Publication in Inorganic Chemistry

Anticancer Potential of Diruthenium Complexes with Bridging Hydrocarbyl Ligands from Bioactive Alkynols


Authors: Giulio Bresciani, Ján Vančo, Tiziana Funaioli, Stefano Zacchini, Tomáš Malina, Guido Pampaloni, Zdeněk Dvořák, Zdeněk Trávníček,* and Fabio Marchetti*



The work was realized based on the collaboration with the group of prof. Fabio Marchetti from the University of Pisa, Italy. The diruthenacyclopentenone complexes of the general composition [Ru2Cp2(CO)2{μ–η13-CH═C(C(OH)(R))C(═O)}] (Cp = η5-C5H5) were synthesized and thoroughly characterized. The in vitro cytotoxicity of the compounds was evaluated against nine human cancer cell lines (A2780, A2780R, MCF-7, HOS, A549, PANC-1, Caco-2, PC-3, and HeLa), while the selectivity was assessed on normal human lung fibroblast (MRC-5). Overall, the complexes exert stronger cytotoxicity than cisplatin. ICP-mass spectrometry cellular uptake studies in A2780 cells were performed. The cellular effects of the complexes on A2780 cancer cells were studied using flow cytometry, revealing their impact on cell cycle, apoptosis, oxidative stress, autophagy and mitochondrial membrane potential depletion.