Mgr., Bc. et Bc. Markéta Havrdová, Ph.D.

Research activities:

Microscopy imaging of biological samples (light/fluorescent microscopy, electron microscopy, time – lapse microscopy), Cytotoxicity analysis of nanomaterials (MTT assays, measuring of ROS), Intracellular detection of nanomaterials (such as SPIO nanoparticles, carbon quantum dots)

Employment:

  • 2012 – now, Junior Researcher, Regional Centre of Advanced Technologies and Materials, Palacký University in Olomouc

Education:

  • Bachelor’s degree: 2010, Biophysics, University of South Bohemia in České Budějovice
  • Master‘s degree: 2012, Nanotechnology, Palacký University in Olomouc
  • 2nd Bacelor‘s degree: 2014, Optometry, Palacký University in Olomouc
  • Doctoral degree: 2017, Applied Physics, Palacký University in Olomouc

Internship

  • Institute Jožef Stefan, Laboratory of Biophysics Condensed matter department, Slovenia.

International Conferences:

  • Nanosafe 2014, 17. – 21. 11. 2014, Grenoble (France); Oral presentation: Cytotoxicity Evaluations of Carbon Dots with Different Surface Charge
  • 6th International Conference Nanocon 2014, 5. – 7. 11. 2014, Brno (Czech Republic), poster: Cytotoxicity Evaluations of Carbon Dots with Different Surface Charge
  • 58th meeting of the Biopthysical Society, 15.-19.2. 2014, San Francisco, CA (USA), poster: Antibody – conjugated Superparamagnetic Iron Oxide Nanoparticles for Active targeting of Adenosine Receptors
  • 57th Annual Meeting of the Biophysical Society, 2. – 6. 2. 2013, Philadelphia, PA (USA), poster: Real-Time Imaging of SPION Modified Stem Cells; 3th place in competition The Art of Science Image
  • 4thInternational Conference Nanocon 2012, 23. – 25. 10. 2012, Brno (Czech Republic), poster: Live in vitro monitoring of stem cell labeling process by SPIO nanoparticles using advanced microscopic techniques
  • 3rd International Conference Nanocon 2011, 21. – 23. 9. 2011, Brno (Czech Republic), poster: Morphologies and Proliferation of Rat Mesenchymal Stem Cells (rMSCs) After Internalization of SPIO Nanoparticles Investigated by Various Microscopic Techniques; Prize for the Best Poster

Publications

Malina T., Polakova K., Skopalik J., Milotova V., Hola K., Havrdova M. et al. Carbon dots for in vivo fluorescence imaging of adipose tissue-derived mesenchymal stromal cells. Carbon 152, 434-443, 2019

Vlcek J., Lapcik L., Havrdova M. et al. Flow induced HeLa cell detachment kinetics show that oxygen-containing functional groups in graphene oxide are potent cell adhesion enhancers. Nanoscale 11, 3222-3228, 2019

Cmiel V., Skopalik J., Polakova K., Solar J., Havrdova M., Milde D., Justan I., Magro M., Starcuk Z., Provaznik I, Rhodamine Bound Maghemite As a Long-Term Dual Imaging Nanoprobe of Adipose Tissue-Derived Mesenchymal Stromal Cells.  European Biophysics Journal, doi:10.1007/s00249-016-1187-1

Tucek J., Sofer Z., Bousa D., Pumera M., Hola K., Mala A., Polakova K., Havrdova M., Cepe K., Tomanec O., Zboril R. Air-Stable Superparamagnetic Metal Nanoparticles Entrapped in Graphene Oxide Matrix. Nature Communications 7, doi:10.1038/ncomms12879, 2016

Panacek A., Smekalova M., Vecerova R., Bogdanova K., Roderova M., Kolar M., Kilianova M., Hradilova S., Froning J. P., Havrdova M., Prucek R., Zboril R., Kvitek L. Silver Nanoparticles Strongly Enhance and Restore Bactericidal Activity of Inactive Antibiotics Against Multiresistant Enterobacteriaceae. Colloids and Surfaces B-Biointerfaces 142: 392-399, 2016

Havrdova M., Hola K., Skopalik  J., Tomankova K., Petr M., Cepe K., Polakova K., Tucek J., Bourlinos A. B., Zboril R. Toxicity of Carbon Dots – Effect of Surface Functionalization on The Cell Viability, Reactive Oxygen Species Generation and Cell Cycle. Carbon 99: 238-248, 2016

Panacek  A., Smekalova M., Kilianova M., Prucek R., Bogdanova K., Vecerova R., Kolar M., Havrdova M. et al.: Strong and Nonspecific Synergistic Antibacterial Efficiency of Antibiotics Combined with Silver Nanoparticles at Very Low Concentrations Showing No Cytotoxic Effect. Molecules 21: 2016, doi:10.3390/molecules21010026

Tomankova K., Polakova K., Pizova K., Binder S., Havrdova M., Kolarova M., Kriegova E., Zapletalova J., Malina L., Horakova J., Malohlava J., Kolokithas-Ntoukas A., Kolarova H., Zboril R. In Vitro Cytotoxicity Analysis of Doxorubicin-Loaded/Superparamagnetic Iron Oxide Colloidal Nanoassemblies on MCF7 and NIH3T3 Cell Lines. Int. J. of Nanomedicine 10: 949-961, 2015

Datta K. K. R., Kozak O., Ranc V., Havrdova M., Bourlinos A. B., Safarova K., Hola K., Tomankova K., Zoppellaro G., Otyepka M., Zboril R. Quaternized Carbon Dots Modified Graphene Oxide for Selective Cell Labelling – Controlled Nucleus and Cytoplasm Imaging. Chem. Commun. 50: 10782-10785, 2014

Havrdova M., Polakova K., Skopalik J., Vujtek M., Mokdad A., Homolkova M., Tucek J., Nebesarova J., Zboril R. Field Emission Scanning Electron Microscopy (FE-SEM) as an Approach of Nanoparticles Detection Inside Cells. Micron 67: 149-154, 2014

Hola K., Bourlinos A. B., Kozak O., Berka K., Siskova M. K., Havrdova M., Tucek J., Safarova K., Otyepka M., Giannelis P. E., Zboril R. Photoluminescence Effects of Graphitic Core Size and Surface Functional Groups in Carbon Dots: COO− Induced Red-Shift Emission. Carbon 70: 279-286, 2014

Skopalik J., Polakova K., Havrdova M., Justan I., Magro M., Milde D., Knopfova L., Smarda J., Polakova H., Gabrielova E., Vianello F., Michalek J., Zboril R. Mesenchymal Stromal Cell Labeling by New Uncoated Superparamagnetic Maghemite Nanoparticles in Comparison with Commercial Resovist – an Initial In Vitro Study. Int. J. of Nanomedicine 9: 5355-5372, 2014

Kolářová M., Poláková K., Tománková K., Havrdová M., Marková Z., Zbořil R.: Rapid Cellular Uptake of Superparamagnetic Iron Oxide Nanoparticles by Using Low-Intensity Ultrasound. NANOCON 2013, Conference Proceedings, 578-583, 2014

Skopalík J., Poláková K., Čmiel V., Havrdová M.; Nanočástice v současné biologii a medicíně; Zpravodaj Československé biologické společnosti, Ročník 23, číslo 2, 2013, ISSN 1805 – 9619

Svatakova (Havrdova) M., Polakova K., Skopalik J., Brezina V., Nebesarova J., Zboril R., Live In Vitro Monitoring of Stem Cell Labeling Process by SPIO Nanoparticles Using Advanced Microscopic Techniques, NANOCON 2012, Conference Proceedings, 558-563, 2012