1021/ac3031866

R. Knob, V. Maier, J. Petr, V. Ranc, J. Ševčík: On-line preconcentration of perfluorooctanoic acid and perfluorooctanesulfonic acid by nonaqueous capillary electrophoresis, ELECTROPHORESIS vol. 33, iss. 14, pp. 2159-2166, 2012.
DOI: 10.1002/elps.201100665

P. Fryčák, J. Jirkovský, V. Ranc, P. Bednář, V. Havlíček, K. Lemr: Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid, J. MASS. SPECTROM. vol. 47, iss. 6, pp. 720-726, 2012.
DOI: 10.1002/jms.3006

V. Maier, V. Ranc, M. Švidrnoch, J. Petr, J. Ševčík, E. Tesařová, D. W. Armstrong: Study on the use of boromycin as a chiral selector in capillary electrophoresis, JOURNAL OF CHROMATOGRAPHY A vol. 1237, pp. 128-132, 2012.
DOI: 10.1016/j.chroma.2012.02.073

K. Hron, M. Jelínková, P. Filzmoser, R. Kreuziger, P. Bednář, P. Barták: Statistical analysis of wines using a robust compositional biplot, TALANTA vol. 90, pp. 46-50, 2012.
DOI: 10.1016/j.talanta.2011.12.060

J. Petr, V. Maier: Analysis of microorganisms by capillary electrophoresis, TRAC TRENDS IN ANALYTICAL CHEMISTRY vol. 31, pp. 9-22, 2012.
DOI: 10.1016/j.trac.2011.07.013

K. A. Schug, C. Serrano, P. Fryčák: Controlled band dispersion for quantitative binding determination and analysis with electrospray ionization-mass spectrometry, MASS SPECTROM. REV. vol. 29, iss. 5, pp. 806-829, 2010.
DOI: 10.1002/mas.20267

L. Hartmanova, V. Ranc, B. Papouskova, P. Bednar, V. Havlicek, K. Lemr: Fast profiling of anthocyanins in wine by desorption nano-electrospray ionization mass spectrometry, JOURNAL OF CHROMATOGRAPHY A vol. 1217, iss. 25, pp. 4223-4228, 2010.
DOI: 10.1016/j.chroma.2010.03.018


Certificate of GMP Compliance of a Manufacturer


Setting up the geometrical parameters of desorption nanoelectrospray


Inductively Coupled Plasma Mass Spectrometer for trace metal analysis

Two young researchers carrying out experiments in a lab

Gas chromatography-mass spectrometry for identification and quantitation of volatile compounds

2D ultraperformance liquid chromatography for complex mixture separation

2D ultraperformance liquid chromatography for complex mixture separation.

Ion mobility-mass spectrometry for challenging analysis of organic compounds

Ion mobility-mass spectrometry for challenging analysis of organic compounds.


Separation of abused “designer” cathinones using CE-ESI-MS/MS.


Analysis of volatile compounds isolated from food sample.


Imaging of Aspergillus fumigatus infection on GMS-stained lung section (pixel size 5×5 μm).


Fast screening of new designer drugs by supercritical fluid chromatography/mass spectrometry.