Vítězslav Maier

Email: vitezslav.maier@upol.cz

Location: 17. listopadu 12, Olomouc

Phone: (+420) 585634417

Fax: (+420) 585 634 433

Oblast výzkumu:

Elektromigrační metody, bezkontaktní voddivostní detekce, spojení CE-MS, spojení CE-LIF, separace opticky aktivních látek, on-line prekoncentrace.

Kvalifikace:

Mgr.: analytická chemie, PřF UP Olomouc

Ph.D.: analytická chemie, PřF UP Olomouc

Show publications

Publications

2011

  • [DOI] J. Znaleziona, V. Maier, J. Petr, J. Chrastina, and J. Sevcik, “MEKC Determination of Nilutamide in Human Serum Using Sweeping in High
    Salt Sample Matrix,” CHROMATOGRAPHIA, vol. 74, iss. 1-2, pp. 151-155, 2011.
    [Bibtex]
    @article ISI:000291745400019,
    Author = Znaleziona, Joanna and Maier, Vitezslav and Petr, Jan and Chrastina, Jan
       and Sevcik, Juraj,
    Title = MEKC Determination of Nilutamide in Human Serum Using Sweeping in High
       Salt Sample Matrix,
    Journal = CHROMATOGRAPHIA,
    Year = 2011,
    Volume = 74,
    Number = 1-2,
    Pages = 151-155,
    Month = JUL,
    Abstract = The determination of the antiandrogen drug nilutamide in human serum by
       MEKC using flutamide as an internal standard is described. Several
       parameters influencing the separation, such as the running electrolyte
       composition concerning the micelle concentration and pH, are discussed.
       MEKC separation was achieved within 7 min using 50 mM sodium borate pH
       9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The
       proposed method was applied to determination of nilutamide in spiked
       human serum samples after protein precipitation with acetonitrile. The
       increasing of sensitivity for determination of nilutamide in serum was
       done by sweeping in a high salt concentration sample matrix when the
       injection of a larger volume of sample diluted in 150 mM NaCl was
       applied. The limit of detection after the preconcentration step for
       nilutamide was 26 mu g L(-1).,
    DOI = 10.1007/s10337-011-2019-1,
    ISSN = 0009-5893,
    Unique-ID = ISI:000291745400019,
    
  • [DOI] J. Znaleziona, V. Maier, V. Ranc, and J. Sevcik, “Determination of rosiglitazone and metformin in human serum by CE-ESI-MS,” JOURNAL OF SEPARATION SCIENCE, vol. 34, iss. 10, pp. 1167-1173, 2011.
    [Bibtex]
    @article ISI:000290635400010,
    Author = Znaleziona, Joanna and Maier, Vitezslav and Ranc, Vaclav and Sevcik,
       Juraj,
    Title = Determination of rosiglitazone and metformin in human serum by CE-ESI-MS,
    Journal = JOURNAL OF SEPARATION SCIENCE,
    Year = 2011,
    Volume = 34,
    Number = 10,
    Pages = 1167-1173,
    Month = MAY,
    Abstract = A new method for the determination of anti-diabetic drugs metformin and
       rosiglitazone based on the use of capillary electrophoresis with
       electrospray mass spectrometry was developed. The proposed method
       allowed their separation within 11 min by using 50 mM formic acid at +20
       kV. Positive electrospray ionization and selected ion monitoring
       [M+H](+) of metformin (m/z=130) and rosiglitazone (m/z=358) were
       performed. Several important experimental parameters influencing
       electrospray ionization of metformin and rosiglitazone were studied. The
       final composition of sheath liquid was water/methanol/formic acid
       (50:49.5:0.5, v/v/v), at a flow rate of 2 mu L/min. The developed method
       was applied for the determination of metformin and rosiglitazone
       simultaneously in human serum after protein precipitation with
       acetonitrile. The limits of detection of developed method were 4.42 and
       2.14 ng/mL for rosiglitazone and for metformin, respectively, which is
       sufficient for therapeutic serum concentration levels monitoring for
       both studied drugs.,
    DOI = 10.1002/jssc.201000823,
    ISSN = 1615-9306,
    Unique-ID = ISI:000290635400010,
    
  • [DOI] V. Ranc, A. Stanova, J. Marak, V. Maier, J. Sevcik, and D. Kaniansky, “Preparative isotachophoresis with surface enhanced Raman scattering as a
    promising tool for clinical samples analysis,” JOURNAL OF CHROMATOGRAPHY A, vol. 1218, iss. 2, pp. 205-210, 2011.
    [Bibtex]
    @article ISI:000286700600003,
    Author = Ranc, Vaclav and Stanova, Andrea and Marak, Jozef and Maier, Vitezslav
       and Sevcik, Juraj and Kaniansky, Dusan,
    Title = Preparative isotachophoresis with surface enhanced Raman scattering as a
       promising tool for clinical samples analysis,
    Journal = JOURNAL OF CHROMATOGRAPHY A,
    Year = 2011,
    Volume = 1218,
    Number = 2,
    Pages = 205-210,
    Month = JAN 14,
    Abstract = A surface enhanced Raman scattering (SERS) spectrometry is an
       interesting alternative for a rapid molecular recognition of analytes at
       very low concentration levels. The hyphenation of this technique with
       advanced separation methods enhances its potential as a detection
       technique. Until now, it has been hyphenated mainly with common
       chromatographic and electrophoretic techniques. This work demonstrates
       for a first time a power of preparative isotachophoresis-surface
       enhanced Raman scattering spectrometry (pITP-SERS) combination on the
       analysis of model analyte (buserelin) in a complex biological sample
       (urine). An off-line identification of target analyte was performed
       using a comparison of Raman spectra of buserelin standard with spectra
       obtained by the analyses of the fractions from preparative
       isotachophoretic runs. SERS determination of buserelin was based on the
       method of standard addition to minimize the matrix effects. The
       linearity of developed method was obtained in the concentration range
       from 0.2 to 1.5 nmol L(-1) with coefficient of determination 0.991. The
       calculated limit of detection is in tens of pico mols per liter. (C)
       2010 Elsevier B.V. All rights reserved.,
    DOI = 10.1016/j.chroma.2010.11.025,
    ISSN = 0021-9673,
    Unique-ID = ISI:000286700600003,
    

2010

  • [DOI] D. Jirovsky, Z. Bartosova, J. Skopalova, and V. Maier, “Electrochemical characterization of repaglinide and its determination in
    human plasma using liquid chromatography with dual-channel coulometric
    detection,” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL
    AND LIFE SCIENCES
    , vol. 878, iss. 31, pp. 3243-3248, 2010.
    [Bibtex]
    @article ISI:000285118100006,
    Author = Jirovsky, David and Bartosova, Zdenka and Skopalova, Jana and Maier,
       Vitezslav,
    Title = Electrochemical characterization of repaglinide and its determination in
       human plasma using liquid chromatography with dual-channel coulometric
       detection,
    Journal = JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL
       AND LIFE SCIENCES,
    Year = 2010,
    Volume = 878,
    Number = 31,
    Pages = 3243-3248,
    Month = DEC 1,
    Abstract = A simple fast and sensitive HPLC method employing dual-channel
       coulometric detection for the determination of repaglinide in human
       plasma is presented The assay involved extraction of repaglinide by
       ethyl acetate and isocratic reversed-phase liquid chromatography with
       dual-channel coulometric detection The mobile phase composition was 50mM
       disodium hydrogen phosphate/acetonitrile (60 40 v/v) pH of the mobile
       phase 7 5 set up with phosphoric acid For all analyses the first cell
       working potential was +380 mV the second was +750 mV (vs Pd/H(2))
       Calibration curve was linear over the concentration range of 5-500 nmol
       L(-1) Rosiglitazone was used as an internal standard The limit of
       detection CLOD) was established at 28 nmol L(-1) and the lower limit of
       quantification (LLOQ) at 85 nmol L(-1) The developed method was applied
       to human plasma samples spiked with repaglinide at therapeutical
       concentrations It was confirmed that the method is suitable for
       pharmacokinetic studies or therapeutic monitoring (C) 2010 Elsevier B V
       All rights reserved,
    DOI = 10.1016/j.jchromb.2010.09.029,
    ISSN = 1570-0232,
    Unique-ID = ISI:000285118100006,
    
  • [DOI] K. Vitkova, J. Petr, V. Maier, J. Znaleziona, and J. Sevcik, “Study of electromigration effects on a pH boundary during the on-line
    electrokinetic preconcentration by capillary electrophoresis,” ELECTROPHORESIS, vol. 31, iss. 16, pp. 2771-2777, 2010.
    [Bibtex]
    @article ISI:000281732900010,
    Author = Vitkova, Katerina and Petr, Jan and Maier, Vitezslav and Znaleziona,
       Joanna and Sevcik, Juraj,
    Title = Study of electromigration effects on a pH boundary during the on-line
       electrokinetic preconcentration by capillary electrophoresis,
    Journal = ELECTROPHORESIS,
    Year = 2010,
    Volume = 31,
    Number = 16,
    Pages = 2771-2777,
    Month = AUG,
    Abstract = A contribution to the description of electrokinetic effects on the pH
       boundary formed by sodium borate pH 9.5 and sodium phosphate pH 2.5
       electrolytes for on-line preconcentration of weak acids is presented in
       this article. Simulations of electrokinetic injections together with
       experimental studies using contactless conductivity detection verified
       that the preconcentration is induced mainly by dissociation changes of
       analytes on the pH boundary and transient ITP state. Moreover, a study
       of the addition of organic solvent to the injection electrolyte was
       performed with impressive results. Subnanomolar LODs of hydroxybenzoic
       acids were achieved with 80\% of methanol in the injection electrolyte
       which represents more than 70 000-fold preconcentration in comparison
       with classical CZE method.,
    DOI = 10.1002/elps.200900663,
    ISSN = 0173-0835,
    Unique-ID = ISI:000281732900010,
    
  • [DOI] R. Knob, J. Marak, A. Stanova, V. Maier, D. Kaniansky, and J. Sevcik, “Determination of brominated phenols in water samples by on-line coupled
    isotachophoresis with capillary zone electrophoresis,” JOURNAL OF CHROMATOGRAPHY A, vol. 1217, iss. 20, pp. 3446-3451, 2010.
    [Bibtex]
    @article ISI:000277893500014,
    Author = Knob, Radim and Marak, Jozef and Stanova, Andrea and Maier, Vitezslav
       and Kaniansky, Dusan and Sevcik, Juraj,
    Title = Determination of brominated phenols in water samples by on-line coupled
       isotachophoresis with capillary zone electrophoresis,
    Journal = JOURNAL OF CHROMATOGRAPHY A,
    Year = 2010,
    Volume = 1217,
    Number = 20,
    Pages = 3446-3451,
    Month = MAY 14,
    Abstract = A method for determination of nine brominated phenols as environmental
       risk compounds was developed by on-line coupled capillary
       isotachophoresis and capillary zone electrophoresis (ITP-CZE). For ITP
       step, 1 x 10(-2) mol L(-1) hydrochloric acid with 3 x 10(-2) mol L(-1)
       ammediol pH 9.1 was used as the leading electrolyte, and 3 x 10(-2) mol
       L(-1) beta-alanine with 2 x 10(-2) mol L(-1) sodium hydroxide pH 10.05
       was used as the terminating electrolyte. As the background electrolyte
       for CZE separation, 2.5 x 10(-2) mol L(-1) beta-alanine with 2.5 x
       10(-2) mol L(-1) lysine pH 9.6 was used. All electrolytes contained
       0.05\% or 0.1\% (m/v) hydroxyethylcellulose to suppress the
       electroosmotic flow. UV detection at wavelength 220nm was used.
       Detection limits in order of tens of nmol L(-1) were achieved. Good
       repeatability of migration times (less than 0.33\% RSD) and good
       repeatability of peak areas (less than 7.19\% RSD) at concentration
       level 5 x 10(-8) mol L(-1) were observed. Developed ITP-CZE method was
       applied to determination of brominated phenols in spiked tap and river
       water samples. (C) 2010 Elsevier B.V. All rights reserved.,
    DOI = 10.1016/j.chroma.2010.03.006,
    ISSN = 0021-9673,
    Unique-ID = ISI:000277893500014,
    
  • [DOI] K. Vitkova, M. Pechova, J. Petr, J. Znaleziona, V. Ranc, V. Maier, and J. Sevcik, “A fast determination of yohimbine in pharmaceuticals by micellar
    electrokinetic chromatography,” CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, vol. 8, iss. 2, pp. 273-277, 2010.
    [Bibtex]
    @article ISI:000276548300005,
    Author = Vitkova, Katerina and Pechova, Marketa and Petr, Jan and Znaleziona,
       Joanna and Ranc, Vaclav and Maier, Vitezslav and Sevcik, Juraj,
    Title = A fast determination of yohimbine in pharmaceuticals by micellar
       electrokinetic chromatography,
    Journal = CENTRAL EUROPEAN JOURNAL OF CHEMISTRY,
    Year = 2010,
    Volume = 8,
    Number = 2,
    Pages = 273-277,
    Month = APR,
    Abstract = A method for the fast determination of yohimbine, a potent
       adrenoreceptor antagonist used for the treatment of sexual dysfunctions,
       is proposed in this article. MEKC under basic and acidic conditions
       (sodium borate, pH 9.5 and sodium phosphate, pH 2.5) with SDS was
       developed. The effect of the experimental parameters, e. g. pH, SDS
       concentration and injection time, on yohimbine migration was also
       studied. Both methods were validated in terms of linearity, limits of
       detection and quantification, accuracy, and precision using caffeine as
       an internal standard. The application for the determination of yohimbine
       in hand-made medicaments is also investigated in this study.,
    DOI = 10.2478/s11532-009-0149-1,
    ISSN = 1895-1066,
    Unique-ID = ISI:000276548300005,
    
  • [DOI] A. Stanova, J. Marak, V. Maier, V. Ranc, J. Znaleziona, J. Sevcik, and D. Kaniansky, “Analysis of buserelin in urine by online combination of capillary zone
    electrophoresis with electrospray mass spectrometry,” ELECTROPHORESIS, vol. 31, iss. 7, SI, pp. 1234-1240, 2010.
    [Bibtex]
    @article ISI:000276811000015,
    Author = Stanova, Andrea and Marak, Jozef and Maier, Vitezslav and Ranc, Vaclav
       and Znaleziona, Joanna and Sevcik, Juraj and Kaniansky, Dusan,
    Title = Analysis of buserelin in urine by online combination of capillary zone
       electrophoresis with electrospray mass spectrometry,
    Journal = ELECTROPHORESIS,
    Year = 2010,
    Volume = 31,
    Number = 7, SI,
    Pages = 1234-1240,
    Month = APR,
    Abstract = A fast and precise analysis of the synthetic peptide buserelin in urine
       using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L
       concentration served as backgroud electrolyte in CZE stage and it is
       compatible with MS detection in positive ionization mode. Two injection
       modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic
       injection (5 kV for 5 s), of which electrokinetic injection provided
       better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water
       and 0 63 mu g/mL in ten times diluted urine samples using pressure
       injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten
       times diluted urine samples using electrokinetic injection Repeatability
       of buserelin migration times was below 6\% (pressure injection mode) and
       1\% (electrokinetic injection mode) Repeatability of buserelin peak area
       in SIM mode (m/z = 620.5 +/- 0.5) was less than 12\% (pressure injection
       mode) and 5 8\% (electrokinetic injection mode). In this work, no
       interferences were observed during the analyses of spiked urine samples.,
    DOI = 10.1002/elps.200900422,
    ISSN = 0173-0835,
    Unique-ID = ISI:000276811000015,
    
  • [DOI] I. Palikova, J. Vostalova, A. Zdarilova, A. Svobodova, P. Kosina, R. Vecera, D. Stejskal, J. Proskova, J. Hrbac, P. Bednar, V. Maier, D. Cernochova, V. Simanek, and J. Ulrichova, “Long-Term Effects of Three Commercial Cranberry Products on the
    Antioxidative Status in Rats: A Pilot Study,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 58, iss. 3, pp. 1672-1678, 2010.
    [Bibtex]
    @article ISI:000274269000041,
    Author = Palikova, Irena and Vostalova, Jitka and Zdarilova, Adela and Svobodova,
       Alena and Kosina, Pavel and Vecera, Rostislav and Stejskal, David and
       Proskova, Jitka and Hrbac, Jan and Bednar, Petr and Maier, Vitezslav and
       Cernochova, Drahomira and Simanek, Vilim and Ulrichova, Jitka,
    Title = Long-Term Effects of Three Commercial Cranberry Products on the
       Antioxidative Status in Rats: A Pilot Study,
    Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
    Year = 2010,
    Volume = 58,
    Number = 3,
    Pages = 1672-1678,
    Month = FEB 10,
    Abstract = Cranberry (Vaccinium macrocarpon Ait. Ericaceae) fruits and juice are
       widely used for their antiadherence and antioxidative properties. Little
       is known however about their effects on clinical chemistry markers after
       long-term consumption. This study was conducted to evaluate the effect
       of three commercial cranberry products, NUTRICRAN90S, HI-PAC 4.0, and
       PACRAN on the antioxidative status of rodents, divided into three
       experimental groups. The products were given as dietary admixtures (1500
       mg of product/kg of stock feed) for 14 weeks to male Wistar rats (Groups
       2-4) and a control Group 1 which received only stock feed. There were no
       significant cranberry treatment-related effects on oxidative stress
       parameters, catalase, glutathione peroxidase, glutathione reductase,
       glutathione transferase, superoxide dismutase, total antioxidant
       capacity, thiobarbituric acid reactive substances, advanced oxidation
       protein products, total SH-groups, or any other measured clinical
       chemistry markers. Hematological parameters, body weight, and food
       consumption were also unaffected by intake of cranberries. Only liver
       glutathione reductase activity and glutathione levels were significantly
       lower in Group 4 than in Group 1. Plasma alkaline phosphatase alone was
       significantly decreased in Group 2. No gross pathology, effects on organ
       weights, or histopathology were observed. No genotoxicity was found, and
       total cytochrome P450 level in liver was unaffected in all groups. The
       levels of hippuric acid and several phenolic acids were significantly
       increased in plasma and urine in Groups 2-4. The concentration of
       anthocyanins was under the detection threshold. The dietary addition of
       cranberry powders for 14 weeks was well tolerated, but it did not
       improve the antioxidative status in rats.,
    DOI = 10.1021/jf903710y,
    ISSN = 0021-8561,
    Unique-ID = ISI:000274269000041,
    
  • J. Skopalova, D. Jirovsky, Z. Bartosova, and V. Maier, “Electrochemical Behavior of Antidiabetics Repaglinide and Rosiglitazone
    and their Determination by Liquid Chromatography with Electrochemical
    Detection,” in MODERN ELECTROCHEMICAL METHODS XXX, 2010, pp. 149-153.
    [Bibtex]
    @inproceedings ISI:000283050900034,
    Author = Skopalova, Jana and Jirovsky, David and Bartosova, Zdenka and Maier,
       Vitezslav,
    Editor = Navratil, T and Jiri, B,
    Title = Electrochemical Behavior of Antidiabetics Repaglinide and Rosiglitazone
       and their Determination by Liquid Chromatography with Electrochemical
       Detection,
    Booktitle = MODERN ELECTROCHEMICAL METHODS XXX,
    Year = 2010,
    Pages = 149-153,
    Note = 30th Modern Electrochemical Methods, Jetrichovice, CZECH REPUBLIC, MAY
       24-28, 2010,
    Abstract = A voltammetric behavior of two antidiabetic drugs repaglinide and
       rosiglitazone was investigated on glassy carbon electrode using cyclic
       and differential pulse voltammetry. Oxidation of the both drugs occurred
       in the entire tested pH range of 2-9 giving well defined anodic current
       responses. Suitable conditions for coulometric detection of the drugs at
       porous-carbon electrode were found on the basis of voltammetric
       experiments. A simple and highly sensitive reverse-phase liquid
       chromatography method with coulometric detection was developed and
       applied to the determination of nanomolar concentractions of the drugs
       in spiked human plasma.,
    ISBN = 978-80-254-6710-7,
    Unique-ID = ISI:000283050900034,
    

2009

  • [DOI] J. Petr, O. Ryparova, J. Znaleziona, V. Maier, and J. Sevcik, “On-line combination of CE and microscopy: An insight into the migration
    of microorganisms,” ELECTROPHORESIS, vol. 30, iss. 22, pp. 3863-3869, 2009.
    [Bibtex]
    @article ISI:000272474900009,
    Author = Petr, Jan and Ryparova, Olga and Znaleziona, Joanna and Maier, Vitezslav
       and Sevcik, Juraj,
    Title = On-line combination of CE and microscopy: An insight into the migration
       of microorganisms,
    Journal = ELECTROPHORESIS,
    Year = 2009,
    Volume = 30,
    Number = 22,
    Pages = 3863-3869,
    Month = NOV,
    Abstract = identification of microbial contamination by CE has interested many
       researchers mainly because of the high speed of CE analysis. However,
       the CE separation of such big structures brings a lot of questions
       mainly about the behavior of microorganisms and about the mechanism of
       separation. In this work, we constructed a simple apparatus where a
       microscope was used as one detector and a UV detector was used as the
       second one and we made the comparison of three typical setups for CE of
       microorganisms. Saccharomyces cerevisiae was chosen as a model
       microorganism and was analyzed in bare fused-silica capillaries,
       covalently modified capillaries and dynamically modified capillaries by
       poly(ethylene oxide) or CTAB. Results showed that the use of CE
       instrument directly connected with a microscope could be advantageous
       for the study of separation mechanisms and/or migration behavior.,
    DOI = 10.1002/elps.200900230,
    ISSN = 0173-0835,
    Unique-ID = ISI:000272474900009,
    
  • [DOI] V. Maier, J. Znaleziona, D. Jirovsky, J. Skopalova, J. Petr, and J. Sevcik, “Determination of antihyperglycemic drugs in nanomolar concentration
    levels by micellar electrokinetic chromatography with non-ionic
    surfactant,” JOURNAL OF CHROMATOGRAPHY A, vol. 1216, iss. 20, pp. 4492-4498, 2009.
    [Bibtex]
    @article ISI:000265956100025,
    Author = Maier, Vitezslav and Znaleziona, Joanna and Jirovsky, David and
       Skopalova, Jana and Petr, Jan and Sevcik, Juraj,
    Title = Determination of antihyperglycemic drugs in nanomolar concentration
       levels by micellar electrokinetic chromatography with non-ionic
       surfactant,
    Journal = JOURNAL OF CHROMATOGRAPHY A,
    Year = 2009,
    Volume = 1216,
    Number = 20,
    Pages = 4492-4498,
    Month = MAY 15,
    Abstract = A method for the separation of six selected antihyperglycemic
       (antidiabetic) drugs (tolbutamide, gliclazide, glimepiride,
       glibenclamide, repaglinide, and glipizide) was developed with use of
       micellar electrokinetic chromatography. Two non-ionic poly(ethylene
       glycol)-based surfactants Genapol X-080 and Triton X-114 (reduced) were
       studied as neutral pseudostationary phases. High alkaline pH 10.0 was
       used to obtain negative charges of separated antidiabetic drugs and
       non-ionic surfactants were employed for selectivity alteration. Both
       non-ionic surfactants provided good selectivity at concentration 0.2\%
       (v/v) in sodium borate buffer and the separation of six drugs was
       obtained within 5 min. An on-line preconcentration method based on
       reversed electrode polarity switching was employed for the determination
       of anti hyperglycemic drugs in blood serum after acetonitrile protein
       precipitation. The limits of detection ranged from 20.8 nmol L-(1) for
       tolbutamide to 6.5 nmol L(-1) for glibenclamide, respectively. (C) 2009
       Elsevier B.V. All rights reserved.,
    DOI = 10.1016/j.chroma.2009.03.052,
    ISSN = 0021-9673,
    Unique-ID = ISI:000265956100025,
    
  • [DOI] J. Petr, O. Ryparova, V. Ranc, P. Hinnerova, J. Znaleziona, M. Kowalska, R. Knob, V. Maier, I. Frebort, K. Lemr, and J. Sevcik, “Assessment of CE for the identification of microorganisms,” ELECTROPHORESIS, vol. 30, iss. 3, pp. 444-449, 2009.
    [Bibtex]
    @article ISI:000264357500002,
    Author = Petr, Jan and Ryparova, Olga and Ranc, Vaclav and Hinnerova, Petra and
       Znaleziona, Joanna and Kowalska, Marta and Knob, Radim and Maier,
       Vitezslav and Frebort, Ivo and Lemr, Karel and Sevcik, Juraj,
    Title = Assessment of CE for the identification of microorganisms,
    Journal = ELECTROPHORESIS,
    Year = 2009,
    Volume = 30,
    Number = 3,
    Pages = 444-449,
    Month = FEB,
    Abstract = The assessment of capillary electrophoresis for separation and
       identification of microorganisms is described in this article. The work
       brings a comparison of the application of uncoated capillaries modified
       with poly(ethylene oxide) and coated capillaries for the separation of
       model microorganisms Saccharomyces cerevisiae and Escherichia coli with
       Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best
       separation was achieved in the coated capillary using 1 mM
       Tris-citrate-fructose buffer, pH 6.9. A simple identification based on
       the migration time and UV spectrum was found unsatisfactory and thus a
       method based on a post-separation cultivation and sequential analysis
       was developed. Off-line combination with mass spectrometric analysis
       with the use of desorption electrospray was shown to be an interesting
       alternative in a study of microorganisms. Mass spectra allowed
       distinguishing among analyzed cells.,
    DOI = 10.1002/elps.200800232,
    ISSN = 0173-0835,
    Unique-ID = ISI:000264357500002,
    
  • J. Petr, V. Maier, J. Znaleziona, K. Vitkova, V. Ranc, and J. Sevcik, “On-line Preconcentration on pH Boundary – the Beauty of Capillary
    Electrophoresis,” CHEMICKE LISTY, vol. 103, iss. 8, pp. 661-666, 2009.
    [Bibtex]
    @article ISI:000269243500006,
    Author = Petr, Jan and Maier, Vitezslav and Znaleziona, Joanna and Vitkova,
       Katerina and Ranc, Vaclav and Sevcik, Juraj,
    Title = On-line Preconcentration on pH Boundary - the Beauty of Capillary
       Electrophoresis,
    Journal = CHEMICKE LISTY,
    Year = 2009,
    Volume = 103,
    Number = 8,
    Pages = 661-666,
    Abstract = The beauty of capillary electrophoresis is shown on preconcentration of
       some phenolics. The method is based on the electrokinetic injection of
       phenolics into a sodium borate electrolyte (pH 9.5) at the pH boundary
       formed by sodium phosphate (pH 2.5) followed by mobilization and
       separation by replacing the injection vial with the vial containing
       micellar environment. This technique allows determination of nanomolar
       concentrations of phenolics.,
    ISSN = 0009-2770,
    Unique-ID = ISI:000269243500006,
    

2008

  • [DOI] J. Petr, K. Vitkova, V. Ranc, J. Znaleziona, V. Maier, R. Knob, and J. Sevcik, “Determination of some phenolic acids in Majorana hortensis by capillary
    electrophoresis with online electrokinetic preconcentration,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 56, iss. 11, pp. 3940-3944, 2008.
    [Bibtex]
    @article ISI:000256445300008,
    Author = Petr, Jan and Vitkova, Katerina and Ranc, Vaclav and Znaleziona, Joanna
       and Maier, Vitezslav and Knob, Radim and Sevcik, Juraj,
    Title = Determination of some phenolic acids in Majorana hortensis by capillary
       electrophoresis with online electrokinetic preconcentration,
    Journal = JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,
    Year = 2008,
    Volume = 56,
    Number = 11,
    Pages = 3940-3944,
    Month = JUN 11,
    Abstract = An online accumulation/mobilization preconcentration technique based on
       a dynamic pH junction technique and electrokinetic injection was
       employed for analysis of phenolic acids (sinapic; ferulic, coumarinic,
       caffeic, syringic, vanillic, and 4-hydroxybenzoic acid) in extracts from
       Majorana hortensis leaves. Samples were extracted by pressurized solvent
       extraction with acetone at 150 degrees C and 15 MPa. The capillary
       electrophoretic method employed 50 mmol.L-1 sodium borate, pH 9.5, as
       the sample electrolyte, 50 mmol.L-1 sodium phosphate, pH 2.5, as the
       background electrolyte, and 50 mmol.L-1 sodium phosphate, pH 2.5, with
       60 mmol.L-1 sodium dodecyl sulfate as the mobilization electrolyte. The
       method allowed 720-fold to 5560-fold preconcentration of the phenolic
       acids during 30 min of electrokinetic accumulation with detection limits
       from 0.38 to 4.22 ng.mL(-1).,
    DOI = 10.1021/jf8000179,
    ISSN = 0021-8561,
    Unique-ID = ISI:000256445300008,
    
  • [DOI] J. Znaleziona, J. Petr, R. Knob, V. Maier, and J. Sevcik, “Dynamic coating agents in CE,” CHROMATOGRAPHIA, vol. 67, iss. S, pp. S5-S12, 2008.
    [Bibtex]
    @article ISI:000256533700003,
    Author = Znaleziona, Joanna and Petr, Jan and Knob, Radim and Maier, Vitezslav
       and Sevcik, Juraj,
    Title = Dynamic coating agents in CE,
    Journal = CHROMATOGRAPHIA,
    Year = 2008,
    Volume = 67,
    Number = S,
    Pages = S5-S12,
    Note = 10th Symposium on Advances in Chromatography and Electrophoresis and
       Chiranal, Palacky Univ, Olomouc, CZECH REPUBLIC, JUN 24-27, 2007,
    Organization = Czech Chem Soc, Working Grp Chromatog \& Electrophoresis,
    Abstract = In capillary electrophoresis (CE) dynamic coating agents are commonly
       used for manipulating or reversing electroosmotic flow (EOF) and
       suppressing adsorption of analytes by the silica surface. In this paper
       we review general aspects of dynamic coating in CE and discuss features
       and facts concerning formation of the EOF and determination of its
       magnitude.,
    DOI = 10.1365/s10337-007-0509-y,
    ISSN = 0009-5893,
    Unique-ID = ISI:000256533700003,
    
  • O. Ryparova, J. Petr, M. Kowalska, J. Znaleziona, R. Knob, V. Maier, I. Frebort, and J. Sevcik, “Analysis of Microorganisms by Capillary Electrophoresis,” CHEMICKE LISTY, vol. 102, iss. 12, pp. 1121-1126, 2008.
    [Bibtex]
    @article ISI:000262061500007,
    Author = Ryparova, Olga and Petr, Jan and Kowalska, Marta and Znaleziona, Joanna
       and Knob, Radim and Maier, Vitezslav and Frebort, Ivo and Sevcik, Juraj,
    Title = Analysis of Microorganisms by Capillary Electrophoresis,
    Journal = CHEMICKE LISTY,
    Year = 2008,
    Volume = 102,
    Number = 12,
    Pages = 1121-1126,
    Abstract = Utilization of capillary electrophoresis for screening identification of
       Escherichia coli in water (faecal contamination) and pharmaceuticals is
       described. The method uses an electrolyte 4.5 mM in Tris, 4.5 mM in
       H(3)BO(3) and 0.1 mM in EDTA with 0.0125 \% poly(ethylene oxide). A
       brief survey of the published methods for analysis of microorganisms by
       capillary electrophoresis is given.,
    ISSN = 0009-2770,
    Unique-ID = ISI:000262061500007,
    

2007

  • [DOI] J. Horakova, J. Petr, V. Maier, J. Znaleziona, A. Stanova, J. Marak, D. Kaniansky, and J. Sevcik, “Combination of large volume sample stacking and dynamic pH junction for
    on-line preconcentration of weak electrolytes by capillary
    electrophoresis in comparison with isotachophoretic techniques,” JOURNAL OF CHROMATOGRAPHY A, vol. 1155, iss. 2, pp. 193-198, 2007.
    [Bibtex]
    @article ISI:000247855200010,
    Author = Horakova, Jana and Petr, Jan and Maier, Vitezslav and Znaleziona, Joanna
       and Stanova, Andrea and Marak, Jozef and Kaniansky, Dusan and Sevcik,
       Juraj,
    Title = Combination of large volume sample stacking and dynamic pH junction for
       on-line preconcentration of weak electrolytes by capillary
       electrophoresis in comparison with isotachophoretic techniques,
    Journal = JOURNAL OF CHROMATOGRAPHY A,
    Year = 2007,
    Volume = 1155,
    Number = 2,
    Pages = 193-198,
    Month = JUL 6,
    Note = 15th International Symposium on Capillary Electroseparation Techniques,
       Paris, FRANCE, AUG 28-30, 2006,
    Abstract = An on-line preconcentration capillary electrophoresis (CE) technique,
       which combines a large volume sample stacking with a dynamic pH junction
       technique, is introduced in this paper. This dynamic pH junction with
       co-electroosmotic migration is formed between sodium borate pH 9.5 and
       sodium phosphate pH 2.5 with 150 mM sodium dodecylsulfate (SDS). A full
       capillary based injection allows determination of weak acidic compounds
       at ppb concentration levels (achieved LOD for benzoic acid was 11 nmol
       L-1). The proposed preconcentration method was compared with ITP/ITP
       (LOD 120 nmol L-1), ITP/CZE (LOD 740 nmol L-1) and a simple CZE method
       (LOD 23,330 nmol L-1). The analytical potential of this method was
       assessed with juice test samples. (C) 2007 Elsevier B.V. All rights
       reserved.,
    DOI = 10.1016/j.chroma.2007.01.075,
    ISSN = 0021-9673,
    Unique-ID = ISI:000247855200010,
    
  • [DOI] V. Maier, J. Petr, R. Knob, J. Horakova, and J. Sevcik, “Electrokinetic partial filling technique as a powerful tool for
    enantiomeric separation of DL-lactic acid by CE with contactless
    conductivity detection,” ELECTROPHORESIS, vol. 28, iss. 11, pp. 1815-1822, 2007.
    [Bibtex]
    @article ISI:000247279800020,
    Author = Maier, Vitezslav and Petr, Jan and Knob, Radim and Horakova, Jana and
       Sevcik, Juraj,
    Title = Electrokinetic partial filling technique as a powerful tool for
       enantiomeric separation of DL-lactic acid by CE with contactless
       conductivity detection,
    Journal = ELECTROPHORESIS,
    Year = 2007,
    Volume = 28,
    Number = 11,
    Pages = 1815-1822,
    Month = JUN,
    Abstract = A modified partial filling method for chiral separation Of DL-lactic
       acid as the model chiral compound with vancomycin chloride as the chiral
       selector was developed by CE with contactless conductivity detection.
       Electrokinetic partial filling technique (EK-PFT) was used as an
       altemative method to the conventional hydrodynamic partial filling
       method. EK-PFT, in contrast to the hydrodynamic partial filling
       technique, allowed the removal of the chloride counterions from the
       chiral selector which otherwise led to poor sensitivity in conductivity
       detection. The baseline separation Of DL-lactic acid as the model
       analyte was achieved in 5 min in a polyacrylamide-coated capillary. The
       best resolution was achieved by electrokinetic partial filling of
       vancomycin cations from the injection solution containing 5 nunol/L
       oxalate L-histidiniumat pH 4.5 with 10 mmol/L vancomycin chloride.
       Computer simulation was used to explain the observed phenomena in the
       boundary between the inject vial and the capillary during the EK-PFT of
       vancomycin cations.,
    DOI = 10.1002/elps.200600578,
    ISSN = 0173-0835,
    Unique-ID = ISI:000247279800020,
    
  • [DOI] J. Horakova, J. Petr, V. Maier, E. Tesarova, L. Veis, D. W. Armstrong, B. Gas, and J. Sevcik, “On-line preconcentration of weak electrolytes by electrokinetic
    accumulation in CE: Experiment and simulation,” ELECTROPHORESIS, vol. 28, iss. 10, pp. 1540-1547, 2007.
    [Bibtex]
    @article ISI:000246923300011,
    Author = Horakova, Jana and Petr, Jan and Maier, Vitezslav and Tesarova, Eva and
       Veis, Libor and Armstrong, Daniel W. and Gas, Bohuslav and Sevcik, Juraj,
    Title = On-line preconcentration of weak electrolytes by electrokinetic
       accumulation in CE: Experiment and simulation,
    Journal = ELECTROPHORESIS,
    Year = 2007,
    Volume = 28,
    Number = 10,
    Pages = 1540-1547,
    Month = MAY,
    Abstract = A new on-line preconcentration technique was developed that makes
       possible to determine nanomolar concentrations of weak acidic analytes
       in CE. The method consists of long-running electrokinetic sample
       injection and stacking (electrokinetic immobilization) of the analytes
       at a boundary of two electrolytes with different pH values (pH 9.5 and
       2.5) and consequent mobilization of the stacked uncharged analytes in a
       micellar system (containing SDS micelles). Several factors including
       buffer concentration, pH, applied voltage, time of preconcentration, and
       SDS concentration were tested to optimize the analysis method. An about
       4600-fold increase of the sample concentration (in comparison with the
       standard CZE) can be achieved during the preconcentration step. Two
       preservatives applied in food industry - benzoic acid and sorbic acid
       were used as model samples. The applicability of the proposed method in
       food analysis was demonstrated by determination of nanomolar
       concentrations of benzoic acid in sunflower oil. An extended version of
       the computer program Simul was used for modeling both the
       preconcentration and mobilization processes taking place in the
       capillary.,
    DOI = 10.1002/elps.200600681,
    ISSN = 0173-0835,
    Unique-ID = ISI:000246923300011,
    
  • [DOI] D. Friedecky, J. Tomkova, V. Maier, A. Janost’akova, M. Prochazka, and T. Adam, “Capillary electrophoretic method for nucleotide analysis in cells:
    Application on inherited metabolic disorders,” ELECTROPHORESIS, vol. 28, iss. 3, pp. 373-380, 2007.
    [Bibtex]
    @article ISI:000244425600014,
    Author = Friedecky, David and Tomkova, Jana and Maier, Vitezslav and
       Janost'akova, Anna and Prochazka, Martin and Adam, Tomas,
    Title = Capillary electrophoretic method for nucleotide analysis in cells:
       Application on inherited metabolic disorders,
    Journal = ELECTROPHORESIS,
    Year = 2007,
    Volume = 28,
    Number = 3,
    Pages = 373-380,
    Month = FEB,
    Abstract = Purine and pyrimidine nucleotides influence many metabolic pathways and
       their analogs have been widely used in medicine. A capillary
       electrophoretic method was developed for measuring intracellular
       nucleotides. The final BGE consisted of 40 mM citric acid with addition
       of 0.8 mM CTAB titrated by gamma-aminobutyric acid to pH 4.4. The
       electrophoretic separations were carried out in an uncoated silica
       capillary (id/od - 75/375 mu m; effective/total length - 90/97 cm). The
       method allows a complete separation of 21 nucleotides and
       deoxynucleotides within 15 min with separation efficiencies up to 400
       000 theoretical plates per meter. Due to the use of an acidic separation
       medium, the method offers a high selectivity toward the studied analytes
       versus possible interferences from matrices. Sample preparation was
       optimized in order to shorten work-time and prevent analyte degradation.
       The method was applied for analyzing nucleotides in human erythrocytes
       and Chinese hamster ovary cells. Diagnostic potential for inherited
       metabolic disorders of nucleotide metabolism is presented.,
    DOI = 10.1002/elps.200600262,
    ISSN = 0173-0835,
    Unique-ID = ISI:000244425600014,
    

2006

  • [DOI] J. Petr, V. Maier, J. Horakova, J. Sevcik, and Z. Stransky, “Capillary isotachophoresis from the student point of view – images and
    the reality,” JOURNAL OF SEPARATION SCIENCE, vol. 29, iss. 18, pp. 2705-2715, 2006.
    [Bibtex]
    @article ISI:000243338800002,
    Author = Petr, Jan and Maier, Vitezslav and Horakova, Jana and Sevcik, Juraj and
       Stransky, Zdenek,
    Title = Capillary isotachophoresis from the student point of view - images and
       the reality,
    Journal = JOURNAL OF SEPARATION SCIENCE,
    Year = 2006,
    Volume = 29,
    Number = 18,
    Pages = 2705-2715,
    Month = DEC,
    Abstract = A review of some fundamental aspects of ITP from the student point of
       view, imaginations of some basic facts and laws, use of ITP, and the
       recent trends are presented. The results of theoretical computations of
       ITP separation processes are added for comparison of imaginations with
       the exact mathematical description.,
    DOI = 10.1002/jssc.200600249,
    ISSN = 1615-9306,
    Unique-ID = ISI:000243338800002,
    
  • [DOI] J. Petr, V. Maier, J. Horakova, and J. Sevcik, “Simultaneous contactless conductivity detection and UV detection for the
    study of separation of tamsulosin enantiomers in discontinuous
    electrolyte systems by CE,” ELECTROPHORESIS, vol. 27, iss. 23, pp. 4735-4745, 2006.
    [Bibtex]
    @article ISI:000242903000017,
    Author = Petr, Jan and Maier, Vitezslav and Horakova, Jana and Sevcik, Juraj,
    Title = Simultaneous contactless conductivity detection and UV detection for the
       study of separation of tamsulosin enantiomers in discontinuous
       electrolyte systems by CE,
    Journal = ELECTROPHORESIS,
    Year = 2006,
    Volume = 27,
    Number = 23,
    Pages = 4735-4745,
    Month = DEC,
    Abstract = This work shows the potential of using discontinuous electrolyte systems
       for the separation of tamsulosin enantiomers by CE. Sulfated
       P-cyclodextrin was used as a chiral selector. In acidic electrolytes,
       sulfated P-cyclodextrin migrates as an anion and the analyte
       (tamsulosin) migrates as a cation. Due to this, four experimental
       arrangements were proposed. These arrangements differ in composition of
       electrolytes in the inlet compartment, in the capillary and in the
       outlet compartment. The separation of tamsulosin enantiomers in acetate
       buffers with sodium and Tris counterions was studied. Simultaneous
       contactless conductivity detection and UV detection were used for the
       study of the separation mechanism in these systems. Mobilities of
       sulfated beta-cycloclextrin were used for the calculation of the time
       when the analyte migrates through the BGE zone with the selector. The
       simulation program Simul 4.0 was used for the calculations of the
       concentration profiles of the electrolyte components dependent on the
       time of the separation. The mechanism of enantioseparation in these
       arrangements was suggested.,
    DOI = 10.1002/elps.200600063,
    ISSN = 0173-0835,
    Unique-ID = ISI:000242903000017,
    
  • [DOI] V. Maier, J. Horakova, J. Petr, D. Drahonovsky, and J. Sevcik, “Using of S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium
    tetrafluoroborate as additive to the background electrolyte in capillary
    electrophoresis,” JOURNAL OF CHROMATOGRAPHY A, vol. 1103, iss. 2, pp. 337-343, 2006.
    [Bibtex]
    @article ISI:000234879600019,
    Author = Maier, V and Horakova, J and Petr, J and Drahonovsky, D and Sevcik, J,
    Title = Using of S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium
       tetrafluoroborate as additive to the background electrolyte in capillary
       electrophoresis,
    Journal = JOURNAL OF CHROMATOGRAPHY A,
    Year = 2006,
    Volume = 1103,
    Number = 2,
    Pages = 337-343,
    Month = JAN 27,
    Abstract = We synthesised and used new type of quaternary ammonium salt
       [S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium tetrafluoroborate] as
       effective additive to acidic background electrolytes. We used this
       quaternary ammonium salt as effective agent for capillary zone
       electrophoresis separation of model mixture of five tricyclic
       antidepressants (amitriptyline, nortriptyline, imipramine, desipramine
       and clomipramine) as model analytes. We observed that addition of
       S-(-)-2-hydroxymethyl-1,1-dimethylpyrrolidinium tetrafluoroborate
       ([HMDP](+) [BF4](-)) to acidic background electrolytes leads to
       suppression of magnitude of electroosmotic flow (EOF) and gradually
       change the direction of the EOF. Baseline separation of five TAs was
       achieved by using of 91.1 mmol L-1 (20 g L-1) of [HMDP](+) [BF4](-)
       in 25 mmol L-1 sodium phosphate pH 2.5, where electroosmotic mobility
       was -11.3 x 10(-9) m(2) V-1 s(-1). We achieved baseline separation of
       five TAs with using of [HMDP](+) [BF4](-) as water solution too. We
       observed that [HMDP](+) [BF4](-) Can be used as buffer additive,
       which offers relatively smaller anodic electroosmotic flow instead of
       cationic surfactants that are mostly used for genarating of anodic
       electroosmotic flow in capillary electrophoresis. (c) 2005 Elsevier B.V.
       All fights reserved.,
    DOI = 10.1016/j.chroma.2005.11.011,
    ISSN = 0021-9673,
    Unique-ID = ISI:000234879600019,
    
  • J. Horakova, V. Maier, and J. Sevcik, “On-line preconcentration techniques in capillary electrophoresis,” CHEMICKE LISTY, vol. 100, iss. 3, pp. 163-168, 2006.
    [Bibtex]
    @article ISI:000236139700002,
    Author = Horakova, J and Maier, V and Sevcik, J,
    Title = On-line preconcentration techniques in capillary electrophoresis,
    Journal = CHEMICKE LISTY,
    Year = 2006,
    Volume = 100,
    Number = 3,
    Pages = 163-168,
    Abstract = Preconcentration is a very important issue for achievement of low
       detection limits. Various on-line preconcentration modes, various
       electrolyte compositions, pH values and mechanisms responsible for the
       focusing effect are described in literature. The main modes of stacking,
       sweeping and transient isotachophoresis are discussed. Some of the
       described methods are combinations of major techniques of
       preconcentration and less known focusing techniques. References for
       optimization of focusing conditions and routine use of on-line
       preconcentration are given.,
    ISSN = 0009-2770,
    Unique-ID = ISI:000236139700002,
    

2005

  • [DOI] V. Maier, J. Horakova, J. Petr, E. Tesarova, P. Coufal, and J. Sevcik, “Chiral separation of tamsulosin by capillary electrophoresis,” JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 39, iss. 3-4, pp. 691-696, 2005.
    [Bibtex]
    @article ISI:000232209700047,
    Author = Maier, V and Horakova, J and Petr, J and Tesarova, E and Coufal, P and
       Sevcik, J,
    Title = Chiral separation of tamsulosin by capillary electrophoresis,
    Journal = JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,
    Year = 2005,
    Volume = 39,
    Number = 3-4,
    Pages = 691-696,
    Month = SEP 15,
    Abstract = Enantiomers of (+/-) 5-[2 (R,S)-\[2-(o-ethoxyphenoxy) ethyl]
       amino\ propyl]-2-methoxy-benzenesulfonamide (tamsulosin, drug
       frequently used in the treatment of prostate diseases) were separated by
       capillary electrophoresis (CE). An acidic background electrolyte (BGE)
       with sulfated-beta-cyclodextrin (S-P-CD) was used to create a chiral
       separation environment. Baseline separation of the isomers was achieved
       during 5 min using cathodic electro-osmotic flow (EOF) (countercurrent
       mode). The quantification limits were 5.3 x 10(-6) Mol l(-1) for
       R-isomer and 5.7 x 10(-6) Mol l(-1) for S-isomer. The R.S.D. values of
       peak area were 0.54\% for R-isomer and 0.75\% for S-isomer. The results
       achieved enable determination of 0.5\% of optical impurity. (c) 2005
       Elsevier B.V. All rights reserved.,
    DOI = 10.1016/j.jpba.2005.04.023,
    ISSN = 0731-7085,
    Unique-ID = ISI:000232209700047,
    
  • J. Petr, V. Maier, J. Horakova, E. Tesarova, and J. Sevcik, “How to achieve successful chiral separation by capillary electrophoresis,” CHEMICKE LISTY, vol. 99, iss. 3, pp. 190-194, 2005.
    [Bibtex]
    @article ISI:000227483400007,
    Author = Petr, J and Maier, V and Horakova, J and Tesarova, E and Sevcik, J,
    Title = How to achieve successful chiral separation by capillary electrophoresis,
    Journal = CHEMICKE LISTY,
    Year = 2005,
    Volume = 99,
    Number = 3,
    Pages = 190-194,
    Abstract = This work is devoted to enhancing the potential of capillary
       electrophoresis as a separation technique. Improvement of chiral
       resolution of organic bases in an acid electrolyte using sulfated
       P-cyclodextrin as an anionic chiral selector is demonstrated on an
       example of chiral separation of tamsulosin and ephedrine. Better
       resolution of enantiomers; of the bases can be achieved by changing the
       concentration of a chiral selector in compartments of the separation
       system (inlet - capillary - outlet). Migration of the chiral selector in
       the direction opposite to that of the cationic analytes was utilized and
       thus the resolution could be improved. The best resolution was obtained
       if the chiral selector was in the working electrolyte in the capillary
       and outlet vial while the inlet vial contained only the background
       electrolyte. In contrast, if the chiral selector is either only in the
       capillary or in the whole electrophoresis system, a lower resolution was
       obtained though both the systems are most frequently employed in
       practice.,
    ISSN = 0009-2770,
    Unique-ID = ISI:000227483400007,